Difference between revisions of "Team:SVCE Chennai/Notebook"

(Prototype team page)
 
 
(15 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
{{SVCE_Chennai}}
 
 
<html>
 
<html>
  
−
<h2>Notebook</h2>
+
<meta name="apple-mobile-web-app-capable" content="yes">
 +
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
  
−
<p> Document the dates you worked on your project.</p>
+
<link rel="stylesheet" type="text/css" href="https://bootswatch.com/flatly/bootstrap.css"/>
 +
 
 +
 
 +
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:SVCE_Chennai/CSS?action=raw&ctype=text/css" />
 +
 
 +
<link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
 +
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.4.0/css/font-awesome.min.css">
 +
 
 +
<nav class="navBarIGEM navbar navbar-default navbar-fixed-top">
 +
 
 +
  <div class="navbar-header">
 +
<button class="navbar-toggle" type="button" data-toggle="collapse" data-target=".js-navbar-collapse">
 +
<span class="sr-only">Toggle navigation</span>
 +
<span class="icon-bar"></span>
 +
<span class="icon-bar"></span>
 +
<span class="icon-bar"></span>
 +
</button>
 +
<a class="navbar-brand" href="https://2015.igem.org/Team:SVCE_Chennai" style="padding-top:0px;"><img src="https://static.igem.org/mediawiki/2015/2/25/MainLogo_Title.png" class="teamLogo" style="    padding-left: 15px;"></a>
 +
</div>
 +
 +
 +
<div class="collapse navbar-collapse js-navbar-collapse">
 +
<ul id="sdt_menu" class="sdt_menu">
 +
            <li>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Results">
 +
<img src="https://static.igem.org/mediawiki/2015/b/b9/MenuImgs_results.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Results</span>
 +
<span class="sdt_descr">Data and Chart</span>
 +
</span>
 +
</a>
 +
                           
 +
</li>
 +
<li>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Gallery">
 +
<img src="https://static.igem.org/mediawiki/2015/c/c2/MenuImgs_mor.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Gallery</span>
 +
<span class="sdt_descr">Click o Click</span>
 +
</span>
 +
</a>
 +
                           
 +
</li>
 +
            <li>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Practices">
 +
<img src="https://static.igem.org/mediawiki/2015/1/17/MenuImgs_parts.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Practices</span>
 +
<span class="sdt_descr">Spread the word</span>
 +
</span>
 +
</a>
 +
               
 +
                    <div class="sdt_box">
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Practices">Human Practices</a>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Collaboration">Collaboration</a>
 +
                        <a href="https://2015.igem.org/Team:SVCE_Chennai/Entrepreneurship">Entrepreneurship</a>
 +
</div>
 +
</li>
 +
           
 +
<li>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Notebook">
 +
<img src="https://static.igem.org/mediawiki/2015/e/e9/MenuImgs_notebook.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Notebook</span>
 +
<span class="sdt_descr">My Calendar</span>
 +
</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
<li>
 +
                    <a href="https://2015.igem.org/Team:SVCE_Chennai/Safety">
 +
<img src="https://static.igem.org/mediawiki/2015/d/d4/MenuImgs_bs.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Safety</span>
 +
<span class="sdt_descr">Safety is my priority</span>
 +
</span>
 +
</a>
 +
</li>
 +
<li>
 +
                              <a href="https://2015.igem.org/Team:SVCE_Chennai/Team">
 +
<img src="https://static.igem.org/mediawiki/2015/3/30/MenuImgs_team.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Team</span>
 +
<span class="sdt_descr">My creators</span>
 +
</span>
 +
</a>
 +
   
 +
                    <div class="sdt_box">
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Team">Members</a>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Brainstorm">Brainstorm</a>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Attributions">Attributions</a>
 +
                       
 +
</div>
 +
</li>
 +
<li>
 +
<a href="https://2015.igem.org/Team:SVCE_Chennai/Project">
 +
<img src="https://static.igem.org/mediawiki/2015/9/9d/MenuImgs1.jpg" alt=""/>
 +
<span class="sdt_active"></span>
 +
<span class="sdt_wrap">
 +
<span class="sdt_link">Project</span>
 +
<span class="sdt_descr">It's all about me</span>
 +
</span>
 +
</a>
 +
             
 +
</li>
 +
</ul>
 +
 +
</div><!-- /.nav-collapse -->
 +
     
 +
</nav>
 +
 
 +
    <div class="container">
 +
        <h2 class="titleStyle">Notebook</h2>
 +
<div class="row stickyNotification">
 +
 
 +
  <p><br />
 +
</p>
 +
 
 +
<p><b>APRIL 2015</b></p>
  
−
<h5>What should this page have?</h5>
 
 
<ul>
 
<ul>
−
<li>Chronological notes of what your team is doing.</li>
+
<li><b>Recruitment of team members</b></li>
−
<li> Brief descriptions of daily important events.</li>
+
<li><b>Setting up of lab with necessary equipments and chemicals</b></li>
−
<li>Pictures of your progress. </li>
+
−
<li>Mention who participated in what task.</li>
+
 
</ul>
 
</ul>
  
 +
<ul>
 +
<li><b>Brainstorming</b></li>
 +
</ul>
  
−
<h4>Inspiration</h4>
+
<p><br />
−
<p>You can see what others teams have done to organize their notes:</p>
+
</p>
  
−
<ul>  
+
<p><b>MAY- MID JUNE 2015</b></p>
−
<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
+
 
−
<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
+
<ul>
−
<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
+
<li><b>Brainstorming </b></li>
−
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
+
<li><b>University exams hence no lab work</b></li>
 
</ul>
 
</ul>
  
−
</div>
+
<p><br />
 +
</p>
 +
 
 +
<p><b>MID JUNE- JULY 2015</b></p>
 +
 
 +
<ul>
 +
<li><b>lab practice sessions </b></li>
 +
<li><b>confirmation of project title</b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>AUGUST 2015</b></p>
 +
 
 +
<p><b>Week 1</b></p>
 +
 
 +
<ul>
 +
<li><b>Submitted safety forms and also regarding our project forms.</b></li>
 +
</ul>
 +
 
 +
<ul>
 +
<li><b>Preparation of LB broth and LB Agar and plates were poured.</b></li>
 +
<li><b>Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.</b></li>
 +
<li><b>Abstract and title of our project was uploaded.</b></li>
 +
</ul>
 +
 
 +
<ul>
 +
<li><b>Prepared chemicals and buffers for plasmid isolation.</b></li>
 +
<li><b>FtsZ was innoculated in LB broth.</b></li>
 +
<li><b>Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.</b></li>
 +
</ul>
 +
 
 +
<ul>
 +
<li><b>Sequences for gBlocks were constructed for ordering.</b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>Week 2:</b></p>
 +
 
 +
<ul>
 +
<li><b>Plasmid isolation was performed for protocol optimisation.</b></li>
 +
</ul>
 +
 
 +
<ul>
 +
<li><b>Result was negative and so the isolation was performed again.</b></li>
 +
<li><b>Elution of DNA was done but results were not satisfactory.</b></li>
 +
<li><b>Media (SOB and SOC) were prepared for competent cell preparation.</b></li>
 +
<li><b>Positive results were obtained for elution of DNA and protocol was optimised.</b></li>
 +
<li><b>Innoculated BL 21 in SOB.</b></li>
 +
<li><b>Presentation and questionnaire based on our project was prepared to educate the students in different colleges.</b></li>
 +
<li><b>Logo designing was initiated.</b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>Week 3:</b></p>
 +
 
 +
<ul>
 +
<li><b>Growth curve for Pseudomonas aeruginosa was done and results were analysed.</b></li>
 +
<li><b>Growth curve for BL 21 was done.</b></li>
 +
<li><b>Plasmid isolation of pET24a was performed.</b></li>
 +
<li><b>Competent cell of BL 21 was made and protocol was optimised.</b></li>
 +
<li><b>Seed stock for BL 21 competent cell was made.</b></li>
 +
<li><b>Transformation was performed.</b></li>
 +
<li><b>Primers required for our project were designed and ordered.</b></li>
 +
<li><b>For Human Practise questionnaire for hospitals were prepared. </b></li>
 +
<li><b>Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.</b></li>
 +
<li><b>Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance. </b></li>
 +
<li><b>Logo design was reviewed and changes were made.</b></li>
 +
<li><b>Approached various funding agencies.</b></li>
 +
<li><b>Collaboration with Uppsala was confirmed.</b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>Week 4:</b></p>
 +
 
 +
<ul>
 +
<li><b>BL21 was subcultured.</b></li>
 +
<li><b>Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.</b></li>
 +
<li><b>Chloramphenicol plates were prepared for pSB1C3 amplification.</b></li>
 +
<li><b>Methicillin Resistant Staphylococcus aureus were obtained.</b></li>
 +
<li><b>Final safety forms were filled.</b></li>
 +
<li><b>First step was taken for wiki page and its theme was discussed.</b></li>
 +
<li><b>Responses for various questionnaire was collected and a statistical analysis was carried out.</b></li>
 +
<li><b>Results were presented to the faculty members of our department.</b></li>
 +
<li><b>Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.</b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>SEPTEMBER 2015</b></p>
 +
 
 +
<p><b> Week 1:</b></p>
 +
 
 +
<ul>
 +
<li><b>gBlocks of ftsZ was obtained. </b></li>
 +
<li><b>PCR amplification of the ftsZ was done. </b></li>
 +
<li><b>Results were found to be negative.</b></li>
 +
<li><b>Hence they were troubleshooted.</b></li>
 +
<li><b>Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.</b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>Week 2:</b></p>
 +
 
 +
<ul>
 +
<li><b>gBlocks of thuricin was obtained.</b></li>
 +
<li><b>PCR amplification of the gene was done.</b></li>
 +
<li><b>This amplified gene was cloned into pSB1C3.</b></li>
 +
<li><b>Growth curve for Staphylococcus aureus was done.</b></li>
 +
<li><b>Human practices team presented in Madha Engineering College and SBOA. </b></li>
 +
</ul>
 +
 
 +
<p><br />
 +
</p>
 +
 
 +
<p><b>Week 3:</b></p>
 +
 
 +
<ul>
 +
<li><b>Got bactofencin gBlocks and PCR amplification was done.</b></li>
 +
<li><b>Then cloning was done in pSB1C3.</b></li>
 +
<li><b>Cloned Thuricin and Bactofencin were submitted as parts.</b></li>
 +
<li><b>Public interview regarding antibiotic resistance was taken.</b></li>
 +
<li><b>Descriptions were uploaded in wiki page.</b></li>
 +
</ul>
 +
 
 +
  </div>
 +
    </div>
 +
  <style>
 +
      #magazine{
 +
    width: 800px;
 +
    height: 400px;
 +
          margin: 0 auto;
 +
}
 +
#magazine .turn-page{
 +
    background-color:#ccc;
 +
      }</style>
 +
   
 +
   
 +
 
 +
    <footer class="footer " style="position:fixed">
 +
      <div class="container">
 +
      <div class="row">
 +
        <div class="col-md-4"><img src="https://static.igem.org/mediawiki/2015/3/3e/Minio.png" width=50>
 +
            <span class="speech" data-toggle="tooltip" title="Did you know! you can speak with me! Ask me questions like: Who are you?">Lets talk!
 +
              <div class="fa fa-microphone microphone-icon"></div>
 +
          </span>
 +
        </div>
 +
         
 +
        <div class="col-md-4" style="float:right;  border-left: 1px solid #60C3EB; transform:skew(">
 +
 
 +
        <img src="https://upload.wikimedia.org/wikipedia/en/7/71/SVCE_logo.png" width=50>
 +
<p style=" display: inline; font-size: 14px;  transform: skew(-30deg); ">Sri Venkateswara College of Engineering, Chennai</p>
 +
      </div>
 +
      </div>
 +
      </div>
 +
    </footer>
 +
 
 +
 
 +
<script type="text/javascript" src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/js/bootstrap.min.js"></script>
 +
<script type="text/javascript" src="https://2015.igem.org/Team:SVCE_Chennai/Javascript?action=raw&ctype=text/javascript"></script>
 +
<script src="http://www.mattboldt.com/demos/typed-js/js/typed.custom.js"></script>
 +
<script src="https://cdnjs.cloudflare.com/ajax/libs/annyang/2.0.0/annyang.min.js"></script>
 +
<script src="https://cdnjs.cloudflare.com/ajax/libs/jquery-easing/1.3/jquery.easing.min.js"></script>
 +
<script src="http://www.turnjs.com/lib/turn.min.js"></script>
 +
<script>
 +
    $('#magazine').turn({gradients: true, acceleration: true});
 +
    </script>
 +
   
 
</html>
 
</html>

Latest revision as of 12:32, 30 October 2015

Notebook


APRIL 2015

  • Recruitment of team members
  • Setting up of lab with necessary equipments and chemicals
  • Brainstorming


MAY- MID JUNE 2015

  • Brainstorming
  • University exams hence no lab work


MID JUNE- JULY 2015

  • lab practice sessions
  • confirmation of project title


AUGUST 2015

Week 1

  • Submitted safety forms and also regarding our project forms.
  • Preparation of LB broth and LB Agar and plates were poured.
  • Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.
  • Abstract and title of our project was uploaded.
  • Prepared chemicals and buffers for plasmid isolation.
  • FtsZ was innoculated in LB broth.
  • Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.
  • Sequences for gBlocks were constructed for ordering.


Week 2:

  • Plasmid isolation was performed for protocol optimisation.
  • Result was negative and so the isolation was performed again.
  • Elution of DNA was done but results were not satisfactory.
  • Media (SOB and SOC) were prepared for competent cell preparation.
  • Positive results were obtained for elution of DNA and protocol was optimised.
  • Innoculated BL 21 in SOB.
  • Presentation and questionnaire based on our project was prepared to educate the students in different colleges.
  • Logo designing was initiated.


Week 3:

  • Growth curve for Pseudomonas aeruginosa was done and results were analysed.
  • Growth curve for BL 21 was done.
  • Plasmid isolation of pET24a was performed.
  • Competent cell of BL 21 was made and protocol was optimised.
  • Seed stock for BL 21 competent cell was made.
  • Transformation was performed.
  • Primers required for our project were designed and ordered.
  • For Human Practise questionnaire for hospitals were prepared.
  • Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.
  • Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance.
  • Logo design was reviewed and changes were made.
  • Approached various funding agencies.
  • Collaboration with Uppsala was confirmed.


Week 4:

  • BL21 was subcultured.
  • Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.
  • Chloramphenicol plates were prepared for pSB1C3 amplification.
  • Methicillin Resistant Staphylococcus aureus were obtained.
  • Final safety forms were filled.
  • First step was taken for wiki page and its theme was discussed.
  • Responses for various questionnaire was collected and a statistical analysis was carried out.
  • Results were presented to the faculty members of our department.
  • Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.


SEPTEMBER 2015

Week 1:

  • gBlocks of ftsZ was obtained.
  • PCR amplification of the ftsZ was done.
  • Results were found to be negative.
  • Hence they were troubleshooted.
  • Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.


Week 2:

  • gBlocks of thuricin was obtained.
  • PCR amplification of the gene was done.
  • This amplified gene was cloned into pSB1C3.
  • Growth curve for Staphylococcus aureus was done.
  • Human practices team presented in Madha Engineering College and SBOA.


Week 3:

  • Got bactofencin gBlocks and PCR amplification was done.
  • Then cloning was done in pSB1C3.
  • Cloned Thuricin and Bactofencin were submitted as parts.
  • Public interview regarding antibiotic resistance was taken.
  • Descriptions were uploaded in wiki page.
Lets talk!

Sri Venkateswara College of Engineering, Chennai