Difference between revisions of "Team:Tsinghua/Hardware"

 
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Here below shows the appearance of our device:<br>
 
Here below shows the appearance of our device:<br>
 
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   Table below shows the material and components for this delicate device:</p>
 
   Table below shows the material and components for this delicate device:</p>
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<h2>Hardware Tutorial</h2>
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<p>We Tsinghua team have recorded a video tutorial for our potential hardware users:</p>
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[[File:Tsinghua_2015.mp4]]
 
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Latest revision as of 13:08, 24 October 2015

HARDWARE

Hardware: E-light 1.0

The E-light 1.0 hardware system has 3 major components: the light-exposure & bacterial culture system, the controlling circuit and the computer interacting port.
The light-exposure & bacterial culture system is based on a 24-well plate coupled with tri-color LEDs. The controlling circuit utilizes 3 AT89S52-24PU DIP-40 SCMs (single chip microcomputer) to execute programmed-controlling of the 24 tri-color LEDs, while the computer interacting port monitors the whole system through given protocol sequences. The ultimate result is the programmable operation and real-time monitoring of light-exposure (on both timing and wave-length) on every single well.
Here below shows the appearance of our device:

Tsinghua hardware.png

Table below shows the material and components for this delicate device:

Bill of Materials

Materials

Amount

IC: AT89S52

3

IC: STC89C52

1

Minimum system circuit

3

Common cathode RGB tri-color LED

24

8050 triode

24*3

1K resistor

24*3

USB-RS232

1

Flat cable &Ejector plate& Cable

several

 

Our collaborative team, Tsinghua-A, has tested our hardware device and they had a great experience. Here links to their feedback: https://2015.igem.org/Team:Tsinghua-A#collabration

Software: E-code 1.0

The E-code 1.0 software system aims to provide convenient commanding for users of the E-light hardware system. The software provides two operating modes: the E.coli-code mode is able to convert any given information into light-coded files, and therefore turn these files into actual light-exposure commands of the E-light hardware system. With the help of the coding-plasmids from our CRISPR-Recombinase system, we can eventually store any information into the E.coli DNA and of course, extract the information later on through sequencing. The self-code mode provides more flexible input options, enabling users to program the light-exposure commands manually for every single bacterial-culture-unit. Thus, combined with our light-switch, the user is able to gain better control over the bacteria’s metabolism pathways.
After successfully constructing all the systems required and confirming its efficacy, we can bridge the light-switchable TCS and the dCas9-recombines system together. In this way, precise gene editing and information storing can be achieved by utilizing the light system to regulate the dCas9-recombinase hybrid.
Here below is a simple demonstration of our embedded software in the hardware:

Tsinghua software.gif

Hardware Tutorial

We Tsinghua team have recorded a video tutorial for our potential hardware users:

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