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             <li><a href="https://static.igem.org/mediawiki/2015/f/fb/Yale_iGEM_Project_Summary_2015.pdf" alt="PDF Summary">PDF Summary</a></li>
 
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       <h2 id="overview">Collaborations</h2>
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       <h2 id="overview" class="text-center">Collaborations</h2>
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      <h3>Featuring University of La Verne and the University of Michigan!</h3>
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      <div class="page__button"><a href="#guidebook" class="custom__button">Guidebook</a><a href="#protocat" class="custom__button">ProtoCat</a>
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       <h2 id="guidebook">Developing a Guidebook - <a href="#" target="_blank" class="uni__link">University of La Verne iGEM</a></h2>
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       <h2 id="guidebook">Beginning Research in Non-Model Microbes with <a href="https://2015.igem.org/Team:Concordia" target="https://2015.igem.org/Team:Concordia" class="uni__link">Concordia University iGEM</a>, <a href="https://2015.igem.org/Team:Cornell" target="https://2015.igem.org/Team:Cornell" class="uni__link">Cornell University iGEM</a>, <a href="https://2015.igem.org/Team:Northeastern_Boston" target="https://2015.igem.org/Team:Northeastern_Boston" class="uni__link">Northeastern University iGEM</a>, <a href="https://2015.igem.org/Team:British_Columbia" target="https://2015.igem.org/Team:British_Columbia" class="uni__link">University of British Columbia iGEM</a>, <a href="https://2015.igem.org/Team:LaVerne-Leos" target="https://2015.igem.org/Team:LaVerne-Leos" class="uni__link">University of La Verne iGEM</a>, and <a href="https://2015.igem.org/Team:Utah_State" target="https://2015.igem.org/Team:Utah_State" class="uni__link">Utah State University iGEM</a></h2>
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      <h3>Beginning Research in Non-Model Microbes</h3>
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       <p>Over the past few years, iGEM has increasingly centered around non-model microorganisms—organisms which are less well-characterized and have fewer resources for genetic manipulation than the model E. coli and S. cerevisiae. Despite a prevalence of non-model organisms in iGEM competition projects and the potential impact which these projects may have, few resources exist for teams hoping to initiate research in non-model strains.</p>
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      <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
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       <p>The 2015 Yale University iGEM team has collaborated with several other teams working in non-model strains to design a set of considerations for future iGEM teams in order to reduce the barrier to entry into non-model organisms. We synthesized the experiences of other teams into a handout, which has been made available on our team wiki and will be available at out poster at the Jamboree.</p>
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       <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
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      <p class="text-center"><a href="https://static.igem.org/mediawiki/2015/e/e9/Collaboration_Brochure_FINAL.pdf" class="file__link">2015 Non-Model Organism Handout!</a></p>
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       <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
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      <h2 id="protocat">ProtoCat - <a href="#" target="_blank" class="uni__link">University of Michigan</a></h2>
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      <h3>A Centralized, Validated Protocols Database</h3>
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        <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
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        <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
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            <h2>Yale iGem 2015</h2>
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            <p>Main Campus:</p>
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            <p>Yale Department of Molecular, Cellular & Developmental Biology</p>
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            <p>Attn: Farren Isaacs/iGEM</p>
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            <p>219 Prospect St</p>
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            <p>PO Box 208103</p>
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            <p>New Haven, CT 06520</p>
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            <p>Tel. +1(203) 432-3783</p>
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            <p>E-mail: igem.yale@gmail.com</p>
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          <p class="text-center">&copy; Yale iGEM 2015</p>
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Latest revision as of 03:29, 19 September 2015


<!DOCTYPE html> Yale iGem 2015: Collaborations

Collaborations

Beginning Research in Non-Model Microbes with Concordia University iGEM, Cornell University iGEM, Northeastern University iGEM, University of British Columbia iGEM, University of La Verne iGEM, and Utah State University iGEM

Over the past few years, iGEM has increasingly centered around non-model microorganisms—organisms which are less well-characterized and have fewer resources for genetic manipulation than the model E. coli and S. cerevisiae. Despite a prevalence of non-model organisms in iGEM competition projects and the potential impact which these projects may have, few resources exist for teams hoping to initiate research in non-model strains.

The 2015 Yale University iGEM team has collaborated with several other teams working in non-model strains to design a set of considerations for future iGEM teams in order to reduce the barrier to entry into non-model organisms. We synthesized the experiences of other teams into a handout, which has been made available on our team wiki and will be available at out poster at the Jamboree.

2015 Non-Model Organism Handout!

Yale iGEM 2015

Main Campus:

Yale Department of Molecular, Cellular & Developmental Biology

Attn: Farren Isaacs/iGEM

219 Prospect St

PO Box 208103

New Haven, CT 06520

Tel. +1(203) 432-3783

E-mail: igem.yale@gmail.com

© Yale iGEM 2015