Difference between revisions of "Team:Tsinghua/Hardware"

 
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{{Tsinghua}}
 
{{Tsinghua}}
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<html>
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<h2>HARDWARE</h2>
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<title>Hardware</title>
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<h4>Hardware: E-light 1.0</h4>
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<p>  The  E-light 1.0 hardware system has 3 major components: the light-exposure &amp; bacterial  culture system, the controlling circuit and the computer interacting port.<br>
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<h2> Hardware</h2>
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  The  light-exposure &amp; bacterial culture system is based on a 24-well plate  coupled with tri-color LEDs. The controlling circuit utilizes 3 AT89S52-24PU  DIP-40 SCMs (single chip microcomputer) to execute programmed-controlling of  the 24 tri-color LEDs, while the computer interacting port monitors the whole  system through given protocol sequences. The ultimate result is the  programmable operation and real-time monitoring of light-exposure (on both  timing and wave-length) on every single well.<br>
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<p>SHORT DESCRIPTION</p>
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Here below shows the appearance of our device:<br>
 +
<div align="center">
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[[File:Tsinghua_hardware.png|600px]]
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</div>
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  Table below shows the material and components for this delicate device:</p>
 +
 
 +
<table border="1" cellspacing="0" cellpadding="0" align="center">
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  <tr>
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    <td width="553" colspan="2" valign="top"><p align="center">Bill of Materials</p></td>
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  </tr>
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  <tr>
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    <td width="277" valign="top"><p>Materials</p></td>
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    <td width="277" valign="top"><p>Amount</p></td>
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  </tr>
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  <tr>
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    <td width="277" valign="top"><p>IC: AT89S52</p></td>
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    <td width="277" valign="top"><p>3</p></td>
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  </tr>
 +
  <tr>
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    <td width="277" valign="top"><p>IC: STC89C52</p></td>
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    <td width="277" valign="top"><p>1</p></td>
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  </tr>
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  <tr>
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    <td width="277" valign="top"><p>Minimum system    circuit</p></td>
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    <td width="277" valign="top"><p>3</p></td>
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  </tr>
 +
  <tr>
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    <td width="277" valign="top"><p>Common cathode    RGB tri-color LED</p></td>
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    <td width="277" valign="top"><p>24</p></td>
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  </tr>
 +
  <tr>
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    <td width="277" valign="top"><p>8050 triode</p></td>
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    <td width="277" valign="top"><p>24*3</p></td>
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  </tr>
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  <tr>
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    <td width="277" valign="top"><p>1K resistor</p></td>
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    <td width="277" valign="top"><p>24*3</p></td>
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  </tr>
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  <tr>
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    <td width="277" valign="top"><p>USB-RS232</p></td>
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    <td width="277" valign="top"><p>1</p></td>
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  </tr>
 +
  <tr>
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    <td width="277" valign="top"><p>Flat cable    &amp;Ejector plate&amp; Cable</p></td>
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    <td width="277" valign="top"><p>several</p></td>
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  </tr>
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</table>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<div>
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<p>Our collaborative team, Tsinghua-A, has tested our hardware device and they had a great experience. Here links to their feedback:
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   <div align="right">several links including igem</div>
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https://2015.igem.org/Team:Tsinghua-A#collabration</p>
 +
 
 +
   <h4>Software: E-code 1.0</h4>
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<p>  The  E-code 1.0 software system aims to provide convenient commanding for users of  the E-light hardware system. The software provides two operating modes: the  E.coli-code mode is able to convert any given information into light-coded  files, and therefore turn these files into actual light-exposure commands of  the E-light hardware system. With the help of the coding-plasmids from our  CRISPR-Recombinase system, we can eventually store any information into the  E.coli DNA and of course, extract the information later on through sequencing.  The self-code mode provides more flexible input options, enabling users to  program the light-exposure commands manually for every single  bacterial-culture-unit. Thus, combined with our light-switch, the user is able  to gain better control over the bacteria&rsquo;s metabolism pathways.<br>
 +
  After successfully constructing all the systems required and  confirming its efficacy, we can bridge the light-switchable TCS and the  dCas9-recombines system together. In this way, precise gene editing and  information storing can be achieved by utilizing the light system to regulate  the dCas9-recombinase hybrid. <br>
 +
Here below is a simple demonstration of our embedded software in the hardware:</p>
 +
<div align="center">
 +
[[File:Tsinghua_software.gif]]
 
</div>
 
</div>
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<div>Design and Mechanism</div>
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<div class="highlightBox">
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<h2>Hardware Tutorial</h2>
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  <p>Fig and introduction</p>
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<p>We Tsinghua team have recorded a video tutorial for our potential hardware users:</p>
 +
<div align="center">
 +
[[File:Tsinghua_2015.mp4]]
 
</div>
 
</div>
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<div>
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  <p>tutorial</p>
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<html>
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  <p>software</p>
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<div type="container" align="center">
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<p>________________________________________________________________________________________________________________________</p>
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<a href="https://2015.igem.org/"><img src="https://static.igem.org/mediawiki/2015/e/e4/IGEM_2015logo.jpg" height="50px;"></a>
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<a href="https://2015.igem.org/Team:Tsinghua"><img src="https://static.igem.org/mediawiki/2015/c/cf/Tsinghua_TeamLogo.jpg" height="50px;"></a>
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<a href="http://www.tsinghua.edu.cn/en"><img src="https://static.igem.org/mediawiki/2015/5/5c/Tsinghua_thu.jpg" height="50px;"></a>
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<a href="http://life.tsinghua.edu.cn/english/"><img src="https://static.igem.org/mediawiki/2015/4/4c/Tsinghua_SLS.jpg" height="50px;"></a>
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<a href="http://xuetangban.life.tsinghua.edu.cn/"><img src="https://static.igem.org/mediawiki/2015/b/b5/Tsinghua_xuetangban.jpg" height="50px;"></a>
 
</div>
 
</div>
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<p>[logos and links]&lt;nothing should be more than 3 clicks away&gt; </p>
 
 
</html>
 
</html>

Latest revision as of 13:08, 24 October 2015

HARDWARE

Hardware: E-light 1.0

The E-light 1.0 hardware system has 3 major components: the light-exposure & bacterial culture system, the controlling circuit and the computer interacting port.
The light-exposure & bacterial culture system is based on a 24-well plate coupled with tri-color LEDs. The controlling circuit utilizes 3 AT89S52-24PU DIP-40 SCMs (single chip microcomputer) to execute programmed-controlling of the 24 tri-color LEDs, while the computer interacting port monitors the whole system through given protocol sequences. The ultimate result is the programmable operation and real-time monitoring of light-exposure (on both timing and wave-length) on every single well.
Here below shows the appearance of our device:

Tsinghua hardware.png

Table below shows the material and components for this delicate device:

Bill of Materials

Materials

Amount

IC: AT89S52

3

IC: STC89C52

1

Minimum system circuit

3

Common cathode RGB tri-color LED

24

8050 triode

24*3

1K resistor

24*3

USB-RS232

1

Flat cable &Ejector plate& Cable

several

 

Our collaborative team, Tsinghua-A, has tested our hardware device and they had a great experience. Here links to their feedback: https://2015.igem.org/Team:Tsinghua-A#collabration

Software: E-code 1.0

The E-code 1.0 software system aims to provide convenient commanding for users of the E-light hardware system. The software provides two operating modes: the E.coli-code mode is able to convert any given information into light-coded files, and therefore turn these files into actual light-exposure commands of the E-light hardware system. With the help of the coding-plasmids from our CRISPR-Recombinase system, we can eventually store any information into the E.coli DNA and of course, extract the information later on through sequencing. The self-code mode provides more flexible input options, enabling users to program the light-exposure commands manually for every single bacterial-culture-unit. Thus, combined with our light-switch, the user is able to gain better control over the bacteria’s metabolism pathways.
After successfully constructing all the systems required and confirming its efficacy, we can bridge the light-switchable TCS and the dCas9-recombines system together. In this way, precise gene editing and information storing can be achieved by utilizing the light system to regulate the dCas9-recombinase hybrid.
Here below is a simple demonstration of our embedded software in the hardware:

Tsinghua software.gif

Hardware Tutorial

We Tsinghua team have recorded a video tutorial for our potential hardware users:

________________________________________________________________________________________________________________________