Difference between revisions of "Team:Lethbridge"

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{{Lethbridge}}
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<html>
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<html>
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    <head>
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<h2> Welcome to iGEM 2015! </h2>
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        <title>iGEM</title>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h4>Before you start: </h4>
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        <meta charset="UTF-8">
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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</ul>
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<div class="highlightBox">
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        <!-- CSS -->
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<h4> Styling your wiki </h4>
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        <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:Lethbridge/main_css?action=raw&ctype=text/css">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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        <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:Lethbridge/index_css?action=raw&ctype=text/css">
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</div>
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<h4> Editing your wiki </h4>
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        <!-- JAVASCRIPT -->
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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        <script type="text/javascript" src="https://2015.igem.org/Team:Lethbridge/jQuery2-1-3_js?action=raw&ctype=text/javascript"></script>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Lethbridge&action=edit"> Click here to edit this page! </a></p>
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        <script type="text/javascript" src="https://2015.igem.org/Team:Lethbridge/index_js?action=raw&ctype=text/javascript"></script>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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    </head>
  
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    <body>
  
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<h4>Templates </h4>
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        <div id="wrapper">
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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            <div id="menu_bar">
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                <ul>
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                    <li><a href="https://2015.igem.org/">iGEM</a></li>
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                    <li><a href="https://2015.igem.org/Team:Lethbridge">RNAiCare</a></li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Project">Project</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Description">Description</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Design">Design</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Results">Results</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Production">Production</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Judging">Judging</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Parts">Parts</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Basic_Part">Basic Parts</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Composite_Part">Composite Parts</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Measurement">Measurement</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Practices">Practices</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Risks">Risks</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Stakeholders">Stakeholders</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Current">Current Problems</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Notebook">Notebook</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_July">July</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_August">August</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_September">September</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Team">Team</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Team_Members">Members</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Attributions">Attributions</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li>
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                        </ul>
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                    </li>
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                </ul>
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            </div>
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            <div id="content_wrapper">
  
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<h4>Tips</h4>
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                <div id="main_banner_container">
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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                    <div class="banner_title">Pesticides for the Future</div>
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<ul>
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                    <div class="banner_buttons">
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Design">Design</a>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Results">Results</a>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Practices">Practice</a>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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                    </div>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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                </div>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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                <div class="info_box" id="info_1" style="background:#f1f6f3">
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                    <h2 style="color:#333;font-size:48px;">World Food Shortage</h2>
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                    <p style="font-size:36px;">By 2030 the Earth will have about 8.4 billion people living on it.</p>
 +
                    <p style="font-size:36px;">The UN estimates that we will need to increase agricultural production by 70% to feed the inhabitants of the world by 2050.</p>
 +
                    <p style="font-size:36px;">We need a better way to ensure the safety and productivity of our food supply across the globe.</p>
 +
                    <img src="https://static.igem.org/mediawiki/2015/6/6f/Uleth15_Globe.png" alt="earth" style="display:block;margin:0 auto;width:25%;" />
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                </div>
  
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<h4>Inspiration</h4>
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                <div class="info_box" id="info_2">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                  <div style="width:50%;height:100%;">
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<ul>
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                    <h2 style="color:#fff;font-size:48px;">Current Pesticides</h2>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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                    <p style="font-size:36px;">In the past we have relied on small molecule pesticides to control species that threaten our food supply. However, with the development of resistant pest species, harmful off-target effects, persistence in the environment, and diminishing returns, conventional pesticides have shown themselves to be unsustainable for a growing planet.</p>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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                  </div>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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                </div>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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                <div class="info_box" id="info_3">
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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                  <div style="width:50%;height:100%;margin-left:50%;">
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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                    <h2 style="color:#fff;font-size:48px;">Our Solution</h2>
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                    <p style="font-size:36px;color:#fff;">We propose an alternative: a biosynthetic pesticide that is:</p>
 +
                    <ul style="font-size:36px;color:#fff;list-style:none;padding-left:32px;">
 +
                        <li>Species specific</li>
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                        <li>Cheap to produce</li>
 +
                        <li>Easy to apply</li>
 +
                        <li>Safe for the environment.</li>
 +
                    </ul>
 +
                  </div>
 +
                </div>
  
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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                <div class="info_box" id="info_4">
 +
                    <a href="https://2015.igem.org/Team:Lethbridge/Design"><h2 style="color:#333;font-size:48px;">Design</h2></a>
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                    <p style="font-size:36px;color:#333;">RNA Interference mediated by double stranded RNA (dsRNA) has been a powerful tool for research since it’s discovery. RNA is: transient, non-heritable, auto-hydrolyzing, making it the perfect molecule for pesticide use. Our goal is to produce highly pure and specific siRNAs to induce silencing in pest species using E.coli. In addition to increasing the scope of potential target species that siRNAs can combat.</p>
 +
                    <img src="https://static.igem.org/mediawiki/2015/7/78/Uleth15_RiboYolo.png" alt="Ribosyme" style="display:block;margin:0 auto;width:25%;" />
 +
                </div>
  
 +
                <div class="info_box" id="info_5">
 +
                    <a href="https://2015.igem.org/Team:Lethbridge/Results"><h2 style="color:#fff;font-size:48px;">Results</h2></a>
 +
                    <p style="font-size:36px;color:#fff;">We successfully produced a theophylline aptazyme in vitro which had previously only been characterized in vivo.</p>
 +
                    <p style="font-size:36px;color:#fff;">In addition, we successfully purified RNA using a Ribozyme Affinity Purification strategy.</p>
 +
                    <p style="font-size:36px;color:#fff;">We were able to knockdown genes in Fusarium graminearum using directly-applied dsRNA which had previously never been demonstrated.</p>
 +
                </div>
  
 +
                <div class="info_box" id="info_7">
 +
                    <a href="https://2015.igem.org/Team:Lethbridge/Practices"><h2 style="color:#fff;font-size:48px;">Practices</h2></a>
 +
                    <p style="font-size:36px;color:#fff;">We took an in depth look at the ethical issues surrounding our project, met with researchers, farmers, and pesticide producers.</p>
 +
                    <img src="https://static.igem.org/mediawiki/2015/3/39/Uleth15_WheatHands.jpg" alt="Wheat hands" style="margin:0 auto;width:60%;" />
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                </div>
  
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</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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                <div class="info_box" id="info_8" style="background:#f1f6f3;">
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                    <a href="https://2015.igem.org/Team:Lethbridge/Team_Members"><h2 style="color:#333;font-size:48px;text-align:center;">Meet the team behind the project:</h2></a>
 +
                    <a href="https://2015.igem.org/Team:Lethbridge/Team_Members" style="display:block;width:100%;"><img src="https://static.igem.org/mediawiki/2015/8/8e/Uleth15_TeamPhoto.jpg" height="50%" width="50%" style="display:block;margin:0 auto;" ></a>
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                </div>
  
 +
                <div class="info_box" id="index_sponsors">
 +
                        <a href="https://www.idtdna.com/site" id="index_sponsor_IDT"></a>
 +
                        <a href="http://www.geneious.com/" id="index_sponsor_geneious"></a>
 +
                        <a href="http://www.albertatechfutures.ca/" id="index_sponsor_AITF"></a>
 +
                        <a href="http://pearlbiotech.com/" id="index_sponsor_pearl"></a>
 +
                        <a href="http://www.agr.gc.ca/eng/home/" id="index_sponsor_AAFC"></a>
 +
                        <a href="http://www.uleth.ca/research/alberta-rna-research-and-training-institute-arrti" id="index_sponsor_AARTI"></a>
 +
                        <a href="http://www.uleth.ca/" id="index_sponsor_Uleth"></a>
 +
                </div>
 +
 +
            </div>
 +
   
 +
        </div>
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 +
    </body>
 
</html>
 
</html>

Latest revision as of 03:56, 19 September 2015

iGEM

World Food Shortage

By 2030 the Earth will have about 8.4 billion people living on it.

The UN estimates that we will need to increase agricultural production by 70% to feed the inhabitants of the world by 2050.

We need a better way to ensure the safety and productivity of our food supply across the globe.

earth

Current Pesticides

In the past we have relied on small molecule pesticides to control species that threaten our food supply. However, with the development of resistant pest species, harmful off-target effects, persistence in the environment, and diminishing returns, conventional pesticides have shown themselves to be unsustainable for a growing planet.

Our Solution

We propose an alternative: a biosynthetic pesticide that is:

  • Species specific
  • Cheap to produce
  • Easy to apply
  • Safe for the environment.

Design

RNA Interference mediated by double stranded RNA (dsRNA) has been a powerful tool for research since it’s discovery. RNA is: transient, non-heritable, auto-hydrolyzing, making it the perfect molecule for pesticide use. Our goal is to produce highly pure and specific siRNAs to induce silencing in pest species using E.coli. In addition to increasing the scope of potential target species that siRNAs can combat.

Ribosyme

Results

We successfully produced a theophylline aptazyme in vitro which had previously only been characterized in vivo.

In addition, we successfully purified RNA using a Ribozyme Affinity Purification strategy.

We were able to knockdown genes in Fusarium graminearum using directly-applied dsRNA which had previously never been demonstrated.

Practices

We took an in depth look at the ethical issues surrounding our project, met with researchers, farmers, and pesticide producers.

Wheat hands