Difference between revisions of "Team:SVCE Chennai/Safety"

 
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        <h2 class="titleStyle">Safety</h2>
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            <h5>Table of Contents</h5><hr class="no-margin">
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            <ul class="list-unstyled">
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                <li>
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                    <a href="#intro">
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                        <span class="fa fa-angle-double-right text-primary"></span>Introduction
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                <li>
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                    <a href="#projsafety">
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                        <span class="fa fa-angle-double-right text-primary"></span>Project Safety
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                    <a href="#labsafety">
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                        <span class="fa fa-angle-double-right text-primary"></span>Lab Safety
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        <h3 id="intro" class="title">Introduction</h3>
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        <p>Laboratories are often recognized with hazards of processing infectious agents. Safety measures were
  
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carried out by the team by providing guidelines, engineering controls, management policies and work
  
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practices. We followed a high standard of safe and responsible biological engineering. It is one of the primary
  
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concerns of the team and we ensured that all the works carried are highly safe. In general, iGEM teams are trying
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            <p class="lead"><img src="https://static.igem.org/mediawiki/2015/7/74/Aalto-Helsinki_black_logo_text.png" style="width:75%;height:75%"></img> </p>
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            to pursue ambitious projects and reduce risks by using safer substitutes for more dangerous organisms/parts.</p>
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        <h3 id="projsafety" class="title">Project Safety</h3>
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        <p>Our project which focuses on the production of minicins in which we mainly target gastro intestinal infections and
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                <li><a href="https://2015.igem.org/Team:Aalto-Helsinki">Project</a>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Background">Background</a></li>
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                      <li><a href="#">Cellulose</a></li>
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                      <li><a href="#">Propane</a></li>
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                      <li><a href="#">Micelle</a></li>
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                <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling">Modeling</a>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Kinetics">Kinetics</a></li>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_propane">Models of propane pathway</a></li>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_synergy">Synergy model</a></li>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_micelle">Micelle model</a></li>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_cellulose">About cellulose pathway</a></li>
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                <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Lab" >Lab</a>
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                <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Lab" >Results</a>
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                      <li><a href="#">Medal criteria</a></li>
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                <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Practices">Practices</a>
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                      <li><a href="#">Outreach</a></li>
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                <li><a href="#">Community</a>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Collaborations">Collaboration</a></li>
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                      <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Attributions">Attributions</a></li>
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                      <li><a href="#">Acknowledgements</a></li>
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                  </ul>
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                </li>
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                <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Journal">Journal</a>
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                </li>
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                <li><a href="#">Future</a>
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            </ul>
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          </div>
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biofilm formation though are mostly caused by hazardous organisms such as Shigella, Clostridium etc., Our
  
 +
team worked on class 2 organisms such as Pseudomonas aeruginosa and Staphylococcus aureus as model
  
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organism to target the same with reduced risks and have a safe project design .</p>
 +
       
 +
       
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<table class="table table-bordered">
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        <thead><tr><th>ORGANISM</th><th>RISK GROUP</th></tr></thead>
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    <tbody>
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        <tr><td>E. coli BL21</td><td>1</td></tr>
 +
        <tr><td>Pseudomonas aeruginosa</td><td>2</td></tr>
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        <tr><td>Staphylococcus aureus</td><td>2</td></tr>
 +
     
 +
    </tbody></table>
 +
       
 +
        <p>As our college is provided with well equipped class1 and class 2 cabinets ,which made it easier to handle these
  
−
+
organisms. Proper bio safety guidelines were also considered for cloning and synthetic biology works .</p>
 +
       
 +
        <h3 id="labsafety" class="title">Lab Safety</h3>
 +
       
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        <p style="padding-bottom:100px;">We the team members have taken special online Good Microbiological Laboratory Practices before the project
  
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was initiated. Though we reduced the risk hazards of the project by working on class 1 & class 2, safety is
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essential all the time. The lab team concentrates more on the safety of handling organisms and parts used in the
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<h2> Fuel for the Future </h2>
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<p>Climate change is argued to be one of the greatest challenges faced by mankind. The current climate change is mainly caused by us humans as we have been using the Earth’s precious fossil fuel stocks without returning the emitted gases into the natural carbon cycle. According to <a href="http://www.ipcc.ch/publications_and_data/ar4/wg1/en/ch10s10-7.html#10-7-1">IPCC</a>, even if we could stop all the emissions right now, the Earth’s average temperature would rise 0.6C. This means we must act now. To fight climate change we have come up with a solution which would tackle the emissions made by the road transportation. These emissions make up <a href="http://www.oecd.org/env/39762914.pdf">a considerable 11%</a> of the world’s greenhouse gas emissions.<br /><br />
+
  
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Imagine if you could drive your car knowing you won’t be contributing to the climate change. The carbon footprint of transported goods would diminish. What if we lived in a world without geo-political pressure caused by the unbalanced localization of usable fuels?<br /><br />
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project. All the safety cabinets and labs used for the project are well furnished with separate rooms for the
  
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Here’s our solution: we will produce sustainable propane in E. coli using cellulose as a carbon source. Propane is already common in car engines, and gasoline engines can be converted into propane engines quite simply and inexpensively. As propane is already widely used, the infrastructure to distribute propane is largely in place. But, the problem is that propane is currently produced as a side product of the petrochemical industry, hence it’s a fossil fuel. To solve this, we will transfer a propane producing pathway into E. coli. <a href="http://www.nature.com/ncomms/2014/140902/ncomms5731/full/ncomms5731.html">The pathway</a> is a patchwork of different enzymes from different organisms, but has been shown to work in E. coli alone. To elevate our propane producing E. coli from a glucose-consuming first generation biofuel to the second generation, we are integrating a secretion system for cellulose hydrolysing enzymes. This way our bacteria could survive on agricultural and food waste, closing the carbon cycle without disturbing the current food production. </p>
+
cabinets . Safety regarding chemicals used in the project is also considered, especially for Etbr, agarose and
  
 +
other carcinogens which were always handled with gloves. The containment is also well autoclaved and discarded
  
 +
by proper disposables to prevent risks to the environment . All the safety measures followed by the team were
  
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guided by our instructors and we have also ensured that the final product of the work produces no risk or reduced
  
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Latest revision as of 03:21, 19 September 2015

Safety

Introduction

Laboratories are often recognized with hazards of processing infectious agents. Safety measures were carried out by the team by providing guidelines, engineering controls, management policies and work practices. We followed a high standard of safe and responsible biological engineering. It is one of the primary concerns of the team and we ensured that all the works carried are highly safe. In general, iGEM teams are trying to pursue ambitious projects and reduce risks by using safer substitutes for more dangerous organisms/parts.

Project Safety

Our project which focuses on the production of minicins in which we mainly target gastro intestinal infections and biofilm formation though are mostly caused by hazardous organisms such as Shigella, Clostridium etc., Our team worked on class 2 organisms such as Pseudomonas aeruginosa and Staphylococcus aureus as model organism to target the same with reduced risks and have a safe project design .

ORGANISMRISK GROUP
E. coli BL211
Pseudomonas aeruginosa2
Staphylococcus aureus2

As our college is provided with well equipped class1 and class 2 cabinets ,which made it easier to handle these organisms. Proper bio safety guidelines were also considered for cloning and synthetic biology works .

Lab Safety

We the team members have taken special online Good Microbiological Laboratory Practices before the project was initiated. Though we reduced the risk hazards of the project by working on class 1 & class 2, safety is essential all the time. The lab team concentrates more on the safety of handling organisms and parts used in the project. All the safety cabinets and labs used for the project are well furnished with separate rooms for the cabinets . Safety regarding chemicals used in the project is also considered, especially for Etbr, agarose and other carcinogens which were always handled with gloves. The containment is also well autoclaved and discarded by proper disposables to prevent risks to the environment . All the safety measures followed by the team were guided by our instructors and we have also ensured that the final product of the work produces no risk or reduced risk to the environment and other living organisms.

Lets talk!

Sri Venkateswara College of Engineering, Chennai