Difference between revisions of "Team:UIUC Illinois/Results"
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| − | <h2> | + | <h2>Results:</h2> |
| + | <p>Despite encountering high levels of mutations throughout our various attempts to make a standardized version of the SCRIBE system, on the final day before our parts were due, we finally saw signs of successful recombination in response to IPTG! </p> | ||
| + | <p>Results that constitute a characterization of our part can be found on its registry page, located <a href="http://parts.igem.org/Part:BBa_K1681000">here.</a><p> | ||
| + | <img style="float:right;height:200px;width:300px;" src="https://static.igem.org/mediawiki/2015/7/72/Successuiuc.jpeg" /> | ||
| + | <h2>Unsuccessful Results:</h2> | ||
| + | <p>We were unable to obtain a functional construct of SCRIBE without the lac promoter</p> | ||
| − | <p> | + | <p>Because of this we were unable to carry out our original goal of examining SCRIBE's potential biosensing applications</p> |
| − | < | + | <h2>Future plans:</h2> |
| − | + | <p>Add other promoters upstream of the SCRIBE cassette to expand its possible applications, particularly in environmental biosensing applications, such as groundwater contamination.</p> | |
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| + | <p>Combine SCRIBE with the genome-editing tools to function in eukaryotic organisms</p> | ||
| + | <p>Further examine potential mutagenic effects related to the overproduction of msDNA and the impact it may have had on our project [1].</p> | ||
| − | + | <p>[1] Mao JR1, Inouye S, Inouye M. "Enhancement of frame-shift mutation by the overproduction of msDNA in Escherichia coli." FEMS Microbiol Lett. 1996 Oct 15;144(1):109-15.</p> | |
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Latest revision as of 03:44, 19 September 2015
Results:
Despite encountering high levels of mutations throughout our various attempts to make a standardized version of the SCRIBE system, on the final day before our parts were due, we finally saw signs of successful recombination in response to IPTG!
Results that constitute a characterization of our part can be found on its registry page, located here.
Unsuccessful Results:
We were unable to obtain a functional construct of SCRIBE without the lac promoter
Because of this we were unable to carry out our original goal of examining SCRIBE's potential biosensing applications
Future plans:
Add other promoters upstream of the SCRIBE cassette to expand its possible applications, particularly in environmental biosensing applications, such as groundwater contamination.
Combine SCRIBE with the genome-editing tools to function in eukaryotic organisms
Further examine potential mutagenic effects related to the overproduction of msDNA and the impact it may have had on our project [1].
[1] Mao JR1, Inouye S, Inouye M. "Enhancement of frame-shift mutation by the overproduction of msDNA in Escherichia coli." FEMS Microbiol Lett. 1996 Oct 15;144(1):109-15.