Difference between revisions of "Team:HokkaidoU Japan/Composite Part"

 
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<h1> Composite Part</h1>
 
<h1> Composite Part</h1>
  
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<br><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1714000">BBa_K1714000</a></br>
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<br><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1714007">BBa_K1714007</a></br>
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<p>Our favorite composite part is BBa_K1714000. This part has pBad/araC as promoter, RBS B0034 as RBS, Ag43 thanatin as α-domain as CDS, double terminator as termnator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. Comparing the toxicity for host cells and thanatins production.
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<p>Our favorite composite part is <a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1714007">BBa_K1714007</a>.</br>This part has P<sub>BAD</sub> as a promoter, <a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</a> as an RBS, thanatin inserted Ag43 as a CDS, double terminator as a terminator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. This part successfully secret thanatins and caused toxicity. So we succeeded in making new way to secret AMPs by using Ag43.
  
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<h4>Note</h4>
 
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
 
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<a href="https://2015.igem.org/Team:HokkaidoU_Japan/Parts">←Parts</a></p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
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Latest revision as of 03:47, 7 October 2015

main3

Parts

Composite Part


BBa_K1714007

Our favorite composite part is BBa_K1714007.
This part has PBAD as a promoter, BBa_B0034 as an RBS, thanatin inserted Ag43 as a CDS, double terminator as a terminator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. This part successfully secret thanatins and caused toxicity. So we succeeded in making new way to secret AMPs by using Ag43.

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