Difference between revisions of "Team:UIUC Illinois/Composite Part"

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<h2> Composite Parts</h2>
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<h2> Part Documentation</h2>
  
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<ul>
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<h4>Note</h4>
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<img src="https://static.igem.org/mediawiki/2015/thumb/0/00/UIUC_Illinois_PartB_Updated.png/797px-UIUC_Illinois_PartB_Updated.png">
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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<li>Part: K1681000</li>
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<li>Part Name: PL lac O-1 SCRIBE(kanR)ON</li>
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<li>Part Type: n/a</li>
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<li>Creator: UIUC_Illinois_2015</li>
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<li>Short Description: IPTG-inducible kanamycin resistance SCRIBE cassette</li>
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<li>Long Description:
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SCRIBE(kanR)ON is a cassette coding for the RNA and proteins needed to restore kanamycin resistance by synthesizing multicopy single-stranded DNA (msDNA) for site-specific recombination.  The part consists of the IPTG-inducible promoter PL lac O-1, a retron coding for an untranslated RNA molecule and the open reading frame for reverse transcriptase, a RBS, the open reading frame for beta recombinase, and a terminator. The reverse transcriptase uses the msDNA (msd) region of the RNA molecule as a template and the msd RNA (msr) region of the RNA molecule as a primer to synthesize the msDNA. The beta recombinase inserts the kanamycin resistance segment of the msDNA into the host’s genome during DNA replication as an Okazaki fragment.</li>
  
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<p>
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<li>Design Considerations:
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters and/ or custom DNA segments for site-specific recombination.</li>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<img src="https://static.igem.org/mediawiki/2015/thumb/f/f3/UIUC_Illinois_PartA_Updated.png/800px-UIUC_Illinois_PartA_Updated.png">
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<li>Part: K1681001</li>
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<li>Part Name: SCRIBE(BsaI)</li>
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<li>Part Type: n/a</li>
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<li>Creator: UIUC_Illinois_2015</li>
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<li>Short Description: SCRIBE cassette with BsaI sites for Golden Gate assembly</li>
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<li>Long Description:
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SCRIBE(BsaI) is a SCRIBE cassette including two restriction sites of the Type-II restriction enzyme BsaI in the msd region of the retron. This part is compatible with Golden Gate assembly.</li>
  
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</div>
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<li>Design Considerations:
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This part was designed as a modular assembly part for future users to add custom DNA segments into the msd region for site-specific recombination via Golden Gate assembly and to add promoters of their choice via Standard or 3A assembly.
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See Bba_K1681000 for a more detailed description as to how such custom DNA segments would be inserted into the host’s genome.</li>
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</ul>
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<img src="https://static.igem.org/mediawiki/2015/thumb/9/95/UIUC_Illinois_PartD_Updated.png/800px-UIUC_Illinois_PartD_Updated.png">
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<li>K1681002</li>
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<li>Part Name: PL lac O-1 SCRIBE(BsaI)</li>
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<li>Part Type: n/a</li>
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<li>Creator: UIUC_Illinois_2015</li>
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<li>Short Description: IPTG-inducible promoter and BsaI SCRIBE cassette</li>
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<li>Long Description:
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This part is the IPTG-inducible promoter PL lac O-1 immediately upstream of SCRIBE(BsaI), with no scar site in between.</li>
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<li>Design Considerations:
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This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters after using Golden Gate assembly to add custom DNA segments into the msd region for site-specific recombination.
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See Bba_K1681000 for a more detailed description as to how such custom DNA segments would be inserted into the host’s genome.</li>
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</ul>
  
 
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Latest revision as of 03:31, 19 September 2015

Part Documentation

  • Part: K1681000
  • Part Name: PL lac O-1 SCRIBE(kanR)ON
  • Part Type: n/a
  • Creator: UIUC_Illinois_2015
  • Short Description: IPTG-inducible kanamycin resistance SCRIBE cassette
  • Long Description: SCRIBE(kanR)ON is a cassette coding for the RNA and proteins needed to restore kanamycin resistance by synthesizing multicopy single-stranded DNA (msDNA) for site-specific recombination. The part consists of the IPTG-inducible promoter PL lac O-1, a retron coding for an untranslated RNA molecule and the open reading frame for reverse transcriptase, a RBS, the open reading frame for beta recombinase, and a terminator. The reverse transcriptase uses the msDNA (msd) region of the RNA molecule as a template and the msd RNA (msr) region of the RNA molecule as a primer to synthesize the msDNA. The beta recombinase inserts the kanamycin resistance segment of the msDNA into the host’s genome during DNA replication as an Okazaki fragment.
  • Design Considerations: This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters and/ or custom DNA segments for site-specific recombination.
  • Part: K1681001
  • Part Name: SCRIBE(BsaI)
  • Part Type: n/a
  • Creator: UIUC_Illinois_2015
  • Short Description: SCRIBE cassette with BsaI sites for Golden Gate assembly
  • Long Description: SCRIBE(BsaI) is a SCRIBE cassette including two restriction sites of the Type-II restriction enzyme BsaI in the msd region of the retron. This part is compatible with Golden Gate assembly.
  • Design Considerations: This part was designed as a modular assembly part for future users to add custom DNA segments into the msd region for site-specific recombination via Golden Gate assembly and to add promoters of their choice via Standard or 3A assembly. See Bba_K1681000 for a more detailed description as to how such custom DNA segments would be inserted into the host’s genome.
  • K1681002
  • Part Name: PL lac O-1 SCRIBE(BsaI)
  • Part Type: n/a
  • Creator: UIUC_Illinois_2015
  • Short Description: IPTG-inducible promoter and BsaI SCRIBE cassette
  • Long Description: This part is the IPTG-inducible promoter PL lac O-1 immediately upstream of SCRIBE(BsaI), with no scar site in between.
  • Design Considerations: This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters after using Golden Gate assembly to add custom DNA segments into the msd region for site-specific recombination. See Bba_K1681000 for a more detailed description as to how such custom DNA segments would be inserted into the host’s genome.