Difference between revisions of "Team:Lethbridge HS/Results"
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+ | document.getElementById("projectText1").write('The purpose of hospitals is to help people get better. However, in the United States, 2 million people are infected during their hospital stay and bacterial biofilms are responsible of 65% of all hospital acquired infections. A biofilm is a conglomeration of bacteria that is enclosed in a matrix of sugars and extracellular DNA, this helps to hold the bacteria together like'); | ||
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+ | jQuery("#coloredTitle").text('Varroa'); | ||
+ | jQuery("#slide1Title").text('Introduction'); | ||
+ | jQuery("#slide1Paragraph").text('The top 100 food crops produced provide 90% of the world’s nutrition. 70% of these crops are pollinated by bees. A phenomenon called Colony Collapse Disorder (CCD) has decimated honeybee colonies across the world, halving the number of productive colonies worldwide. One of the main factors hypothesized to contribute to CCD is the mite and viral vector Varroa destructor. While feeding on the bee’s hemolymph, Varroa destructor expels RNA viruses into the bee crippling colony’s strength. Current commercial methods to eradicate Varroa have seen a gradual development of resistance in treated populations. Using synthetic biology, we plan to target Varroa more effectively by directly delivering the miticide, oxalic acid into Varroa and utilizing RNA interference to eliminate Varroa populations within commercial hives.'); | ||
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− | < | + | <body class="body_human_practices"> |
− | < | + | <nav class="navbar navbar-inverse navbar-fixed-top menu" id=""> |
− | < | + | <div class="container-fluid"> |
− | </ | + | |
+ | <!--Lethbridge HS iGEM Logo --> | ||
+ | <div class="navbar-header"> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Introduction" id="nav-head" class="navbar-brand" style="margin-top:10px;"><h1 id="headerText" style="font-weight:100; font-family: 'existence'; ">Lethbridge iGEM </h1></a> | ||
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+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Description">Background</a> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Experiments">Experiments</a> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Results">Results</a> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Parts">Parts</a> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Achievements">Achievements</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="texItem" style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Practices">Human Practices</a></li> | ||
+ | <li margin-top:2%; style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Notebook">Notebook</a></li> | ||
+ | <li margin-top:2%; style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Safety">Safety</a></li> | ||
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+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Team">Students</a> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Team#section2">Advisors</a> | ||
+ | <a class="texItem" href="https://2015.igem.org/Team:Lethbridge_HS/Team#section3">Sponsors</a> | ||
+ | <a class="texItem" href="https://2015.igem.org/Team:Lethbridge_HS/Attributions">Attributions</a> | ||
+ | <a class="texItem" href="https://2015.igem.org/Team:Lethbridge_HS/Collaborations">Collaborations</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class=""><a class="picItem" href="https://2015.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2015/2/21/LethHS2015_igemlogo.png" width="50px" height="45px" style="margin-top:-9%; margin-bottom:-10%;"></a></li> | ||
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+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | |||
+ | <div class="jumbotron" id="titleSlide_hp" style="background-image:url('https://static.igem.org/mediawiki/2015/3/37/LethHS2015_honeycomb.jpg');"> | ||
− | < | + | <div class="flaticon-dna9 pageIcon"></div> |
− | <p> | + | <p id="pageTitleText">Results<br></p><p id="pageSubtitleText"><span>What results did we get in the lab?</span></p> |
− | <ul> | + | </div> |
− | <li><a href=" | + | |
− | <li><a href=" | + | <div class="container-fluid" id="regularPageBody" style="padding-left:10%; padding-right:10%;"> |
− | <li><a href=" | + | <div class="row"> |
− | </ul> | + | <div class="col-md-3" id="mainBodySideBar" style="display:none;"> |
+ | <div class="sideBarContent"> | ||
+ | <ul> | ||
+ | <li><a href="#section1"><h2>Description</h2></a></li> | ||
+ | <li class="biofilms"><a href="#section1"><p>What is nuclease? What is dextranase?</p></a></li> | ||
+ | <li class="biofilms"><a href="#section1"><p>What we are doing differently</p></a></li> | ||
+ | <li class="biofilms"><a href="#section1"><p>Extracellular Polymeric Substance Matrix</p></a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-12 col-sm-12"> | ||
+ | <section id="section1"> | ||
+ | </section> | ||
+ | <h1 id="projecttext1" class="contentSubTitle"> Results <br><small></small></h1> | ||
+ | <p id="humanpractices_hp" class="bees"><b>Oxalic acid results:</b><br></p> | ||
+ | |||
+ | <div style="width:60%;" class="bees"> | ||
+ | <table class="table table-striped table-bordered"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Oxalic Acid Concentration (mg/mL)</th> | ||
+ | <th>OD528</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>0.1</td> | ||
+ | <td>1.862</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.2</td> | ||
+ | <td>1.675</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.3</td> | ||
+ | <td>1.675</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.4</td> | ||
+ | <td>1.655</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.5</td> | ||
+ | <td>1.673</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.6</td> | ||
+ | <td>1.680</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.7</td> | ||
+ | <td>1.655</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.8</td> | ||
+ | <td>1.630</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.9</td> | ||
+ | <td>1.587</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>1.0</td> | ||
+ | <td>1.564</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
</div> | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2015/c/c9/LethHS2015_oxalicacidcurve.jpg" style="" width="600px" height="400px" class="bees img-responsive"> | ||
+ | |||
+ | <p id="humanpractices_hp" class="bees"><b>Bee trials:</b><br></p> | ||
+ | |||
+ | |||
+ | <table class="table table-striped table-bordered bees"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Treatment</th> | ||
+ | <th>Before</th> | ||
+ | <th>After</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>None</td> | ||
+ | <td>Somewhat lethargic. Drank when prompted.</td> | ||
+ | <td>Appeared tired, but otherwise unaffected.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.00%</td> | ||
+ | <td>Most lethargic, did not eat when offered</td> | ||
+ | <td>Wet and slow moving.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.05%</td> | ||
+ | <td>Very vigorous; ran around inside the tube. Fed.</td> | ||
+ | <td>Very energetic. Unaffected by treatment.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.2%</td> | ||
+ | <td>Very vigorous; ran around inside the tube. Fed.</td> | ||
+ | <td>Slightly less energetic than the 0.05% bee.</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="bees">The bees will be able to survive all of the oxalic acid that the E.coli can produce.</p> | ||
+ | <p id="humanpractices_hp" class="bees"><b>Mite trials:</b><br></p> | ||
+ | <table class="table table-striped table-bordered bees"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Control</th> | ||
+ | <th>Oxalic Acid</th> | ||
+ | <th>RFP</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td><b>Parafilm Pouch</b></td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>Larvae 1</b></td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>Larvae 2</b></td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="bees">The mites did not survive any of the trials.</p> | ||
+ | |||
+ | |||
+ | <p id="humanpractices_hp" class="biofilms"><b>Antibiotics testing:</b><br></p> | ||
+ | <table class="table table-striped table-bordered biofilms"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Test (Antibiotics)</th> | ||
+ | <th>Result</th> | ||
+ | <th>After</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>AMP 1</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>AMP 2</td> | ||
+ | <td>Positive</td> | ||
+ | <td>Wet and slow moving.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CAM 1</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CAM 2</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>TET 1</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> <tr> | ||
+ | <td>TET 2</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Control (water)</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="biofilms">The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.</p> | ||
+ | |||
+ | <p class="biofilms"><b>Testing with cleaners</b></p> | ||
+ | <table class="table table-striped table-bordered biofilms"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Cleaner</th> | ||
+ | <th>Results</th> | ||
+ | <th>After</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>Bleach</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Tilex</td> | ||
+ | <td>3</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Mr. Clean</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Fantastik</td> | ||
+ | <td>1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Truly</td> | ||
+ | <td>2</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Windex</td> | ||
+ | <td>1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Lysol</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>GreenWorks</td> | ||
+ | <td>1</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="biofilms"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Biofilms fluorescent imaging under Typhoon</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/1/1c/LethHS2015_6.jpg" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Different concentration of oxalic acid 3 minutes into incubation time at RT</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/4/48/LethHS2015_7.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/c/c3/LethHS2015_8.jpg" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/f/fd/LethHS201510.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Microcentrifuge tube containing Honey Bee</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/d/d3/LethHS201511.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Microcentrifuge tube containing Varroa mites</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <script src="https://2015.igem.org/Team:Lethbridge_HS/Animations_JS?action=raw&ctype=text/javascript"></script> | ||
+ | </body> | ||
</html> | </html> |
Latest revision as of 03:46, 19 September 2015
Results
What results did we get in the lab?
Results
Oxalic acid results:
Oxalic Acid Concentration (mg/mL) | OD528 |
---|---|
0.1 | 1.862 |
0.2 | 1.675 |
0.3 | 1.675 |
0.4 | 1.655 |
0.5 | 1.673 |
0.6 | 1.680 |
0.7 | 1.655 |
0.8 | 1.630 |
0.9 | 1.587 |
1.0 | 1.564 |
Bee trials:
Treatment | Before | After |
---|---|---|
None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
Control | Oxalic Acid | RFP | |
---|---|---|---|
Parafilm Pouch | Mites dead | Mites dead | Mites dead |
Larvae 1 | Mites dead | Mites dead | Mites dead |
Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
Test (Antibiotics) | Result | After |
---|---|---|
AMP 1 | Positive | |
AMP 2 | Positive | Wet and slow moving. |
CAM 1 | Positive | |
CAM 2 | Positive | |
TET 1 | Positive | |
TET 2 | Positive | |
Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
Testing with cleaners
Cleaner | Results | After |
---|---|---|
Bleach | - | |
Tilex | 3 | |
Mr. Clean | - | |
Fantastik | 1 | |
Truly | 2 | |
Windex | 1 | |
Lysol | - | |
GreenWorks | 1 |