Difference between revisions of "Team:DTU-Denmark/Project/Overview"

 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
{{Team:DTU-Denmark/header}}
+
<html>
 +
  <head>
 +
    <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 +
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
  
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css">
 +
      <link rel=stylesheet href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css">
  
−
<div class="jumbotron hero" style="background-image: url(https://2015.igem.org<app.models.UploadedFile object at 0x7ff51422db90>)">
+
    <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
 +
    <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
 +
    <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
 +
    <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
 +
 
 +
    <!-- MathJax (LaTeX for the web) -->
 +
    <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
 +
   
 +
  </head>
 +
  <body data-spy="scroll" data-target="#scrollspy" data-offset="90">
 +
    <div id="fb-root"></div>
 +
    <script>(function(d, s, id) {
 +
      var js, fjs = d.getElementsByTagName(s)[0];
 +
      if (d.getElementById(id)) return;
 +
      js = d.createElement(s); js.id = id;
 +
      js.src = "//connect.facebook.net/da_DK/all.js#xfbml=1&version=v2.4";
 +
      fjs.parentNode.insertBefore(js, fjs);
 +
    }(document, 'script', 'facebook-jssdk'));</script>
 +
    <nav class="navbar navbar-default navbar-fixed-top" role="navigation">
 +
      <!-- Brand and toggle get grouped for better mobile display -->
 +
      <div class="container-fluid">
 +
        <div class="navbar-header">
 +
          <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
 +
            <span class="sr-only">Toggle navigation</span>
 +
            <i class="fa fa-bars"></i>
 +
          </button>
 +
          <a class="navbar-brand navimg" href="#"><img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png"></a>
 +
        </div>
 +
        <!-- Collect the nav links, forms, and other content for toggling -->
 +
        <div class="collapse navbar-collapse" id="nav-collapse">
 +
          <ul class="nav navbar-nav">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark"
 +
                  >Home
 +
                  </a></li>
 +
                <li
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                >
 +
                <a href=""
 +
                  class="dropdown-toggle"
 +
                  data-toggle="dropdown"
 +
                  role="button"
 +
                  aria-expanded="false">Team and Attributions
 +
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Team"
 +
                  >Team
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Attributions"
 +
                  >Attributions
 +
                  </a></li></ul></li>
 +
                <li
 +
               
 +
                   
 +
                      class="active"
 +
                   
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                >
 +
                <a href=""
 +
                  class="dropdown-toggle"
 +
                  data-toggle="dropdown"
 +
                  role="button"
 +
                  aria-expanded="false">Project
 +
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li class="active">
 +
                <a href="/Team:DTU-Denmark/Project/Overview"
 +
                  >Overview
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Background"
 +
                  >Background
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/MAGE"
 +
                  >MAGE subtilis
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Tyrocidine"
 +
                  >Tyrocidine
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Chip"
 +
                  >Lab-on-a-disc
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Inteins"
 +
                  >Inteins
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Detection"
 +
                  >Detection of NRP
 +
                  </a></li></ul></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Practices"
 +
                  >Human Practices
 +
                  </a></li>
 +
                <li
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                >
 +
                <a href=""
 +
                  class="dropdown-toggle"
 +
                  data-toggle="dropdown"
 +
                  role="button"
 +
                  aria-expanded="false">Parts Collection
 +
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Parts"
 +
                  >Parts
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Description"
 +
                  >Characterisation of xylR
 +
                  </a></li></ul></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Journal"
 +
                  >Journal
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Software"
 +
                  >Software
 +
                  </a></li>
 +
                <li
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                   
 +
               
 +
                >
 +
                <a href=""
 +
                  class="dropdown-toggle"
 +
                  data-toggle="dropdown"
 +
                  role="button"
 +
                  aria-expanded="false">Achievements
 +
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Achievements"
 +
                  >Key Achievements
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Collaborations"
 +
                  >Collaborations
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Judging_Form"
 +
                  >Judging Form
 +
                  </a></li></ul></li>
 +
            <li class="hidden-xs">
 +
              <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
 +
                <i class="fa fa-facebook"></i><span class="visible-xs"> Follow us on Facebook</span>
 +
              </a>
 +
            </li>
 +
            <li class="visible-xs"><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
 +
            <li class="hidden-xs">
 +
              <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
 +
                <i class="fa fa-twitter"></i>
 +
              </a>
 +
            </li>
 +
            <li class="visible-xs"><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
 +
            <li class="hidden-lg">
 +
              <a href="https://igem.org" target="_blank" class="navimg">
 +
                <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
 +
              </a>
 +
            </li>
 +
          </ul>
 +
        </div><!-- /.navbar-collapse -->
 +
      </div>
 +
    </nav>
 +
 
 +
 
 +
<div class="jumbotron hero" style="background-image: url(https://static.igem.org/mediawiki/2015/0/0b/DTU-Denmark_hero.jpg)">
 
       <div class="igem-logo visible-lg">
 
       <div class="igem-logo visible-lg">
 
         <a href="https://igem.org" target="_blank">
 
         <a href="https://igem.org" target="_blank">
−
           <img src="https://2015.igem.org<app.models.UploadedFile object at 0x7ff51421a810>">
+
           <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
 
         </a>
 
         </a>
 
       </div>
 
       </div>
Line 57: Line 258:
 
       <p>Nonribosomal peptide synthases (NRPSs) are large multimodular enzymes that synthesize nonribosomal peptides, which are short bioactive peptides with a broad range of functions, including antibiotics, immunosuppressants and anticancer drugs.</p>
 
       <p>Nonribosomal peptide synthases (NRPSs) are large multimodular enzymes that synthesize nonribosomal peptides, which are short bioactive peptides with a broad range of functions, including antibiotics, immunosuppressants and anticancer drugs.</p>
  
−
<div class="overview-readmore"><a href="/Team:DTU-Denmark/Project/Background">Read more</a></div>
+
<a href="/Team:DTU-Denmark/Project/Background">Read more</a>
  
 
   </div>
 
   </div>
Line 92: Line 293:
 
      
 
      
 
     <div class="col-md-4">
 
     <div class="col-md-4">
−
       <img src="https://2015.igem.org<app.models.UploadedFile object at 0x7ff5141f0050>">
+
       <img src="https://static.igem.org/mediawiki/2015/7/7b/DTU-Denmark_cycle.png">
 
     </div>
 
     </div>
 
      
 
      
Line 142: Line 343:
 
</div>
 
</div>
  
−
    {{Team:DTU-Denmark/footer}}
+
<footer class="container">
 +
      <div class="row">
 +
        <div class="col-md-2 col-md-offset-2">
 +
          <a href="http://www.dtu.dk" target="_blank">
 +
            <img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png" height="130px">
 +
          </a>
 +
        </div>
 +
        <div class="col-md-4">
 +
          <address>
 +
            <strong>Technical University of Denmark</strong><br>
 +
            Department of Systems Biology <br>
 +
            Søltofts Plads 221 <br>
 +
            2800 Kgs. Lyngby<br>
 +
            Denmark<br>
 +
            <abbr title="Phone">P:</abbr> +45 45 25 25 25<br>
 +
            <abbr title="Mail">M:</abbr> <a href="mailto:dtu-igem-2015@googlegroups.com">dtu-igem-2015@googlegroups.com</a>
 +
          </address>
 +
        </div>
 +
        <div class="col-md-2">
 +
          <a href="https://igem.org" style="float:left" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png" height="90px">
 +
          </a>
 +
        </div>
 +
      </div>
 +
    </footer>
 +
    <div class="sponsors">
 +
      <div class="container">
 +
        <div class="row">
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.lundbeckfonden.com/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/e/e2/DTU-Denmark_Lundbeckfonden.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.ottomoensted.dk" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/3/35/DTU-Denmark_Ottomoensted.png">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.novonordiskfonden.dk/en" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/5/5c/DTU-Denmark_NovoNordiskFonden_logo.png">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="https://dk.vwr.com/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/e/ea/DTU-Denmark_VWR.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://frisenette.dk" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/c/c8/DTU-Denmark_Frisenette.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.in-vitro.dk/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/7/7c/DTU-Denmark_In_Vitro.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="https://dk.fishersci.com/dk/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/b/b4/DTU-Denmark_Fisher_Scientific.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://us.akg.com/akg-homepage-us.html" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/9/9d/DTU-Denmark_AKG.png">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.macrogen.com/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/1/11/DTU-Denmark_Marcogen.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.snapgene.com/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/5/58/DTU-Denmark_Snapgene.png">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="https://www.neb.com/" target="_blank">
 +
              <img src="https://static.igem.org/mediawiki/2015/e/e0/DTU-Denmark_NEB_logo.png">
 +
            </a>
 +
          </div>
 +
         
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </body>
 +
</html>

Latest revision as of 14:34, 9 November 2015


Overview

Scroll down for more

Project

Introduction

Over the summer we worked with nonribosomal peptide synthetases (NRPSs). We developed a B. subtilis strain capable of oligo-mediated genome engineering and used this strain to alter a NRPS. We also investigated methods of screening for novel products with desired activities.

Background

Nonribosomal peptide synthases (NRPSs) are large multimodular enzymes that synthesize nonribosomal peptides, which are short bioactive peptides with a broad range of functions, including antibiotics, immunosuppressants and anticancer drugs.

Read more

MAGE subtilis

Multiplex automated genome engineering (MAGE) utilises cyclical recombination with short oligonucleotides in order to achieve a high allelic replacement efficiency and can be used to quickly generate cell populations with varying phenotypes. We introduced oligo-mediated genome engineering into Bacillus subtilis and used it to alter a nonribosomal peptide synthetase.

Read more

Tyrocidine

Tyrocidine is a mixture of non-ribosomal peptides. It can only be used topically due to its toxicity. We sought to express the tyrocidine synthase cluster in B. subtilis to make novel derivatives with oligo-mediated recombineering.

Read more

Lab-on-a-disc

Lab-on-a-disc is a potential screening method for our MAGE NRPS products. After screening for antimicrobial activity, prospective MAGE edited NRPS products could be tested for different beneficial properties. One example is a quick screening method for cytotoxicity.

Read more

Intein

When one method fails the Synthesizer team has to innovate! We explored an alternative approach for generating short, cyclized peptides with similar length to tyrocidine by using self-splicing proteins. These short, self-splicing proteins that have no function in the proteins they are a part of, besides catalyzing their own excision after translation, are called inteins. The splicing creates a peptide bond between the two adjacent amino acids next to the inteins and we hypothesized that it could be used to make our cyclic peptides.

Read more

NRP Detection

Separation and identification of our non-ribosomal peptide synthetase (NRPS) products was determined by ultra-high performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry (UHPLC-DAD-QTOFMS). NRPS variants were identified by changes in mass-to-charge ratio and column retention time. 

Read more

Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
P: +45 45 25 25 25
M: dtu-igem-2015@googlegroups.com