Difference between revisions of "Team:Lethbridge HS/Results"
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− | <a href="https://2015.igem.org/Team:Lethbridge_HS/Description"> | + | <a href="https://2015.igem.org/Team:Lethbridge_HS/Description">Background</a> |
− | <a href=" | + | <a href="https://2015.igem.org/Team:Lethbridge_HS/Experiments">Experiments</a> |
− | <a href=" | + | <a href="https://2015.igem.org/Team:Lethbridge_HS/Results">Results</a> |
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Parts">Parts</a> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge_HS/Achievements">Achievements</a> | ||
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<li margin-top:2%; style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Notebook">Notebook</a></li> | <li margin-top:2%; style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Notebook">Notebook</a></li> | ||
<li margin-top:2%; style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Safety">Safety</a></li> | <li margin-top:2%; style="margin-top:2%;"><a class="textItem" href="https://2015.igem.org/Team:Lethbridge_HS/Safety">Safety</a></li> | ||
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<a href="https://2015.igem.org/Team:Lethbridge_HS/Team">Students</a> | <a href="https://2015.igem.org/Team:Lethbridge_HS/Team">Students</a> | ||
− | <a href="#">Advisors</a> | + | <a href="https://2015.igem.org/Team:Lethbridge_HS/Team#section2">Advisors</a> |
− | <a class="texItem" href="#">Sponsors</a> | + | <a class="texItem" href="https://2015.igem.org/Team:Lethbridge_HS/Team#section3">Sponsors</a> |
− | <a class="texItem" href=" | + | <a class="texItem" href="https://2015.igem.org/Team:Lethbridge_HS/Attributions">Attributions</a> |
− | <a class="texItem" href=" | + | <a class="texItem" href="https://2015.igem.org/Team:Lethbridge_HS/Collaborations">Collaborations</a> |
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− | <p id="pageTitleText"> | + | <p id="pageTitleText">Results<br></p><p id="pageSubtitleText"><span>What results did we get in the lab?</span></p> |
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− | <img src="https://static.igem.org/mediawiki/2015/ | + | <h1 id="projecttext1" class="contentSubTitle"> Results <br><small></small></h1> |
− | <img src="https://static.igem.org/mediawiki/2015/c/ | + | |
+ | <p id="humanpractices_hp" class="bees"><b>Oxalic acid results:</b><br></p> | ||
+ | |||
+ | <div style="width:60%;" class="bees"> | ||
+ | <table class="table table-striped table-bordered"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Oxalic Acid Concentration (mg/mL)</th> | ||
+ | <th>OD528</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>0.1</td> | ||
+ | <td>1.862</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.2</td> | ||
+ | <td>1.675</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.3</td> | ||
+ | <td>1.675</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.4</td> | ||
+ | <td>1.655</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.5</td> | ||
+ | <td>1.673</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.6</td> | ||
+ | <td>1.680</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.7</td> | ||
+ | <td>1.655</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.8</td> | ||
+ | <td>1.630</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.9</td> | ||
+ | <td>1.587</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>1.0</td> | ||
+ | <td>1.564</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2015/c/c9/LethHS2015_oxalicacidcurve.jpg" style="" width="600px" height="400px" class="bees img-responsive"> | ||
+ | |||
+ | <p id="humanpractices_hp" class="bees"><b>Bee trials:</b><br></p> | ||
+ | |||
+ | |||
+ | <table class="table table-striped table-bordered bees"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Treatment</th> | ||
+ | <th>Before</th> | ||
+ | <th>After</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>None</td> | ||
+ | <td>Somewhat lethargic. Drank when prompted.</td> | ||
+ | <td>Appeared tired, but otherwise unaffected.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.00%</td> | ||
+ | <td>Most lethargic, did not eat when offered</td> | ||
+ | <td>Wet and slow moving.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.05%</td> | ||
+ | <td>Very vigorous; ran around inside the tube. Fed.</td> | ||
+ | <td>Very energetic. Unaffected by treatment.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.2%</td> | ||
+ | <td>Very vigorous; ran around inside the tube. Fed.</td> | ||
+ | <td>Slightly less energetic than the 0.05% bee.</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="bees">The bees will be able to survive all of the oxalic acid that the E.coli can produce.</p> | ||
+ | <p id="humanpractices_hp" class="bees"><b>Mite trials:</b><br></p> | ||
+ | <table class="table table-striped table-bordered bees"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Control</th> | ||
+ | <th>Oxalic Acid</th> | ||
+ | <th>RFP</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td><b>Parafilm Pouch</b></td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>Larvae 1</b></td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>Larvae 2</b></td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | <td>Mites dead</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="bees">The mites did not survive any of the trials.</p> | ||
+ | |||
+ | |||
+ | <p id="humanpractices_hp" class="biofilms"><b>Antibiotics testing:</b><br></p> | ||
+ | <table class="table table-striped table-bordered biofilms"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Test (Antibiotics)</th> | ||
+ | <th>Result</th> | ||
+ | <th>After</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>AMP 1</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>AMP 2</td> | ||
+ | <td>Positive</td> | ||
+ | <td>Wet and slow moving.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CAM 1</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>CAM 2</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>TET 1</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> <tr> | ||
+ | <td>TET 2</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Control (water)</td> | ||
+ | <td>Positive</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="biofilms">The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.</p> | ||
+ | |||
+ | <p class="biofilms"><b>Testing with cleaners</b></p> | ||
+ | <table class="table table-striped table-bordered biofilms"> <caption></caption> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Cleaner</th> | ||
+ | <th>Results</th> | ||
+ | <th>After</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>Bleach</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Tilex</td> | ||
+ | <td>3</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Mr. Clean</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Fantastik</td> | ||
+ | <td>1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Truly</td> | ||
+ | <td>2</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Windex</td> | ||
+ | <td>1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Lysol</td> | ||
+ | <td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>GreenWorks</td> | ||
+ | <td>1</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="biofilms"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Biofilms fluorescent imaging under Typhoon</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/1/1c/LethHS2015_6.jpg" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Different concentration of oxalic acid 3 minutes into incubation time at RT</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/4/48/LethHS2015_7.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/c/c3/LethHS2015_8.jpg" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>E. coli cultures under varying concentration of oxalic acid</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/f/fd/LethHS201510.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Microcentrifuge tube containing Honey Bee</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <figure style=" margin-right:20px; float:left;" class="bees"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/d/d3/LethHS201511.png" width=400px height=300px class="img-responsive"> | ||
+ | <figcaption>Microcentrifuge tube containing Varroa mites</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | |||
+ | |||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 03:46, 19 September 2015
Results
What results did we get in the lab?
Results
Oxalic acid results:
Oxalic Acid Concentration (mg/mL) | OD528 |
---|---|
0.1 | 1.862 |
0.2 | 1.675 |
0.3 | 1.675 |
0.4 | 1.655 |
0.5 | 1.673 |
0.6 | 1.680 |
0.7 | 1.655 |
0.8 | 1.630 |
0.9 | 1.587 |
1.0 | 1.564 |
![](https://static.igem.org/mediawiki/2015/c/c9/LethHS2015_oxalicacidcurve.jpg)
Bee trials:
Treatment | Before | After |
---|---|---|
None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
Control | Oxalic Acid | RFP | |
---|---|---|---|
Parafilm Pouch | Mites dead | Mites dead | Mites dead |
Larvae 1 | Mites dead | Mites dead | Mites dead |
Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
Test (Antibiotics) | Result | After |
---|---|---|
AMP 1 | Positive | |
AMP 2 | Positive | Wet and slow moving. |
CAM 1 | Positive | |
CAM 2 | Positive | |
TET 1 | Positive | |
TET 2 | Positive | |
Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
Testing with cleaners
Cleaner | Results | After |
---|---|---|
Bleach | - | |
Tilex | 3 | |
Mr. Clean | - | |
Fantastik | 1 | |
Truly | 2 | |
Windex | 1 | |
Lysol | - | |
GreenWorks | 1 |
![](https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png)
![](https://static.igem.org/mediawiki/2015/1/1c/LethHS2015_6.jpg)
![](https://static.igem.org/mediawiki/2015/4/48/LethHS2015_7.png)
![](https://static.igem.org/mediawiki/2015/c/c3/LethHS2015_8.jpg)
![](https://static.igem.org/mediawiki/2015/f/fd/LethHS201510.png)
![](https://static.igem.org/mediawiki/2015/d/d3/LethHS201511.png)