Difference between revisions of "Team:Hong Kong-CUHK/Collaborations"

 
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<h2> Collaborations</h2>
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<h1> Collaborations</h1>
 
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<p><font face="Times New Roman" size="4pt">Apart from working on our project, our team also respect the “iGEM values” very much. We believe that through co-operating with other iGEM teams, we can share our passion and assist each other to create better results. </p></font>
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<p>Apart from working on our project, our team also respect the “iGEM values” very much. We believe that through co-operating with other iGEM teams, we can share our passion and assist each other to create better results. </p>
 
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<p><font face="Times New Roman" size="6pt">1. Lab work and modelling collaboration with City University of Hong Kong</p></font>
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<h2>(1) Lab Work and Modelling Collaborations with City University of Hong Kong</h2>
 
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<p><font face="Times New Roman" size="4pt">For this collaboration, our team members visited each other’s university and presented our projects with the team members and professors. During the visit, we have reached an agreement on collaboration of which we would help them to do characterisation on the expression of the lacZY gene. And they helped us to do a part of the modeling for the application part of our project. </p></font>
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<p>For this collaboration, our team members visited each other’s university and presented our projects with the team members and professors. During the visit, we have reached an agreement on collaboration of which we would help them to do characterization on the expression of the <i>lacZY</i> gene. And they helped us to do a part of the modeling for the application part of our project.<br>
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<p><font face="Times New Roman" size="4pt">City provided 2 plasmids for the characterisation:<p></font>
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<p>CityU team provided us 2 plasmids for the characterization:<p>
 
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<p><font face="Times New Roman" size="4pt">1. Pro LacZY</p></font>
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<p>1. Pro LacZY</p>
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<p><font face="Times New Roman" size="4pt">2. Pro LacZ(control)</p></font>
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<p>2. Pro LacZ(control)</p>
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<p><font face="Times New Roman" size="4pt">Theoretically, only the E.coli containing LacZY would absorb ortho-Nitrophenyl-β-galactoside (OPNG) which is a lactose analogue. Then the beta-galactosidase that is produced by the LacZY E.coli would convert the OPNG into galactose and ortho-nitrophenol. The ortho-nitrophenol which is yellow in colour. The ortho-nitrophenol has absorbance at 420nm. Therefore we can measure the OD420 to measure the expression of LacZY. </p></font>
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<p>Theoretically, only the <i>E. coli</i> containing LacZY would absorb ortho-Nitrophenyl-β-galactoside (OPNG) which is a lactose analogue. Then the beta-galactosidase produced by the LacZY <i>E. coli</i> would convert the OPNG into galactose and ortho-nitrophenol, which is yellow in colour and absorbs at 420 nm. Therefore we can measure OD<sub>420</sub> to monitor the expression of LacZY. </p>
 
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<p><font face="Times New Roman" size="4pt">Procedures:</p></font>
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<p>Procedures:</p>
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<p><font face="Times New Roman" size="4pt">1. Prepare an overnight culture of LacZ and LacZY in E.coli </p></font>
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<p>1. Prepare an overnight culture of LacZ and LacZY in <i>E. coli</i>. </p>
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<p><font face="Times New Roman" size="4pt">2. Measure the OD420 of the overnight culture and dilute it into ~0.5OD </p></font>
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<p>2. Measure the OD<sub>420</sub> of the overnight culture and dilute it into ~0.5 OD. </p>
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<p><font face="Times New Roman" size="4pt">3. Add OPNG solution into the sample </p></font>
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<p>3. Add OPNG solution into the sample. </p>
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<p><font face="Times New Roman" size="4pt">4. Measure the OD420 value at 20 minutes intervals </p></font>
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<p>4. Measure OD<sub>420</sub> at 20 min intervals.</p>
 
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<p><font face="Times New Roman" size="6pt">2. International questionnaire with 10 different universities worldwide </p></font>
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<h2>(2) International Questionnaire with 10 Different Universities Worldwide </h2>
 
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<p><font face="Times New Roman" size="4pt">CUHK was honoured to be in cooperation with 10 various different universities to conduct an international questionnaire on synthetic biology and on our respective projects. The universities which participated were Technische Universität Berlin, Beijing Institute of Technology, Universidade de São Paulo, Chang Gung University, University of Leicester, Université de Toulouse, TianjinUniversity, Kungliga Tekniska högskolan, Karolinska Institutet and University College London. Responses were collected from the location where the universities are located. This helped to collect opinions from around the world, not only confined to Hong Kong. We can distinguish the importance and acceptance of our project in a much larger scale of different places worldwide. </p></font>
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<p>CUHK was honoured to be in cooperation with 10 various different universities to conduct an international questionnaire on synthetic biology and on our respective projects. The universities which participated were Technische Universität Berlin, Beijing Institute of Technology, Universidade de São Paulo, Chang Gung University, University of Leicester, Université de Toulouse, TianjinUniversity, Kungliga Tekniska högskolan, Karolinska Institutet and University College London. Responses were collected from the location where the universities are located. This helped to collect opinions from around the world, not only confined to Hong Kong. We can distinguish the importance and acceptance of our project in a much larger scale of different places worldwide. </p>
 
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<p><font face="Times New Roman" size="6pt">3. UCSF iGEM Team WikiFlicks </p>
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<h2>(3) UCSF iGEM Team WikiFlicks </h2>
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<img src="https://static.igem.org/mediawiki/2015/a/a3/CUHK_Policy_and_Practice_UCSF_iGEM_Team.jpg" width="300px" align="right" style="align-content: space-around; margin: 0 10px">
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For collaboration and to enable people worldwide to further understand synthetic biology and our project, we also worked with the UCSF iGEM Team. Which is a team that includes students from UC Berkeley, San Francisco State University, and recent graduates from Lincoln High School in San Francisco. Through this collaboration, we were able to present our videos on the UCSF iGEM Team’s WikiFlicks. This enable us to spread the message to a much greater number of audience. </p> 
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<p><font face="Times New Roman" size="4pt">For collaboration and to enable people worldwide to further understand synthetic biology and our project, we also worked with the UCSF iGEM Team. Which is a team that includes students from UC Berkeley; San Francisco State University; and recent graduates from Lincoln High School in San Francisco. Through this collaboration, we were able to present our videos on the UCSF iGEM Team’s WikiFlicks. This enable us to spread the message to a much greater number of audience. </p></font>   
 
 
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Latest revision as of 18:18, 11 October 2015

Collaborations

Apart from working on our project, our team also respect the “iGEM values” very much. We believe that through co-operating with other iGEM teams, we can share our passion and assist each other to create better results.



(1) Lab Work and Modelling Collaborations with City University of Hong Kong


For this collaboration, our team members visited each other’s university and presented our projects with the team members and professors. During the visit, we have reached an agreement on collaboration of which we would help them to do characterization on the expression of the lacZY gene. And they helped us to do a part of the modeling for the application part of our project.


CityU team provided us 2 plasmids for the characterization:



1. Pro LacZY

2. Pro LacZ(control)

Theoretically, only the E. coli containing LacZY would absorb ortho-Nitrophenyl-β-galactoside (OPNG) which is a lactose analogue. Then the beta-galactosidase produced by the LacZY E. coli would convert the OPNG into galactose and ortho-nitrophenol, which is yellow in colour and absorbs at 420 nm. Therefore we can measure OD420 to monitor the expression of LacZY.



Procedures:

1. Prepare an overnight culture of LacZ and LacZY in E. coli.

2. Measure the OD420 of the overnight culture and dilute it into ~0.5 OD.

3. Add OPNG solution into the sample.

4. Measure OD420 at 20 min intervals.



========================================================================================================================


(2) International Questionnaire with 10 Different Universities Worldwide



CUHK was honoured to be in cooperation with 10 various different universities to conduct an international questionnaire on synthetic biology and on our respective projects. The universities which participated were Technische Universität Berlin, Beijing Institute of Technology, Universidade de São Paulo, Chang Gung University, University of Leicester, Université de Toulouse, TianjinUniversity, Kungliga Tekniska högskolan, Karolinska Institutet and University College London. Responses were collected from the location where the universities are located. This helped to collect opinions from around the world, not only confined to Hong Kong. We can distinguish the importance and acceptance of our project in a much larger scale of different places worldwide.




========================================================================================================================



(3) UCSF iGEM Team WikiFlicks


For collaboration and to enable people worldwide to further understand synthetic biology and our project, we also worked with the UCSF iGEM Team. Which is a team that includes students from UC Berkeley, San Francisco State University, and recent graduates from Lincoln High School in San Francisco. Through this collaboration, we were able to present our videos on the UCSF iGEM Team’s WikiFlicks. This enable us to spread the message to a much greater number of audience.