Latest revision as of 03:52, 19 September 2015

The tour finishes here, but there is more to see. Follow the final link towards the end of our tour.

Attributions

General support

Institute for Complex Molecular Systems (ICMS) provided us with a room (including pet mouse).

Project Advisors

Prof. dr. ir. L. Brunsveld, dr. Ir. T.F.A. de Greef and dr. M. Merkx helped us out with brainstorming and troubleshooting.

Lab support

General lab support

Glenn Cremers, BSc

Glenn Cremers is our main support when we’re working in the lab. Because of his membership of last year’s team, he and his capacities can support us when difficulties come forward. Glenn is studying Biomedical Engineering at the Eindhoven University of Technology and just finished his bachelor. He will continue with his master at the department of chemical biology at the faculty of Biomedical Engineering. Assisting the team of 2015 is a chance to see more of the possibilities in this field of research.

Lab Stuff

Ir. R. Arts provided us with Phusion DNA polymerase and NanoGlo substrate.
Ir. S.J.A. Aper provided us with Kapa2G and DpnI.
Dr. S. Ludwig provided us with the T7 terminator primer.
Dr. S.F.R. Leysen provided us with the pETDuet1 vector.

Difficult technique support

M. van Rosmalen MSc.

Martijn van Rosmalen is a PhD student at Eindhoven University within the Merkx Group. In his research, he aims to apply yeast display and fluorescence activated cell sorting to the development of new FRET sensors. Martijn van Rosmalen maintains the FACS and took the time to familiarize us with the FACS.

gave us a helpful FACS introduction, helped us with questions during our experiments and sacrificed his day to fix the FACS to make sure we could use it.
Wiggert Altenburg

Wiggert Altenburg, BSc. is currently a first-years master student. Wiggert was a member of iGEM TU Eindhoven 2014 and in his role he did all the experiments with the FACS. In his bachelor thesis, Wiggert carried out and finetuned the Click Chemistry experiments which were presented at 2014's Giant Jamboree as Click Coli by iGEM TU Eindhoven.

assisted us with the first experiment.

Aptamer support

Dr. ir. B.M.G. Janssen

Dr. Ir. B.M.G. Janssen is a currently a Post-Doc at our university. His research focuses on increasing the specificity towards tumor cells in cancer treatment by using antibodies.

shared his aptamer experience with us.

Plate reader introduction

We got a platereader introduction from A. den Hamer.

A. den Hamer is a PhD student in the research group of Prof. L. Brunsveld. Her project involves the development of a 14-3-3 protein scaffold for the application of small molecule controlled signaling platforms used in a synthetic signaling network.

Complementary DNA support

Ir. W. Engelen

Ir. W. Engelen is a PhD student at our university. In the research group of dr. M. Merkx his project involves the development of novel diagnostic techniques for the sensitive and selective detection of antibodies in serum and saliva.

checked our complementary DNA design.

Biolumiscence assay support

Ir. R. Arts

Ir. R. Arts is a PhD student at our university. His main focus is the development of a modular & labor-friendly antibody detection protein which should provide a basis to develop antibody detection assays that are suitable for high-throughput screening and application in low-resource areas.

advised us on our bioluminiscence assay.

Safety support

GMO regulations at the TU/e

Dr. M. de Liefde - van Beest

Dr. M. de Liefde - van Beest is operating in the Department of Biomedical Engineering section Soft Tissue Biomechanics & Tissue engineering at our university. She is an expert in the field of biosafety.

is biosafety officer at our university and informed us about GMO regulations.

A tour through the lab

Ing. P.T.H.M. de Graaf - Heuvelmans

Ing. P.T.H.M. de Graaf - Heuvelmans is working at the department of Biomedical Engineering at our university. Her main responsibility is the lab.

gave us a lab tour and granted us lab access.

Policy Practices support

We were helped by many people for the Policy Practices part of our project. You can find a complete list of all the people who helped us out here.

Collaborations

Amoy newsletter

Thanks to iGEM Paris Bettencourt, Amoy & Pasteur for making this newsletter.

Cloning guide

Thanks to all teams who shared their knowledge with us and wrote a chapter in our cloning guide:

Traditional cloning:

Gibson Assembly:

Golden Gate Assembly:

Iterative Capped Assembly:

UCLA

3A Assembly:

UIUC Illinois

In-Fusion:

Toulouse

Ligation-Cycling Reaction:

CSU Fort Collins

Yeast Recombinant:

Minnesota

Wiki support

We built the wiki ourselves. For designing the website we used:

The Noun Project

The icons used in the menubar are obtained from thenounproject.com

"Home" icon by Vasu Adiga
"Team" icon by Björn Andersson
"Project" icon by Mister Pixel
"Results" icon by Simple Icons
"Policy Practices" icon by Rémy Médard
"Modeling" icon by Creative Stall
"Sponsors" icon by Marco Galtarossa
"Facebook" icon by Niels Gesquiere
"Twitter" icon by Luboš
"Collaborations" icon by Creative Stall
"Arrow up" icon by Riley Shaw
"Arrow down" icon by Riley Shaw
"Cloning Guide" icon by Joao Paulo
"Collaboration Attributions" by Marcio Duarte
"Safety" icon by OCHA visual information unit
"Report" by João Marcelo Ribeiro, BR
"Filter" by Anusha Narvekar, IN
"Arrow up" by P.J. Onori

The Aleo Font

The font we use on our wiki is designed by Alessio Laiso and released under the SIL Open Font License, which makes it free to use for both personal and commercial use. More info on the Aleo Font can be found here.

Miscellaneous

Easter Eggs

Iggy Azalea and Britney Spears for their contribution to the memory. (Yes, it's really there; try clicking on Hans' photo)
Tess Gaerthé for her contribution to the memory. (Click on Cas, her greatest fan; both literally and figuratively)
Yeshi de Bruin for her debut album "Yeshi Summer Mix"
Jaap Valkhoff and the Feijenoord legion for "Hand in hand, kameraden"
The M-Kids with their hitsong "SMS"
Tiger Love's "Pussy Cocaine" is a well appreciated addition to our memory, especially for Jan-Willem
The He-Man rendition of "What's Up", originally sung by 4 Non Blondes
50 Cents' "P.I.M.P."; the song of Kwankwan, our local G

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+<div id="wikiTour">
+<a style="text-decoration: none" href="https://2015.igem.org/Team:TU_Eindhoven/End_of_Tour">
+<div id="moreDepth">
+<span class="tekst1BI">Next Chapter</span><br />
+<img class="tourButton" src="https://static.igem.org/mediawiki/2015/7/7c/TU_Eindhoven_PlayButton.png">
+<span class="tekst1">
+The tour finishes here, but there is more to see. Follow the final link towards the end of our tour.
+</span>
+</div>
+</a>
+</div>
 <div id="containercontent">
+<div id="landingPage">
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@@ Line 16: / Line 30: @@
 <br /><br />
 <span class="tekst1">
-Institute for Complex Molecular Systems (ICMS) provided us with a room (including mouse).
+Institute for Complex Molecular Systems (ICMS) provided us with a room (including pet mouse).
 </span>
 <br />
@@ Line 32: / Line 46: @@
 <h4> Lab support</h4>
-<br /><br />
+<img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton13" class="spoilerbutton"><div class="spoiler" id="spoiler13">
 <h5> General lab support </h5>
 <br />
 <span class="tekst1">
 Glenn Cremers, BSc <img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton1" class="spoilerbutton"><div class="spoiler" id="spoiler1">
-Glenn Cremers is our main support when we’re working in the lab. Because of his membership of last year’s team, he and his capacities can support us when difficulties come forward. Glenn studies Biomedical Engineering at the Eindhoven University of Technology and just finished his bachelor. He will continue with his master at the department of chemical biology at the faculty of Biomedical Engineering. Assisting the team of 2015 is a chance to see more of the possibilities in this field of research.
+Glenn Cremers is our main support when we’re working in the lab. Because of his membership of last year’s team, he and his capacities can support us when difficulties come forward. Glenn is studying Biomedical Engineering at the Eindhoven University of Technology and just finished his bachelor. He will continue with his master at the department of chemical biology at the faculty of Biomedical Engineering. Assisting the team of 2015 is a chance to see more of the possibilities in this field of research.
 </div>
@@ Line 45: / Line 59: @@
 <br />
 <span class="tekst1">
-Ir. R. Arts provided us with Phusion DNA polymerase. <br />
+Ir. R. Arts provided us with Phusion DNA polymerase and NanoGlo substrate. <br />
 Ir. S.J.A. Aper provided us with Kapa2G and DpnI. <br />
-Dr. S. Ludwig provided us with the T7 terminator primer.
+Dr. S. Ludwig provided us with the T7 terminator primer.<br/>
+Dr. S.F.R. Leysen provided us with the pETDuet1 vector.
 </span>
 <br /><br />
@@ Line 58: / Line 73: @@
 Martijn van Rosmalen is a PhD student at Eindhoven University within the Merkx Group. In his research, he aims to apply yeast display and fluorescence activated cell sorting to the development of new FRET sensors. Martijn van Rosmalen maintains the FACS and took the time to familiarize us with the FACS.
 </div>
-gave us a helpful FACS introduction. <br />
+gave us a helpful FACS introduction, helped us with questions during our experiments and sacrificed his day to fix the FACS to make sure we could use it. <br />
 Wiggert Altenburg <img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton3" class="spoilerbutton"><div class="spoiler" id="spoiler3">
-Wiggert Altenburg is currently a first-years master student. Wiggert was a member of iGEM TU Eindhoven 2014 and in his role he did all the experiments with the FACS. In his bachelor thesis, Wiggert carried out and finetuned the Click Chemistry experiments which were presented at 2014's Giant Jamboree as Click Coli by iGEM TU Eindhoven.</div>
+Wiggert Altenburg, BSc. is currently a first-years master student. Wiggert was a member of iGEM TU Eindhoven 2014 and in his role he did all the experiments with the FACS. In his bachelor thesis, Wiggert carried out and finetuned the Click Chemistry experiments which were presented at 2014's Giant Jamboree as Click Coli by iGEM TU Eindhoven.</div>
 assisted us with the first experiment.
 </span>
@@ Line 88: / Line 103: @@
 <span class="tekst1">
 Ir. W. Engelen <img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton6" class="spoilerbutton"><div class="spoiler" id="spoiler6">
-Ir. W. Engelen is a PhD student at our university. In the research group of dr. M. Merkx his projects involves the development of novel diagnostic techniques for the sensitive and selective detection of antibodies in serum and saliva.
+Ir. W. Engelen is a PhD student at our university. In the research group of dr. M. Merkx his project involves the development of novel diagnostic techniques for the sensitive and selective detection of antibodies in serum and saliva.
 </div>
 checked our complementary DNA design.
@@ Line 98: / Line 113: @@
 <span class="tekst1">
 Ir. R. Arts <img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton7" class="spoilerbutton"><div class="spoiler" id="spoiler7">
-Ir. R. Arts is a PhD student at our university. His main focus is the development of a modular &amp; labor-friendly antibody detection protein. Which should provide a basis to develop antibody detection assays that are suitable for high-throughput screening and application in low-resource areas.
+Ir. R. Arts is a PhD student at our university. His main focus is the development of a modular &amp; labor-friendly antibody detection protein which should provide a basis to develop antibody detection assays that are suitable for high-throughput screening and application in low-resource areas.
 </div>
 advised us on our bioluminiscence assay.
 </span>
+</div>
 <br />
 <br />
 <br />
-<h4> Safety support </h4>
+<h4> Safety support </h4><img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton14" class="spoilerbutton"><div class="spoiler" id="spoiler14">
-<br /><br />
 <h5> GMO regulations at the TU/e</h5>
 <br />
 <span class="tekst1">
 Dr. M. de Liefde - van Beest <img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton8" class="spoilerbutton"><div class="spoiler" id="spoiler8">
-Dr. M. de Liefde- van Beest is operating in the Department of Biomedical Engineering section Soft Tissue Biomechanics &amp; Tissue engineering at our university. She is an expert in the field of biosafety.
+Dr. M. de Liefde - van Beest is operating in the Department of Biomedical Engineering section Soft Tissue Biomechanics &amp; Tissue engineering at our university. She is an expert in the field of biosafety.
 </div>
   is biosafety officer at our university and informed us about GMO regulations.
@@ Line 127: / Line 142: @@
 gave us a lab tour and granted us lab access.
 </span>
+</div>
 <br />
 <br />
@@ Line 132: / Line 148: @@
-<h4> Policy practice support </h4>
+<h4> Policy Practices support </h4><img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton15" class="spoilerbutton"><div class="spoiler" id="spoiler15">
-<br /><br />
-<h5> Helpful meetings </h5>
-<br />
 <span class="tekst1">
-Claudette de Vries and Robert Geertsma of the National Institute for Public Health and the Environment (RIVM) and Eric van den Akker of the GMO bureau (RIVM).
+We were helped by many people for the Policy Practices part of our project. You can find a complete list of all the people who helped us out <a href="https://2015.igem.org/Team:TU_Eindhoven/Policy_Practices/Stakeholders">here</a>.
 </span>
-<br /><br />
-<h5> Calling with ... </h5>
-<br />
-<span class="tekst1">
-We had an interesting correspondence with Dr. Dhiredj Jagesar
-<img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton10" class="spoilerbutton"><div class="spoiler" id="spoiler10">
-Dr. Dhiredj Jagesar is a scientist at DSM Biotechnology Center. His PhD thesis involved Intercomponent Interactions and Mobility in Hydrogen-Bonded Rotaxanes.<br />
-Currently, he's developing a sensor for real-time detection of antibiotic concentration in (cow) milk.
 </div>
-of DSM. Due to his field of research he drew our attention to some interesting topics concerning biosensors in the commercial world.
-</span>
 <br />
 <br />
 <br />
-<h4> Collaborations </h4>
+<h4> Collaborations </h4><img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton16" class="spoilerbutton"><div class="spoiler" id="spoiler16">
-<br /><br />
 <h5> Amoy newsletter </h5>
 <br />
@@ Line 206: / Line 207: @@
 </div>
 </span>
+</div>
 <br />
 <br />
 <br />
-<h4> Wiki support </h4>
+<h4> Wiki support </h4><img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton17" class="spoilerbutton"><div class="spoiler" id="spoiler17">
-<br /><br />
 <span class="tekst1">
 We built the wiki ourselves. For designing the website we used: <br /><br />
@@ Line 225: / Line 226: @@
 "Team" icon by Björn Andersson <br />
 "Project" icon by Mister Pixel <br />
+"Results" icon by Simple Icons <br />
 "Policy Practices" icon by Rémy Médard <br/>
+"Modeling" icon by Creative Stall <br />
 "Sponsors" icon by Marco Galtarossa <br />
 "Facebook" icon by Niels Gesquiere <br />
@@ Line 232: / Line 235: @@
 "Arrow up" icon by Riley Shaw <br />
 "Arrow down" icon by Riley Shaw <br />
+"Cloning Guide" icon by Joao Paulo <br />
+"Collaboration Attributions" by Marcio Duarte <br />
+"Safety" icon by OCHA visual information unit <br />
+"Report" by João Marcelo Ribeiro, BR <br />
+"Filter" by Anusha Narvekar, IN <br />
+"Arrow up" by P.J. Onori
 </span>
 </div>
@@ Line 242: / Line 251: @@
 The font we use on our wiki is designed by Alessio Laiso and released under the SIL Open Font License, which makes it free to use for both personal and commercial use. More info on the Aleo Font can be found <a href="http://fontfabric.com/aleo-free-font/" target="_blank">here</a>.
 </span>
+</div>
 <br />
 <br />
+<br />
+<h4> Miscellaneous </h4><img src="https://static.igem.org/mediawiki/2015/8/87/TU_Eindhoven_Ingeklapt.png" id="spoilerbutton18" class="spoilerbutton"><div class="spoiler" id="spoiler18">
+<h5> Easter Eggs</h5>
+<br />
+<span class="tekst1">
+Iggy Azalea and Britney Spears for their contribution to the <a href="https://2015.igem.org/Team:TU_Eindhoven/Team/Members" target="_blank">memory</a>. (Yes, it's really there; try clicking on Hans' photo)<br />
+Tess Gaerthé for her contribution to the <a href="https://2015.igem.org/Team:TU_Eindhoven/Team/Members" target="_blank">memory</a>. (Click on Cas, her greatest fan; both literally and figuratively)<br />
+Yeshi de Bruin for her debut album "Yeshi Summer Mix"<br />
+Jaap Valkhoff and the Feijenoord legion for "Hand in hand, kameraden"<br />
+The M-Kids with their hitsong "SMS" <br />
+Tiger Love's "Pussy Cocaine" is a well appreciated addition to our memory, especially for Jan-Willem<br />
+The He-Man rendition of "What's Up", originally sung by 4 Non Blondes <br />
+Cents' "P.I.M.P."; the song of Kwankwan, our local G<br />
+</span>
+</div>
+</div>
 <html/>

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