Difference between revisions of "Team:SVCE Chennai/Safety"

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{{SVCE_Chennai}}
 
 
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<h2>Safety in iGEM</h2>
 
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<meta name="apple-mobile-web-app-capable" content="yes">
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<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<link rel="stylesheet" type="text/css" href="https://bootswatch.com/flatly/bootstrap.css"/>
  
  
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<h4>Safe Project Design</h4>
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:SVCE_Chennai/CSS?action=raw&ctype=text/css" />
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
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<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.4.0/css/font-awesome.min.css">
  
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<ul>
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<nav class="navBarIGEM navbar navbar-default navbar-fixed-top">
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h4>Safe Lab Work</h4>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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    <div class="container">
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        <h2 class="titleStyle">Safety</h2>
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            <h5>Table of Contents</h5><hr class="no-margin">
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                    <a href="#intro">
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                        <span class="fa fa-angle-double-right text-primary"></span>Introduction
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                    </a>
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                </li>
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                <li>
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                    <a href="#projsafety">
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                        <span class="fa fa-angle-double-right text-primary"></span>Project Safety
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                <li>
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                    <a href="#labsafety">
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                        <span class="fa fa-angle-double-right text-primary"></span>Lab Safety
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        <h3 id="intro" class="title">Introduction</h3>
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        <p>Laboratories are often recognized with hazards of processing infectious agents. Safety measures were
  
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<h4>Safe Shipment</h4>
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carried out by the team by providing guidelines, engineering controls, management policies and work
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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practices. We followed a high standard of safe and responsible biological engineering. It is one of the primary
  
 +
concerns of the team and we ensured that all the works carried are highly safe. In general, iGEM teams are trying
  
 +
            to pursue ambitious projects and reduce risks by using safer substitutes for more dangerous organisms/parts.</p>
 +
        <h3 id="projsafety" class="title">Project Safety</h3>
 +
        <p>Our project which focuses on the production of minicins in which we mainly target gastro intestinal infections and
 +
 +
biofilm formation though are mostly caused by hazardous organisms such as Shigella, Clostridium etc., Our
 +
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team worked on class 2 organisms such as Pseudomonas aeruginosa and Staphylococcus aureus as model
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organism to target the same with reduced risks and have a safe project design .</p>
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<table class="table table-bordered">
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        <thead><tr><th>ORGANISM</th><th>RISK GROUP</th></tr></thead>
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    <tbody>
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        <tr><td>E. coli BL21</td><td>1</td></tr>
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        <tr><td>Pseudomonas aeruginosa</td><td>2</td></tr>
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        <tr><td>Staphylococcus aureus</td><td>2</td></tr>
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    </tbody></table>
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        <p>As our college is provided with well equipped class1 and class 2 cabinets ,which made it easier to handle these
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 +
organisms. Proper bio safety guidelines were also considered for cloning and synthetic biology works .</p>
 +
       
 +
        <h3 id="labsafety" class="title">Lab Safety</h3>
 +
       
 +
        <p style="padding-bottom:100px;">We the team members have taken special online Good Microbiological Laboratory Practices before the project
 +
 +
was initiated. Though we reduced the risk hazards of the project by working on class 1 & class 2, safety is
 +
 +
essential all the time. The lab team concentrates more on the safety of handling organisms and parts used in the
 +
 +
project. All the safety cabinets and labs used for the project are well furnished with separate rooms for the
 +
 +
cabinets . Safety regarding chemicals used in the project is also considered, especially for Etbr, agarose and
 +
 +
other carcinogens which were always handled with gloves. The containment is also well autoclaved and discarded
 +
 +
by proper disposables to prevent risks to the environment . All the safety measures followed by the team were
 +
 +
guided by our instructors and we have also ensured that the final product of the work produces no risk or reduced
 +
 +
            risk to the environment and other living organisms.</p>
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Latest revision as of 03:21, 19 September 2015

Safety

Introduction

Laboratories are often recognized with hazards of processing infectious agents. Safety measures were carried out by the team by providing guidelines, engineering controls, management policies and work practices. We followed a high standard of safe and responsible biological engineering. It is one of the primary concerns of the team and we ensured that all the works carried are highly safe. In general, iGEM teams are trying to pursue ambitious projects and reduce risks by using safer substitutes for more dangerous organisms/parts.

Project Safety

Our project which focuses on the production of minicins in which we mainly target gastro intestinal infections and biofilm formation though are mostly caused by hazardous organisms such as Shigella, Clostridium etc., Our team worked on class 2 organisms such as Pseudomonas aeruginosa and Staphylococcus aureus as model organism to target the same with reduced risks and have a safe project design .

ORGANISMRISK GROUP
E. coli BL211
Pseudomonas aeruginosa2
Staphylococcus aureus2

As our college is provided with well equipped class1 and class 2 cabinets ,which made it easier to handle these organisms. Proper bio safety guidelines were also considered for cloning and synthetic biology works .

Lab Safety

We the team members have taken special online Good Microbiological Laboratory Practices before the project was initiated. Though we reduced the risk hazards of the project by working on class 1 & class 2, safety is essential all the time. The lab team concentrates more on the safety of handling organisms and parts used in the project. All the safety cabinets and labs used for the project are well furnished with separate rooms for the cabinets . Safety regarding chemicals used in the project is also considered, especially for Etbr, agarose and other carcinogens which were always handled with gloves. The containment is also well autoclaved and discarded by proper disposables to prevent risks to the environment . All the safety measures followed by the team were guided by our instructors and we have also ensured that the final product of the work produces no risk or reduced risk to the environment and other living organisms.

Lets talk!

Sri Venkateswara College of Engineering, Chennai