Difference between revisions of "Team:Georgia State/Description"

 
(6 intermediate revisions by 2 users not shown)
Line 16: Line 16:
  
 
     <!-- CSS -->
 
     <!-- CSS -->
−
     <link href="https://2015.igem.org/Template:Georgia_State/Style.css?action=raw&ctype=text/css" rel="stylesheet" type="text/css" />
+
     <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Georgia_State/CSS?action=raw&ctype=text/css" />
−
   
+
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Georgia_State/CSS1?action=raw&ctype=text/css" />
−
 
+
 
</head>
 
</head>
  
Line 39: Line 38:
 
                 <!-- logo -->
 
                 <!-- logo -->
 
                 <div class="logo">
 
                 <div class="logo">
−
                     <a href="#">
+
                     <a href="https://2015.igem.org/Team:Georgia_State">
−
                        <img class="l-black" src="https://static.igem.org/mediawiki/2015/a/a8/LogoBlack_GSUiGem.png" />
+
                         <img style="margin-top: -10px;" class="l-black" src="https://static.igem.org/mediawiki/2015/a/a8/LogoBlack_GSUiGem.png" />
−
                         <img class="l-white" src="https://static.igem.org/mediawiki/2015/e/e1/WhiteLogo_GSUiGem.png" />  
+
 
                     </a>
 
                     </a>
 
                 </div>
 
                 </div>
Line 48: Line 46:
 
                  
 
                  
  
−
                <!-- Navigation Menu -->
+
<!-- Navigation Menu -->
 
                 <nav class="navigation singlepage-nav">
 
                 <nav class="navigation singlepage-nav">
 
                     <ul>
 
                     <ul>
 
                         <li>
 
                         <li>
−
                             <a class="current-ele" href="#intro">Home</a>
+
                             <a href="https://2015.igem.org/Team:Georgia_State/Team">Team</a>
−
                        </li>
+
−
<li>
+
−
                            <a href="#Team">Team</a>
+
 
                         </li>
 
                         </li>
 
 
 
                         <li>
 
                         <li>
−
                             <a href="#Project">Project</a>
+
                             <a href="https://2015.igem.org/Team:Georgia_State/Project">Project</a>
 
<ul>
 
<ul>
 
<li>
 
<li>
−
  <a href="https://2015.igem.org/Team:Georgia_State/Attributions">Attributions</a>
+
  <a href="https://2015.igem.org/Team:Georgia_State/Project#Thing1">Mambalgin</a>
 
</li>
 
</li>
 
<li>
 
<li>
−
  <a href="https://2015.igem.org/Team:Georgia_State/Practices">Practices</a>
+
  <a href="https://2015.igem.org/Team:Georgia_State/Project#Thing2">CBDA Synthase</a>  
−
<ul>
+
−
<li>
+
−
<a href="https://2015.igem.org/Team:Georgia_State/AtlSci">Atlanta Science Festival</a>
+
−
</li>
+
  
−
<li>
 
−
<a href="https://2015.igem.org/Team:Georgia_State/legislative">Georgia Capitol</a>
 
−
</li>
 
−
 
−
</ul>
 
 
</li>
 
</li>
 
<li>
 
<li>
−
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations">Collaborations</a>
+
  <a href="https://2015.igem.org/Team:Georgia_State/Parts">Parts Submitted</a>  
−
<ul>
+
−
<li>
+
−
<a href="https://2015.igem.org/Team:Georgia_State/AtlSci">Atlanta Science Festival</a>
+
−
</li>
+
−
</ul>
+
  
 
</li>
 
</li>
−
 
 
<li>
 
<li>
−
<a href="https://2015.igem.org/Team:Georgia_State/Description">Description</a>
+
  <a href="https://2015.igem.org/Team:Georgia_State/Description">Part Improvement</a>  
−
+
 
−
</li>
+
−
<li>
+
−
<a href="https://2015.igem.org/Team:Georgia_State/Design">Design</a>
+
 
</li>
 
</li>
 
</ul>
 
</ul>
−
                        </li>
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Notebook">Notebook</a>
−
                       
+
</li>
−
<li>
+
 
−
<a href="https://2015.igem.org/Team:Georgia_State/ImageGallery">Image Gallery</a>
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Practices">Practices</a>
−
                         </li>
+
<ul>
−
                     
+
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing1">Atlanta Science Festival</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing2">Six Flags Education Day</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing3">Legislative Outreach</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing4">Atlanta Botanical Gardens</a>
 +
</li>
 +
</ul>
 +
<li> <a href="https://2015.igem.org/Team:Georgia_State/Collaborations">Collaborations</a>
 +
<ul>
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations#ASF">Atlanta Science Festival</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations#Thing2">Project JBroth</a>
 +
</li>
 +
</ul>
 +
 
 +
<li> <a href="https://2015.igem.org/Team:Georgia_State/Safety">Safety</a>
 +
                        </li>
 +
<li> <a href="https://2015.igem.org/Team:Georgia_State/Attributions">Attributions</a>
 +
</li> 
 +
                         <li> <a href="https://2015.igem.org/Team:Georgia_State/ImageGallery">Images</a>
 +
                        </li>
 +
                   
 +
                     
 
                         <li>
 
                         <li>
−
                             <a href="#contact-info">Contact</a>
+
                             <a href="https://2015.igem.org/Team:Georgia_State#contact-info">Contact</a>
−
                        </li>  
+
                        </li>  
 +
 
 
                     </ul>
 
                     </ul>
 
                 </nav>
 
                 </nav>
−
                <!--End Navigation Menu -->
+
<!--End Navigation Menu -->
 +
 
  
  
Line 129: Line 142:
 
         <div class="clearfix"></div>
 
         <div class="clearfix"></div>
 
         <!-- End Intro Section -->
 
         <!-- End Intro Section -->
 +
 +
<!--About Section-->
 +
        <section id="about" class="wow fadeIn ptb ptb-sm-80" style="background-color: white;">
 +
            <div class="container">
 +
                <div class="row text-center">
 +
                    <div class="col-md-12">
 +
                        <h3 class="h4" style="text-align: center;color: black;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1110003">Mambalgin </h3>
 +
                        <div class="spacer-15"></div>
 +
                        <p class="lead"  style="color: black;" ><a class="tipped" data-title="Abstract" data-tipper-options='{"direction":"top"}'><b>For the design, we took the coding sequence for mambalgin, removed the TAG stop codon, and added RFC 25 prefix and suffix in Snapgene software to visualize. RFC 25 was used because we were anticipating the use of this part in creating fusion proteins. Additional nucleotides were added before the biobrick prefix and after the biobrick suffix to enable more efficient restriction enzyme cutting at EcoRI and PstI sites. The mambalgin construct was then ordered from IDT and ligated into pSB1C3 backbone. Using the pGAPzα expression system provided by ThermoFisher Scientific, the GSU team has been working to express the protein in Pichia Pastoris. The pGAPZα vector includes the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter, an alpha secretory signal peptide, and Myc and 6x His epitopes. The MCS, between the alpha secretion signal and epitopes, consists of restriction sites which will enable insertion of mambalgin. In-frame insertion into the MCS of pGAPza will be possible after using PCR to modify the construct - removing the RFC 25 prefix and suffix and adding restriction sites and nucleotides. </p>
 +
                    </div>
 +
                </div>
 +
                   
 +
            </div>
 +
        </section>
 +
        <!-- End About Section-->
 +
 +
 
</body>
 
</body>
 +
 
 
     <!-- JS -->  
 
     <!-- JS -->  
  
−
<script type="text/javascript" src="https://2015.igem.org/Template:Georgia_State/Javascript?action=raw&c type=text/javascript"></script>
+
<script type="text/javascript" src="https://2015.igem.org/Template:Georgia_State/Javascript?
−
    <script src="js/jquery-1.11.2.min.js" type="text/javascript"></script>
+
action=raw&ctype=text/javascript"></script>
−
   
+
−
   
+
−
     
+
−
 
+
−
    <script type="text/javascript">
+
−
        //  Onepage Nav Elements
+
−
        $('.singlepage-nav').singlePageNav({
+
−
            offset: 0,
+
−
            filter: ':not(.nav-external)',
+
−
            updateHash: true,
+
−
            currentClass: 'current-ele',
+
−
            easing: 'swing',
+
−
            speed: 750,
+
−
            beforeStart: function () {
+
−
                console.log('begin scrolling');
+
−
            },
+
−
            onComplete: function () {
+
−
                console.log('done scrolling');
+
−
            }
+
−
        });
+
−
    </script>
+
 
</html>
 
</html>

Latest revision as of 17:56, 8 November 2015

IGEM Team:GSU


Design


Mambalgin

For the design, we took the coding sequence for mambalgin, removed the TAG stop codon, and added RFC 25 prefix and suffix in Snapgene software to visualize. RFC 25 was used because we were anticipating the use of this part in creating fusion proteins. Additional nucleotides were added before the biobrick prefix and after the biobrick suffix to enable more efficient restriction enzyme cutting at EcoRI and PstI sites. The mambalgin construct was then ordered from IDT and ligated into pSB1C3 backbone. Using the pGAPzα expression system provided by ThermoFisher Scientific, the GSU team has been working to express the protein in Pichia Pastoris. The pGAPZα vector includes the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter, an alpha secretory signal peptide, and Myc and 6x His epitopes. The MCS, between the alpha secretion signal and epitopes, consists of restriction sites which will enable insertion of mambalgin. In-frame insertion into the MCS of pGAPza will be possible after using PCR to modify the construct - removing the RFC 25 prefix and suffix and adding restriction sites and nucleotides.