Difference between revisions of "Team:SVA-NYC/Chemical-Parts"

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<h2>Chemical Parts</h2>
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<h1>Chemical Parts</h1>
  
 
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  <img src="https://static.igem.org/mediawiki/2015/8/80/Test_svg.svg" alt="test SVG file"  width="90%" height="90%" />
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  <img class="featurette-image img-responsive" alt="Color Kit"  src="https://static.igem.org/mediawiki/2015/a/a6/Color-kit-test.jpg">
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</div>
  
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<h3>LaMotte Kit Model STH Series - Combination Soil Testing Outfits (product ID #5029) Reagents:</h3>
 +
<p>
 +
Soil Flocculating Reagent (5643WT) <br>
 +
Universal Extracting Solution (5173) <br>
 +
Duplex Indicator (2221) <br>
 +
Bromcresol Green (2207)<br>
 +
Chlorphenol Red (2209)<br>
 +
Bromthymol Blue (2210)<br>
 +
Phenol Red (2211)<br>
 +
Thymol Blue (2213)<br>
 +
Potassium Reagent B Tablets (5161A)<br>
 +
Potassium Reagent C (5162) <br>
 +
Phosphorus Reagent 2 (5156) <br>
 +
Phosphorus Test Tablets (5706A) <br>
 +
Nitrate Reagent #1 (5146) <br>
 +
Nitrate Reagent #2 Powder (5147)<br>
 +
Humus Screening Reagent Powder (5119) <br>
 +
Calcium Test Solution (5108PS) <br>
 +
Ammonia Nitrogen Test Solution (5103PS) <br>
 +
Magnesium Test Solution 1 (5140)<br>
 +
Magnesium and Manganese Test Solution #2 (5145WT) <br>
 +
Manganese Periodate Reagent (6311)<br>
 +
Aluminum Test Solution (5101) <br>
 +
Nitrite Nitrogen Reagent 1 (5151WT) <br>
 +
Nitrite Nitrogen Reagent 2 (5152WT) <br>
 +
Nitrite Nitrogen Reagent 3 (5153WT) <br>
 +
Ferric Iron Test Solution (5116PS) <br>
 +
Sulfate Test Solution (5171) <br>
 +
Chloride Test Solution (5111) <br>
  
<div class="row">
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<hr>
  <div class="col-md-6">
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    <p>A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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  </p>
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  </div>
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  <div class="col-md-6">
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  <p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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  </div>
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</div>
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</div>
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<h3>Standards:</h3>
  
 +
<p>
 +
Chloride standard from NaCl<br>
 +
Total M.W.= 58.44<br>
 +
Cl M.W.= 35.5<br>
 +
500ppm = .014084507 mol =<br>
 +
0.20577469 grams in 250 mL<br>
  
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<hr>
  
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Potassium standard from KCl<br>
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Total M.W.= 74.55<br>
 +
K M.W.= 39.0983<br>
 +
400ppm = .0028139 mol =<br>
 +
0.052 grams in 250 mL<br>
 +
 
 +
<hr>
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 +
 
 +
Sulfate standard from MgSO4 * 7H2O<br>
 +
Total M.W.= 246.47<br>
 +
SO4 M.W.= 96.06<br>
 +
2000 ppm = .02082032 mol =<br>
 +
1.28289611 grams in 250 mL<br>
 +
 
 +
<hr>
 +
 
 +
 
 +
Nitrite standard from NaNO2<br>
 +
Total M.W.= NaNO2 68.9953<br>
 +
NO2 M.W.= 46.01<br>
 +
50 ppm = .00108672 mol =<br>
 +
.01874465 grams in 250 mL<br>
 +
 
 +
<hr>
 +
 
 +
 
 +
Nitrate standard from KNO3<br>
 +
Total M.W.= 101.1<br>
 +
NO3 M.W.= 62.0049<br>
 +
75 ppm = .00120958 mol =<br>
 +
.03057218 grams in 250 mL<br>
 +
 
 +
<hr>
 +
 
 +
 
 +
Calcium standard from CaCl2<br>
 +
Total M.W.= 110.98<br>
 +
Ca M.W.= 40.98<br>
 +
2800 ppm = .06832601 mol<br>
 +
1.89570522 grams in 250 mL<br>
 +
 
 +
<hr>
 +
 
 +
 
 +
Aluminum standard from AlK(SO4)2 * 12H20<br>
 +
Total M.W.= 474.39<br>
 +
Al M.W.= 26.981538<br>
 +
125 ppm = .0046328 mol =<br>
 +
.54943819 grams in 250 mL<br>
 +
 
 +
<hr>
 +
 
 +
Manganese standard from MnSO4*H2O<br>
 +
Total M.W.= 169.01<br>
 +
Mn M.W.= 54.938044<br>
 +
molar ratio is 0.32505795<br>
 +
40 ppm = 0.00023667(total) = 0.00007693 (Mn)<br>
 +
0.00325055g in 250mL<br>
 +
0.650g in 50mL = 1000x<br>
 +
250uL of 1000x in 250mL = 1x 40ppm Mn Standard<br>
 +
 
 +
<hr>
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 +
Phosphate standard from KH2PO4<br>
 +
Total M.W.= 136.09<br>
 +
P M.W.= 30.973762<br>
 +
200 ppm = .00645708 =<br>
 +
.21968594 grams in 250 mL<br>
 +
 
 +
<hr>
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 +
 
 +
Ammonia standard from NH4Cl<br>
 +
Total M.W.= 53.49<br>
 +
NH3 M.W.= 17.031<br>
 +
150 ppm = .00880747 mol =<br>
 +
.11777788 grams in 250 mL<br>
 +
 
 +
 
 +
<hr>
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 +
Magnesium Standard from MgCl2 * 6H2O<br>
 +
Total M.W.= 203.3<br>
 +
Mg M.W.= 24.305<br>
 +
150 ppm = .00617157 mol =<br>
 +
.31367003 grams in 250 mL<br>
 +
 
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<hr>
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 +
Ferric Iron standard from Fe2(SO4)3 * 9H2O<br>
 +
Total M.W.= 562<br>
 +
Fe M.W.= 55.845<br>
 +
62.5 ppm = .00055958 mol =<br>
 +
.07862163 grams in 250 mL<br>
 +
 
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Latest revision as of 23:10, 7 October 2015


soiled

SVA-NYC

Chemical Parts

Color Kit

LaMotte Kit Model STH Series - Combination Soil Testing Outfits (product ID #5029) Reagents:

Soil Flocculating Reagent (5643WT)
Universal Extracting Solution (5173)
Duplex Indicator (2221)
Bromcresol Green (2207)
Chlorphenol Red (2209)
Bromthymol Blue (2210)
Phenol Red (2211)
Thymol Blue (2213)
Potassium Reagent B Tablets (5161A)
Potassium Reagent C (5162)
Phosphorus Reagent 2 (5156)
Phosphorus Test Tablets (5706A)
Nitrate Reagent #1 (5146)
Nitrate Reagent #2 Powder (5147)
Humus Screening Reagent Powder (5119)
Calcium Test Solution (5108PS)
Ammonia Nitrogen Test Solution (5103PS)
Magnesium Test Solution 1 (5140)
Magnesium and Manganese Test Solution #2 (5145WT)
Manganese Periodate Reagent (6311)
Aluminum Test Solution (5101)
Nitrite Nitrogen Reagent 1 (5151WT)
Nitrite Nitrogen Reagent 2 (5152WT)
Nitrite Nitrogen Reagent 3 (5153WT)
Ferric Iron Test Solution (5116PS)
Sulfate Test Solution (5171)
Chloride Test Solution (5111)


Standards:

Chloride standard from NaCl
Total M.W.= 58.44
Cl M.W.= 35.5
500ppm = .014084507 mol =
0.20577469 grams in 250 mL


Potassium standard from KCl
Total M.W.= 74.55
K M.W.= 39.0983
400ppm = .0028139 mol =
0.052 grams in 250 mL

Sulfate standard from MgSO4 * 7H2O
Total M.W.= 246.47
SO4 M.W.= 96.06
2000 ppm = .02082032 mol =
1.28289611 grams in 250 mL

Nitrite standard from NaNO2
Total M.W.= NaNO2 68.9953
NO2 M.W.= 46.01
50 ppm = .00108672 mol =
.01874465 grams in 250 mL

Nitrate standard from KNO3
Total M.W.= 101.1
NO3 M.W.= 62.0049
75 ppm = .00120958 mol =
.03057218 grams in 250 mL

Calcium standard from CaCl2
Total M.W.= 110.98
Ca M.W.= 40.98
2800 ppm = .06832601 mol
1.89570522 grams in 250 mL

Aluminum standard from AlK(SO4)2 * 12H20
Total M.W.= 474.39
Al M.W.= 26.981538
125 ppm = .0046328 mol =
.54943819 grams in 250 mL

Manganese standard from MnSO4*H2O
Total M.W.= 169.01
Mn M.W.= 54.938044
molar ratio is 0.32505795
40 ppm = 0.00023667(total) = 0.00007693 (Mn)
0.00325055g in 250mL
0.650g in 50mL = 1000x
250uL of 1000x in 250mL = 1x 40ppm Mn Standard

Phosphate standard from KH2PO4
Total M.W.= 136.09
P M.W.= 30.973762
200 ppm = .00645708 =
.21968594 grams in 250 mL

Ammonia standard from NH4Cl
Total M.W.= 53.49
NH3 M.W.= 17.031
150 ppm = .00880747 mol =
.11777788 grams in 250 mL

Magnesium Standard from MgCl2 * 6H2O
Total M.W.= 203.3
Mg M.W.= 24.305
150 ppm = .00617157 mol =
.31367003 grams in 250 mL

Ferric Iron standard from Fe2(SO4)3 * 9H2O
Total M.W.= 562
Fe M.W.= 55.845
62.5 ppm = .00055958 mol =
.07862163 grams in 250 mL

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