Difference between revisions of "Team:SVCE Chennai/Notebook"
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<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
</button> | </button> | ||
− | <a class="navbar-brand" href="https://2015.igem.org/Team:SVCE_Chennai" style="padding-top:0px;"><img src="https://static.igem.org/mediawiki/2015/2/25/MainLogo_Title.png" class="teamLogo"></a> | + | <a class="navbar-brand" href="https://2015.igem.org/Team:SVCE_Chennai" style="padding-top:0px;"><img src="https://static.igem.org/mediawiki/2015/2/25/MainLogo_Title.png" class="teamLogo" style=" padding-left: 15px;"></a> |
</div> | </div> | ||
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<div class="collapse navbar-collapse js-navbar-collapse"> | <div class="collapse navbar-collapse js-navbar-collapse"> | ||
<ul id="sdt_menu" class="sdt_menu"> | <ul id="sdt_menu" class="sdt_menu"> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:SVCE_Chennai/Results"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/b/b9/MenuImgs_results.jpg" alt=""/> | ||
+ | <span class="sdt_active"></span> | ||
+ | <span class="sdt_wrap"> | ||
+ | <span class="sdt_link">Results</span> | ||
+ | <span class="sdt_descr">Data and Chart</span> | ||
+ | </span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
<li> | <li> | ||
<a href="https://2015.igem.org/Team:SVCE_Chennai/Gallery"> | <a href="https://2015.igem.org/Team:SVCE_Chennai/Gallery"> | ||
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<div class="sdt_box"> | <div class="sdt_box"> | ||
<a href="https://2015.igem.org/Team:SVCE_Chennai/Practices">Human Practices</a> | <a href="https://2015.igem.org/Team:SVCE_Chennai/Practices">Human Practices</a> | ||
− | <a href="https://2015.igem.org/Team:SVCE_Chennai/ | + | <a href="https://2015.igem.org/Team:SVCE_Chennai/Collaboration">Collaboration</a> |
+ | <a href="https://2015.igem.org/Team:SVCE_Chennai/Entrepreneurship">Entrepreneurship</a> | ||
</div> | </div> | ||
</li> | </li> | ||
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<div class="row stickyNotification"> | <div class="row stickyNotification"> | ||
− | + | <p><br /> | |
− | + | </p> | |
− | + | ||
− | + | <p><b>APRIL 2015</b></p> | |
− | </ | + | |
− | + | <ul> | |
+ | <li><b>Recruitment of team members</b></li> | ||
+ | <li><b>Setting up of lab with necessary equipments and chemicals</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Brainstorming</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>MAY- MID JUNE 2015</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Brainstorming </b></li> | ||
+ | <li><b>University exams hence no lab work</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>MID JUNE- JULY 2015</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>lab practice sessions </b></li> | ||
+ | <li><b>confirmation of project title</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>AUGUST 2015</b></p> | ||
+ | |||
+ | <p><b>Week 1</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Submitted safety forms and also regarding our project forms.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Preparation of LB broth and LB Agar and plates were poured.</b></li> | ||
+ | <li><b>Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.</b></li> | ||
+ | <li><b>Abstract and title of our project was uploaded.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Prepared chemicals and buffers for plasmid isolation.</b></li> | ||
+ | <li><b>FtsZ was innoculated in LB broth.</b></li> | ||
+ | <li><b>Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Sequences for gBlocks were constructed for ordering.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>Week 2:</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Plasmid isolation was performed for protocol optimisation.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Result was negative and so the isolation was performed again.</b></li> | ||
+ | <li><b>Elution of DNA was done but results were not satisfactory.</b></li> | ||
+ | <li><b>Media (SOB and SOC) were prepared for competent cell preparation.</b></li> | ||
+ | <li><b>Positive results were obtained for elution of DNA and protocol was optimised.</b></li> | ||
+ | <li><b>Innoculated BL 21 in SOB.</b></li> | ||
+ | <li><b>Presentation and questionnaire based on our project was prepared to educate the students in different colleges.</b></li> | ||
+ | <li><b>Logo designing was initiated.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>Week 3:</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Growth curve for Pseudomonas aeruginosa was done and results were analysed.</b></li> | ||
+ | <li><b>Growth curve for BL 21 was done.</b></li> | ||
+ | <li><b>Plasmid isolation of pET24a was performed.</b></li> | ||
+ | <li><b>Competent cell of BL 21 was made and protocol was optimised.</b></li> | ||
+ | <li><b>Seed stock for BL 21 competent cell was made.</b></li> | ||
+ | <li><b>Transformation was performed.</b></li> | ||
+ | <li><b>Primers required for our project were designed and ordered.</b></li> | ||
+ | <li><b>For Human Practise questionnaire for hospitals were prepared. </b></li> | ||
+ | <li><b>Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.</b></li> | ||
+ | <li><b>Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance. </b></li> | ||
+ | <li><b>Logo design was reviewed and changes were made.</b></li> | ||
+ | <li><b>Approached various funding agencies.</b></li> | ||
+ | <li><b>Collaboration with Uppsala was confirmed.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>Week 4:</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>BL21 was subcultured.</b></li> | ||
+ | <li><b>Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.</b></li> | ||
+ | <li><b>Chloramphenicol plates were prepared for pSB1C3 amplification.</b></li> | ||
+ | <li><b>Methicillin Resistant Staphylococcus aureus were obtained.</b></li> | ||
+ | <li><b>Final safety forms were filled.</b></li> | ||
+ | <li><b>First step was taken for wiki page and its theme was discussed.</b></li> | ||
+ | <li><b>Responses for various questionnaire was collected and a statistical analysis was carried out.</b></li> | ||
+ | <li><b>Results were presented to the faculty members of our department.</b></li> | ||
+ | <li><b>Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>SEPTEMBER 2015</b></p> | ||
+ | |||
+ | <p><b> Week 1:</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>gBlocks of ftsZ was obtained. </b></li> | ||
+ | <li><b>PCR amplification of the ftsZ was done. </b></li> | ||
+ | <li><b>Results were found to be negative.</b></li> | ||
+ | <li><b>Hence they were troubleshooted.</b></li> | ||
+ | <li><b>Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.</b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>Week 2:</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>gBlocks of thuricin was obtained.</b></li> | ||
+ | <li><b>PCR amplification of the gene was done.</b></li> | ||
+ | <li><b>This amplified gene was cloned into pSB1C3.</b></li> | ||
+ | <li><b>Growth curve for Staphylococcus aureus was done.</b></li> | ||
+ | <li><b>Human practices team presented in Madha Engineering College and SBOA. </b></li> | ||
+ | </ul> | ||
+ | |||
+ | <p><br /> | ||
+ | </p> | ||
+ | |||
+ | <p><b>Week 3:</b></p> | ||
+ | |||
+ | <ul> | ||
+ | <li><b>Got bactofencin gBlocks and PCR amplification was done.</b></li> | ||
+ | <li><b>Then cloning was done in pSB1C3.</b></li> | ||
+ | <li><b>Cloned Thuricin and Bactofencin were submitted as parts.</b></li> | ||
+ | <li><b>Public interview regarding antibiotic resistance was taken.</b></li> | ||
+ | <li><b>Descriptions were uploaded in wiki page.</b></li> | ||
+ | </ul> | ||
+ | |||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 12:32, 30 October 2015
Notebook
APRIL 2015
- Recruitment of team members
- Setting up of lab with necessary equipments and chemicals
- Brainstorming
MAY- MID JUNE 2015
- Brainstorming
- University exams hence no lab work
MID JUNE- JULY 2015
- lab practice sessions
- confirmation of project title
AUGUST 2015
Week 1
- Submitted safety forms and also regarding our project forms.
- Preparation of LB broth and LB Agar and plates were poured.
- Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.
- Abstract and title of our project was uploaded.
- Prepared chemicals and buffers for plasmid isolation.
- FtsZ was innoculated in LB broth.
- Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.
- Sequences for gBlocks were constructed for ordering.
Week 2:
- Plasmid isolation was performed for protocol optimisation.
- Result was negative and so the isolation was performed again.
- Elution of DNA was done but results were not satisfactory.
- Media (SOB and SOC) were prepared for competent cell preparation.
- Positive results were obtained for elution of DNA and protocol was optimised.
- Innoculated BL 21 in SOB.
- Presentation and questionnaire based on our project was prepared to educate the students in different colleges.
- Logo designing was initiated.
Week 3:
- Growth curve for Pseudomonas aeruginosa was done and results were analysed.
- Growth curve for BL 21 was done.
- Plasmid isolation of pET24a was performed.
- Competent cell of BL 21 was made and protocol was optimised.
- Seed stock for BL 21 competent cell was made.
- Transformation was performed.
- Primers required for our project were designed and ordered.
- For Human Practise questionnaire for hospitals were prepared.
- Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.
- Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance.
- Logo design was reviewed and changes were made.
- Approached various funding agencies.
- Collaboration with Uppsala was confirmed.
Week 4:
- BL21 was subcultured.
- Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.
- Chloramphenicol plates were prepared for pSB1C3 amplification.
- Methicillin Resistant Staphylococcus aureus were obtained.
- Final safety forms were filled.
- First step was taken for wiki page and its theme was discussed.
- Responses for various questionnaire was collected and a statistical analysis was carried out.
- Results were presented to the faculty members of our department.
- Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.
SEPTEMBER 2015
Week 1:
- gBlocks of ftsZ was obtained.
- PCR amplification of the ftsZ was done.
- Results were found to be negative.
- Hence they were troubleshooted.
- Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.
Week 2:
- gBlocks of thuricin was obtained.
- PCR amplification of the gene was done.
- This amplified gene was cloned into pSB1C3.
- Growth curve for Staphylococcus aureus was done.
- Human practices team presented in Madha Engineering College and SBOA.
Week 3:
- Got bactofencin gBlocks and PCR amplification was done.
- Then cloning was done in pSB1C3.
- Cloned Thuricin and Bactofencin were submitted as parts.
- Public interview regarding antibiotic resistance was taken.
- Descriptions were uploaded in wiki page.