Difference between revisions of "Team:UChicago"

 
(15 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{UChicago/Header}}
 
{{UChicago/Header}}
−
<html>
+
<html>
−
<h2> Welcome to iGEM 2015! </h2>
+
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
  
−
<h4>Before you start: </h4>
+
<head>
−
<p> Please read the following pages:</p>
+
<meta http-equiv=Content-Type content="text/html; charset=windows-1252">
−
<ul>
+
<meta name=Generator content="Microsoft Word 15 (filtered)">
−
<li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
+
<style>
−
<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
+
<!--
−
</ul>
+
  /* Font Definitions */
 +
@font-face
 +
{font-family:"Cambria Math";
 +
panose-1:2 4 5 3 5 4 6 3 2 4;}
 +
@font-face
 +
{font-family:Calibri;
 +
panose-1:2 15 5 2 2 2 4 3 2 4;}
 +
@font-face
 +
{font-family:Georgia;
 +
panose-1:2 4 5 2 5 4 5 2 3 3;}
 +
@font-face
 +
{font-family:Garamond;
 +
panose-1:2 2 4 4 3 3 1 1 8 3;}
 +
/* Style Definitions */
 +
p.MsoNormal, li.MsoNormal, div.MsoNormal
 +
{margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:8.0pt;
 +
margin-left:0in;
 +
line-height:107%;
 +
font-size:12.0pt;
 +
font-family:"Times New Roman",serif;}
 +
h1
 +
{mso-style-link:"Heading 1 Char";
 +
margin-top:12.0pt;
 +
margin-right:0in;
 +
margin-bottom:0in;
 +
margin-left:0in;
 +
margin-bottom:.0001pt;
 +
line-height:107%;
 +
page-break-after:avoid;
 +
font-size:16.0pt;
 +
font-family:"Calibri Light",sans-serif;
 +
color:#2E74B5;
 +
font-weight:normal;}
 +
a:link, span.MsoHyperlink
 +
{color:#0563C1;
 +
text-decoration:underline;}
 +
a:visited, span.MsoHyperlinkFollowed
 +
{color:#954F72;
 +
text-decoration:underline;}
 +
p
 +
{margin-right:0in;
 +
margin-left:0in;
 +
font-size:12.0pt;
 +
font-family:"Times New Roman",serif;}
 +
span.Heading1Char
 +
{mso-style-name:"Heading 1 Char";
 +
mso-style-link:"Heading 1";
 +
font-family:"Calibri Light",sans-serif;
 +
color:#2E74B5;}
 +
.MsoChpDefault
 +
{font-size:12.0pt;}
 +
.MsoPapDefault
 +
{margin-bottom:8.0pt;
 +
line-height:107%;}
 +
@page WordSection1
 +
{size:8.5in 11.0in;
 +
margin:1.0in 1.0in 1.0in 1.0in;}
 +
div.WordSection1
 +
{page:WordSection1;}
 +
-->
 +
</style>
  
−
<div class="highlightBox">
+
</head>
−
<h4> Styling your wiki </h4>
+
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
+
−
</div>
+
  
−
<h4> Editing your wiki </h4>
+
<body lang=EN-US link="#0563C1" vlink="#954F72">
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
−
<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:UChicago&action=edit"> Click here to edit this page! </a></p>
+
−
<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
+
  
 +
<div class=WordSection1>
  
−
<h4>Templates </h4>
+
<h1 align=center style='text-align:center'><b><span style='font-size:32.0pt;
−
<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
+
line-height:107%;font-family:"Calibri",sans-serif;color:#C00000'>Transplanting
−
<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
+
KaiABC: Reduce your jet lag with this one weird trick!</span></b></h1>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><span style='font-size:16.0pt;font-family:"Georgia",serif'>&nbsp;</span></p>
  
−
<h4>Tips</h4>
+
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
text-align:center;line-height:normal'><b><span style='font-size:16.0pt;
−
<ul>
+
font-family:"Georgia",serif;color:black'><img width=445 height=261
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
id="Picture 6" src="/wiki/images/1/19/UChicago2015Home_filesimage001.jpg"><img width=412 height=259
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
id="Picture 7" src="/wiki/images/1/15/UChicago2015Home_filesimage002.jpg"><img width=433 height=259
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
id="Picture 8" src="/wiki/images/b/b3/UChicago2015Home_filesimage003.gif"></span></b></p>
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>  
+
  
 +
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
 +
text-align:center;line-height:normal'><i><span style='font-family:"Arial",sans-serif;
 +
color:black'>Do you see these images in your jet-lag-drunken nightmares? I know
 +
I do.</span></i></p>
  
−
<h4>Inspiration</h4>
+
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
−
<ul>
+
color:black'>                                                                                                            </span></b></p>
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
  
−
<h4> Uploading pictures and files </h4>
+
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
−
<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
−
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
color:black'>Have you ever felt tired as a weary traveler after a whole day of
 +
flying in an unfamiliar city? So doggone tired that you would do anything just
 +
to feel refreshed anything? Then just click </span></b><a
 +
href="https://2015.igem.org/Team:UChicago/Description"><b><span
 +
style='font-size:16.0pt;font-family:"Georgia",serif'>here</span></b></a><b><span
 +
style='font-size:16.0pt;font-family:"Georgia",serif;color:black'> to buy the
 +
ultimate cure to jet lag! Or just give us your credit card information </span></b><a
 +
href="https://2015.igem.org/Team:UChicago/Description"><b><span
 +
style='font-size:16.0pt;font-family:"Georgia",serif'>here</span></b></a><b><span
 +
style='font-size:16.0pt;font-family:"Georgia",serif;color:black'> and we’ll
 +
ship it to you pronto!</span></b></p>
  
−
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
+
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 +
color:black'>&nbsp;</span></b></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 +
color:black'>…Just kidding! We’re not trying to scam you here, we’re the
 +
UChicago 2015 iGEM team and we’re trying to create bacteria that can <u>reduce
 +
or cure your jet lag</u> (as well as sleeping disorders).</span></b></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 +
color:black'>&nbsp;</span></b></p>
  
−
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><span style='font-size:16.0pt;font-family:"Garamond",serif;color:black'>We
 +
actually have science to back us up! Currently, there exist no working 24-hour
 +
oscillator BioBricks. We are addressing this problem by transplanting the
 +
circadian rhythm-generating KaiABC system from cyanobacteria into E. coli and
 +
optimizing its function by controlling protein stoichiometry and adding
 +
accessory proteins. Such a BioBrick would be important for any application in
 +
which gene expression needs to be regulated on a 24-hour schedule. For example,
 +
it is often important to release a drug at a specific time of the day. As a
 +
proof-of-concept application, we implemented this clock as a potential solution
 +
to jet lag and sleep disorders by engineering a strain of E. coli that
 +
facilitates the production of a melatonin precursor in the gut on a 24-hour
 +
cycle. Thus, our project hopes to demonstrate the application of a robust
 +
KaiABC-based clock in timed drug dosage, as well as introduces an oscillator
 +
BioBrick into the synthetic biology community. </span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><span style='font-size:16.0pt;font-family:"Georgia",serif;color:black'>&nbsp;</span></p>
 +
 
 +
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
 +
text-align:center;line-height:normal'><img border=0 width=662 height=832
 +
id="Picture 4" src="/wiki/images/c/ca/UChicago2015Home_filesimage004.png"></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal'><i><span style='font-family:"Arial",sans-serif;color:black'>Figure 1:
 +
A) The first leg of our project focuses on reconstituting the KaiABC oscillator
 +
system. B) The second leg of our journey (see what we did there?) focuses on
 +
creating a readout system that can process KaiC phosphorylation state into
 +
actual promoter state. Not depicted is the third part of our project, which
 +
aims to secrete AANAT, an enzyme that can produce a melatonin precursor.</span></i></p>
 +
 
 +
<p class=MsoNormal><span style='font-size:16.0pt;line-height:107%;font-family:
 +
"Georgia",serif'>&nbsp;</span></p>
 +
 
 +
<p class=MsoNormal><b><span style='font-size:16.0pt;line-height:107%;
 +
font-family:"Georgia",serif'>Still not convinced? Then click </span></b><a
 +
href="https://2015.igem.org/Team:UChicago/Description"><b><span
 +
style='font-size:16.0pt;line-height:107%;font-family:"Georgia",serif'>here</span></b></a><b><span
 +
style='font-size:16.0pt;line-height:107%;font-family:"Georgia",serif'> to learn
 +
more!</span></b></p>
 +
 
 +
</div>
 +
 
 +
</body>
  
 
</html>
 
</html>
 +
 +
 +
 +
 +
{{UChicago/Footer}}

Latest revision as of 03:51, 9 October 2015

Team UChicago banner.jpg

Transplanting KaiABC: Reduce your jet lag with this one weird trick!

 

Do you see these images in your jet-lag-drunken nightmares? I know I do.

                                                                                                           

Have you ever felt tired as a weary traveler after a whole day of flying in an unfamiliar city? So doggone tired that you would do anything just to feel refreshed anything? Then just click here to buy the ultimate cure to jet lag! Or just give us your credit card information here and we’ll ship it to you pronto!

 

…Just kidding! We’re not trying to scam you here, we’re the UChicago 2015 iGEM team and we’re trying to create bacteria that can reduce or cure your jet lag (as well as sleeping disorders).

 

We actually have science to back us up! Currently, there exist no working 24-hour oscillator BioBricks. We are addressing this problem by transplanting the circadian rhythm-generating KaiABC system from cyanobacteria into E. coli and optimizing its function by controlling protein stoichiometry and adding accessory proteins. Such a BioBrick would be important for any application in which gene expression needs to be regulated on a 24-hour schedule. For example, it is often important to release a drug at a specific time of the day. As a proof-of-concept application, we implemented this clock as a potential solution to jet lag and sleep disorders by engineering a strain of E. coli that facilitates the production of a melatonin precursor in the gut on a 24-hour cycle. Thus, our project hopes to demonstrate the application of a robust KaiABC-based clock in timed drug dosage, as well as introduces an oscillator BioBrick into the synthetic biology community.

 

Figure 1: A) The first leg of our project focuses on reconstituting the KaiABC oscillator system. B) The second leg of our journey (see what we did there?) focuses on creating a readout system that can process KaiC phosphorylation state into actual promoter state. Not depicted is the third part of our project, which aims to secrete AANAT, an enzyme that can produce a melatonin precursor.

 

Still not convinced? Then click here to learn more!



Your Website Title

Like our team Facebook page, Genehackers@UChicago!

Questions? Comments? Send us an email!