Difference between revisions of "Team:Michigan/Notebook/September"
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− | <img src="https://static.igem.org/mediawiki/2015/3/36/SwitchThrombin.png" alt="MostImportant" style="width: | + | 09/07/2015 |
+ | <br><br> | ||
+ | we wanted to replicate results from previous experiment and try to detect lower thrombin concentrations to prove the sensitivity of our system | ||
+ | <br><br> | ||
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+ | 1 = Negative control (switch 1.0 + trigger-aptamer No Thrombin)<br> | ||
+ | 2 = Positive control (switch 1.0 + trigger)<br> | ||
+ | 3= Switch 1.0 + trigger + aptamer + 25uM Thrombin<br> | ||
+ | 4= Switch 1.0 + trigger + aptamer + 5uM Thrombin<br> | ||
+ | 5= Switch 1.0 + trigger + aptamer + 2.5uM Thrombin<br> | ||
+ | 6= Switch 1.0 + trigger + aptamer + 1.0uM Thrombin<br> | ||
+ | 7= Switch 1.0 + trigger + aptamer + 0.5uM Thrombin<br> | ||
+ | 8= Switch 1.0 + trigger + aptamer + 0.1uM Thrombin<br> | ||
+ | <br><br><br> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2015/3/36/SwitchThrombin.png" alt="MostImportant" style="width:504px;height:428px;"> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/1/19/Gliadin_titration.png" alt="Gliadin" style="width:504px;height:428px;"> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/a/a7/Table_thing.png" alt="TableThing" style="width:504px;height:428px;"> | ||
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Latest revision as of 03:58, 19 September 2015
September
Work plan:
LacZ into Thrombin plasmid:
- cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
- transform in vitro LacZ part again
- plasmid is ready, digest with HindiIII and A
- gel extract plasmid, measure dna concentration
LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence
Things to order:
- aptamer and trigger for Gliadin
- switch for Gliadin with GFP (check design folder)
- PfLDH protein
- aptamer and trigger for Pfldh
- aptamer and trigger for Kanamycin
we wanted to replicate results from previous experiment and try to detect lower thrombin concentrations to prove the sensitivity of our system
1 = Negative control (switch 1.0 + trigger-aptamer No Thrombin)
2 = Positive control (switch 1.0 + trigger)
3= Switch 1.0 + trigger + aptamer + 25uM Thrombin
4= Switch 1.0 + trigger + aptamer + 5uM Thrombin
5= Switch 1.0 + trigger + aptamer + 2.5uM Thrombin
6= Switch 1.0 + trigger + aptamer + 1.0uM Thrombin
7= Switch 1.0 + trigger + aptamer + 0.5uM Thrombin
8= Switch 1.0 + trigger + aptamer + 0.1uM Thrombin