Difference between revisions of "Team:Michigan/Notebook/September"

 
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<img src="http://www.facebook.com/l.php?u=http%3A%2F%2F2015.igem.org%2FFile%3ASwitchThrombin.png&h=IAQEgSM9w" alt="MostImportant" style="width:304px;height:228px;">
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09/07/2015
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we wanted to replicate results from previous experiment and try to detect lower thrombin concentrations to prove the sensitivity of our system
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1 = Negative control (switch 1.0 + trigger-aptamer No Thrombin)<br>
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2 = Positive control (switch 1.0 + trigger)<br>
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3= Switch 1.0 + trigger + aptamer + 25uM Thrombin<br>
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4= Switch 1.0 + trigger + aptamer + 5uM Thrombin<br>
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5= Switch 1.0 + trigger + aptamer + 2.5uM Thrombin<br>
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6= Switch 1.0 + trigger + aptamer + 1.0uM Thrombin<br>
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7= Switch 1.0 + trigger + aptamer + 0.5uM Thrombin<br>
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8= Switch 1.0 + trigger + aptamer + 0.1uM Thrombin<br>
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<img src="https://static.igem.org/mediawiki/2015/3/36/SwitchThrombin.png" alt="MostImportant" style="width:504px;height:428px;">
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<img src="https://static.igem.org/mediawiki/2015/1/19/Gliadin_titration.png" alt="Gliadin" style="width:504px;height:428px;">
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<img src="https://static.igem.org/mediawiki/2015/a/a7/Table_thing.png" alt="TableThing" style="width:504px;height:428px;">
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Latest revision as of 03:58, 19 September 2015

September

Work plan:

LacZ into Thrombin plasmid:

  • cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
  • transform in vitro LacZ part again
  • plasmid is ready, digest with HindiIII and A
  • gel extract plasmid, measure dna concentration

LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence

Things to order:
  • aptamer and trigger for Gliadin
  • switch for Gliadin with GFP (check design folder)
  • PfLDH protein
  • aptamer and trigger for Pfldh
  • aptamer and trigger for Kanamycin
09/07/2015

we wanted to replicate results from previous experiment and try to detect lower thrombin concentrations to prove the sensitivity of our system

1 = Negative control (switch 1.0 + trigger-aptamer No Thrombin)
2 = Positive control (switch 1.0 + trigger)
3= Switch 1.0 + trigger + aptamer + 25uM Thrombin
4= Switch 1.0 + trigger + aptamer + 5uM Thrombin
5= Switch 1.0 + trigger + aptamer + 2.5uM Thrombin
6= Switch 1.0 + trigger + aptamer + 1.0uM Thrombin
7= Switch 1.0 + trigger + aptamer + 0.5uM Thrombin
8= Switch 1.0 + trigger + aptamer + 0.1uM Thrombin



MostImportant

Gliadin

TableThing