Difference between revisions of "Team:HZAU-China/InterLab/Results"

(Created page with "<html> <head> <meta charset="utf-8"> <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:HZAU-China/CSS?action=raw&ctype=text/css" /> <script type="text...")
 
 
(25 intermediate revisions by 5 users not shown)
Line 5: Line 5:
 
<script type="text/javascript" src="https://2015.igem.org/Template:HZAU-China/Javascript/rocketjs?action=raw&ctype=text/javascript"/></script>
 
<script type="text/javascript" src="https://2015.igem.org/Template:HZAU-China/Javascript/rocketjs?action=raw&ctype=text/javascript"/></script>
 
<script type="text/javascript" src="https://2015.igem.org/Template:HZAU-China/Javascript/rocketjquery?action=raw&ctype=text/javascript"/></script>
 
<script type="text/javascript" src="https://2015.igem.org/Template:HZAU-China/Javascript/rocketjquery?action=raw&ctype=text/javascript"/></script>
 +
<script type="text/javascript" src="https://2015.igem.org/Template:HZAU-China/Javascript/floathzaujs?action=raw&ctype=text/javascript"/></script>
 
<style>
 
<style>
 +
.fixbar{
 +
width:100%;
 +
height:53px;
 +
}
  
 
.firstHeading {
 
.firstHeading {
Line 33: Line 38:
 
padding:0px;
 
padding:0px;
 
}
 
}
−
 
 
*{margin:0px;padding:0px;}
 
*{margin:0px;padding:0px;}
 
.box{
 
.box{
 
margin:0 auto;
 
margin:0 auto;
−
background-color:rgba(39,72,98,1.00);
+
background-color:rgba(213,222,217,1.00);
 
}
 
}
 
.topbox{
 
.topbox{
Line 43: Line 47:
 
height:184px;
 
height:184px;
 
}
 
}
−
.middle_topbox h1{
+
.topbox{
−
  border-bottom:hidden;
+
padding: 1em 190px 1em;
−
  font-size:45px;
+
letter-spacing: -1px;
−
  color:#FFF;
+
text-align: center;
−
}
+
padding-top:30px;
−
.middle_topbox h2{
+
−
  color:#FFF;
+
−
  font-size:25px;
+
−
  border-bottom:hidden;
+
 
}
 
}
 
.left_topbox{
 
.left_topbox{
−
float:left;
+
position: absolute;
−
margin:0 40px 0 40px;
+
top:0px;
 +
left:0px;
 +
}
 +
.right_topbox{
 +
position: absolute;
 +
top:0px;
 +
right: 0px;
 
}
 
}
 
.middle_topbox{
 
.middle_topbox{
−
float:left;
+
padding-top:30px;}
−
width:60%;
+
.topbox h1 {
−
padding-top:45px;
+
     
−
text-align:center;
+
    color: #fff;
−
  font-family:Conv_JOKERMAN;
+
font-weight: 600;
 +
font-size: 50px;
 +
line-height: 1;
 +
margin-bottom: 0;
 +
font-family: 'Conv_JOKERMAN',sans-serif;
 +
        border-bottom:hidden;
 
}
 
}
−
.right_topbox{
+
.topbox h1 span {
−
float:left;
+
font-family: 'Conv_JOKERMAN',sans-serif;
−
margin:0 30px 0 30px;
+
display: block;
 +
font-size: 60%;
 +
font-weight: 400;
 +
padding: 0.8em 0 0.5em 0;
 +
color: #c3c8cd;
 +
        border-bottom:hidden;
 
}
 
}
−
.tailbox {
+
.tailbox{
−
clear:both;
+
font-family:Constantia, "Lucida Bright", "DejaVu Serif", Georgia, serif;
−
        font-family:Constantia, "Lucida Bright", "DejaVu Serif", Georgia, serif;
+
color: #fff;
−
font-size:20px;
+
background:rgba(0,0,0,0.75);
−
color:#ffffff;
+
font-size: 1em;
−
padding:20px 40px 70px;
+
padding-bottom:50px;
 
overflow: hidden;
 
overflow: hidden;
 +
width:100%;
 
margin:0 auto;
 
margin:0 auto;
−
height:185px;
+
      background:url(https://static.igem.org/mediawiki/2015/1/18/Team_HZAU_China_hometop_background.png) repeat-y;
−
background:url(https://static.igem.org/mediawiki/2015/1/18/Team_HZAU_China_hometop_background.png);
+
}
−
}
+
 
.left_tailbox{
 
.left_tailbox{
 
float:left;
 
float:left;
 
width:30%;
 
width:30%;
−
margin:20px 0px 0px 180px;
+
margin-left:10%;
 +
        margin-top:30px;
 +
      display:inline;
 
}
 
}
 
.right_tailbox{
 
.right_tailbox{
−
margin:10px 0px 0px 40px;
+
      margin-top:20px;
 +
      display:inline;
 +
margin-left:5%;
 
float:left;
 
float:left;
 
}
 
}
−
.right_tailbox h2{
+
.right_tailbox h1{
−
        border-bottom:hidden;
+
      color:#fff;
−
        color:#fff;
+
−
        font-size:35px;
+
 
}
 
}
 
.contacts_left{
 
.contacts_left{
 
float:left;
 
float:left;
−
width:55%;
+
width:60%;
 
}
 
}
 
.contacts_right{
 
.contacts_right{
−
margin:0px 0px 0px 25px;
+
margin:0px 0px 0px 20px;
 
float:left;
 
float:left;
 
}
 
}
Line 104: Line 122:
 
       list-style-image:none;
 
       list-style-image:none;
 
       list-style-type:disc;
 
       list-style-type:disc;
 +
}
 +
 +
.bar{
 +
      height:53px;
 +
      background:rgba(25,44,60,1.00);
 
}
 
}
 
.bar ul{
 
.bar ul{
 
         padding:0px;
 
         padding:0px;
 
         margin:0px;
 
         margin:0px;
 +
      background:rgba(25,44,60,1.00);
 
}
 
}
 
.bar ul.barname{
 
.bar ul.barname{
Line 115: Line 139:
 
float:right;
 
float:right;
 
padding-right:5%;
 
padding-right:5%;
−
background:rgba(25,44,60,1.00);
 
−
padding-left:214px;
 
 
}
 
}
 
li{
 
li{
Line 160: Line 182:
 
margin:0 auto;
 
margin:0 auto;
 
}
 
}
−
.I li,.T li,.A li,.O li{
+
.P li,.W li,.H li,.I li,.T li,.A li,.O li,.M li{
 
     padding:0px 15px;
 
     padding:0px 15px;
 
}
 
}
−
.I a:link,.T a:link,.A a:link,.O a:link, .I a:visited,.T a:visited,.A a:visited,.O a:visited{
+
.P a:link,.P a:visited,.W a:link,.W a:visited,.H a:link,.H a:visited,.I a:link,.T a:link,.A a:link,.O a:link, .I a:visited,.T a:visited,.A a:visited,.O a:visited,.M a:link,.M a:visited{
 
font-family:Constantia, "Lucida Bright", "DejaVu Serif", Georgia, serif;
 
font-family:Constantia, "Lucida Bright", "DejaVu Serif", Georgia, serif;
 
font-size:20px;
 
font-size:20px;
 
text-decoration:none;
 
text-decoration:none;
 
}
 
}
−
.I a:hover,.I a:active, .T a:hover,.T a:active, .A a:hover,.A a:active, .O a:hover,.O a:active{
+
.P a:hover,.P a:active,.W a:hover,.W a:active,.H a:hover,.H a:active,.I a:hover,.I a:active, .T a:hover,.T a:active, .A a:hover,.A a:active, .O a:hover,.O a:active,.M a:hover,.M a:active{
 
color:#ffffff;
 
color:#ffffff;
 
}
 
}
−
/*.I li:hover,.I li:active, .T li:hover,.T li:active, .A li:hover,.A li:active, .O li:hover,.O li:active{
 
−
 
−
}*/
 
  
 
.maincontent{
 
.maincontent{
 
margin:0 auto;
 
margin:0 auto;
 
width:68%;
 
width:68%;
−
color:rgba(220,220,220,1.00);
+
 
 
line-height:1.5em;
 
line-height:1.5em;
 
font-size:20px;
 
font-size:20px;
 +
font-family: Arial, "Times New Roman", Georgia, Helvetica, Arial, sans-serif;
  
 
}
 
}
Line 200: Line 220:
 
         border-bottom:hidden;
 
         border-bottom:hidden;
 
}
 
}
−
 
+
.maincontent img{
−
 
+
display:block;
 +
margin:0 auto;
 +
}
 +
.maincontent .zhushi{
 +
font-size:18px;
 +
        width:70%;
 +
        clear:both;
 +
padding-left:15%;
 +
}
 +
.maincontent .youyi{
 +
padding-left:5%;
 +
font-style:italic;
 +
}
  
 
#rocket-to-top div {
 
#rocket-to-top div {
Line 248: Line 280:
 
   <div class="topbox">
 
   <div class="topbox">
 
     <div class="left_topbox"><img src="https://static.igem.org/mediawiki/2015/6/6e/Team_HZAU-China_ecoli.png"/></div>
 
     <div class="left_topbox"><img src="https://static.igem.org/mediawiki/2015/6/6e/Team_HZAU-China_ecoli.png"/></div>
−
     <div class="middle_topbox"><h1>Mixed-Reality Cell</h1><h2>Bidirectinal coupling between real and virtual bio-oscillator</h2></div>
+
     <div class="middle_topbox"><h1>Mixed-Reality Cell<span>Bidirectional coupling between real and virtual bio-oscillators</span></h1></div>
 
     <div class="right_topbox"><img src="https://static.igem.org/mediawiki/2015/1/12/Team_HZAU-China_brain.png"></div>
 
     <div class="right_topbox"><img src="https://static.igem.org/mediawiki/2015/1/12/Team_HZAU-China_brain.png"></div>
 
   </div><!--页面头部-->
 
   </div><!--页面头部-->
 
   <div class="middlebox">
 
   <div class="middlebox">
−
    <div class="bar"><!--菜单导航部分-->
+
    <div id="rollchange" class="fixbar">
 +
<div class="bar"><!--菜单导航部分-->
 
       <ul class="barname">
 
       <ul class="barname">
−
         <li><a href="">Home</a></li>
+
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
−
         <li><a href="">Project</a></li>
+
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
−
         <li><a href="">Experiments</a></li>
+
          <ul class="P">
−
         <li><a href="">Modeling</a></li>
+
            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Overview">Overview</a></li>
−
         <li><a href="">HardWare</a></li>
+
            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
−
         <li><a href="">InterLab</a>
+
            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/mrcell">MR.Cell</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Future">Future</a></li>
 +
          </ul>
 +
        </li>
 +
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 +
            <ul class="W">
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Overview">Overview</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Labnote">Labnote</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Protocol">Protocol</a></li>
 +
          </ul>
 +
        </li>
 +
         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 +
            <ul class="M">
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Overview">Overview</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">E-oscillators</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 +
          </ul>
 +
        </li>
 +
         <li><a href="https://2015.igem.org/Team:HZAU-China/Hardware">HardWare</a>
 +
            <ul class="H">
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Overview">Overview</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
 +
          </ul>
 +
        </li>
 +
         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
           <ul class="I">
 
           <ul class="I">
−
             <li><a href="">Protocol</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Overview">Overview</a></li>
−
             <li><a href="">Results</a></li>
+
            <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 +
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
−
           <li><a href="">Achievements</a>
+
           <li><a href="https://2015.igem.org/Team:HZAU-China/Achievements">Achievements</a>
 
           <ul class="A">
 
           <ul class="A">
−
             <li><a href="">Judgement</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Achievements">Judgement</a></li>
−
             <li><a href="">Parts</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Achievements/Parts">Parts</a></li>
 
           </ul>
 
           </ul>
 
           </li>
 
           </li>
−
         <li><a href="">Team</a>
+
         <li><a href="https://2015.igem.org/Team:HZAU-China/Team">Team</a>
 
           <ul class="T">
 
           <ul class="T">
−
             <li><a href="">Members</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Team">Members</a></li>
−
             <li><a href="">Attributions</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Team/Gallary">Gallary</a></li>  
−
            <li><a href="">Gallary</a></li>  
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Team/Attributions">Attributions</a></li>
−
             <li><a href="">Acknowledgement</a></li>
+
−
            <li><a href="">Notebook</a></li>
+
 
           </ul>
 
           </ul>
 
           </li>
 
           </li>
−
         <li><a href="">OutReach</a>
+
         <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">OutReach</a>
 
           <ul class="O">
 
           <ul class="O">
−
             <li><a href="">Human&nbsp;Practice</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">Human&nbsp;Practice</a></li>
−
             <li><a href="">Safety</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 +
            <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
 
       </ul>
 
       </ul>
 
       </div>
 
       </div>
 +
</div>
 +
<script type="text/javascript">$("#rollchange").smartFloat();</script>
 
        
 
        
 
       <div class="maincontent">
 
       <div class="maincontent">
−
       <h1></br></br></br>Overview</h1></br>
+
       <h1></br></br>Results</h1></br>
−
       <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
+
<h3>1. Here are results of per-experiment.</h3>
−
      </br><p>The three genetic devices differ in their promotors :</p>
+
       <p>The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours. </p>
−
      <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
+
</br>
−
      <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
+
<img src="https://static.igem.org/mediawiki/2015/8/82/Team_HZAU-China_interp1.png" width="750px" height="300px">
−
      <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
+
<div class="zhushi"><strong>Fig.1 Growth curve of per-experiment</strong>  As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.</div>
−
      <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
+
</br>
−
       </br></br>
+
<h3>2. Here are results of formal experiment</h3>
 +
<p>Each sample here was given in arbitrary units-fluorescence intensity/OD600.</p>
 +
<p>We used 5ml centrifuge tubes to culture our samples and they were measured by a plate reader after 9 hours’ incubating. Three separate colonies were taken from the same sample.</p>
 +
<br>
 +
<div class="youyi">
 +
<p>The following is our calculation method.</p>
 +
<p>F=F1-F0</p>
 +
<p>O=O1-O0</p>
 +
<p>F/O is the final data.</p>
 +
<p>F1 : Fluorescence intensity of experimental groups(include three devices) .</p>
 +
<p>F0 : Fluorescence intensity of negative control(E.coli with I13504 in pSB1C3).</p>
 +
<p>O1: Optical density of experimental groups (include three devices) .</p>
 +
<p>O0: Optical density of background(LB media).</p>
 +
</div>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2015/7/71/Team_HZAU-China_interp2.png" width="700px" height="800px">
 +
<div class="zhushi"><strong>Fig.2</strong>  A1-A9 are the measured values of Device1 and A1-A3 are one of the measured values of technical repeats. A1-A3, A4-A6 and A7-A9 are biological repeats respectively.B1-B9 and C1-C9 are the measured values of Device 2 and Device 3 respectively. D1-D9 are the measured values of positive control while E1-E9 are the measured values of negative control. F1-F9 are values of background(LB medium).</div>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2015/9/94/Team_HZAU-China_interp3.png" width="720px" height="400px">
 +
<div class="zhushi"><strong>Fig.3</strong>  Device 1: J23101 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 2: J23106 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 3: J23117 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone.</div>
 +
       </br>
 
       </div><!--maincontent结束-->
 
       </div><!--maincontent结束-->
 
        
 
        
Line 306: Line 389:
 
     </div>
 
     </div>
 
     <div class="right_tailbox">
 
     <div class="right_tailbox">
−
       <h2>&nbsp;&nbsp;&nbsp;Contacts</h2>
+
       <h1>&nbsp;&nbsp;&nbsp;Contacts</h1>
 
       <div class="contacts_left">
 
       <div class="contacts_left">
 
         <ul>
 
         <ul>
Line 312: Line 395:
 
             No.1, Shizishan Street, Hongshan District
 
             No.1, Shizishan Street, Hongshan District
 
             </br>
 
             </br>
−
             430070,P.R.China
+
             Wuhan, 430070, Hubei Province, P. R. China
 
             </li>
 
             </li>
 
             <li class="email_inf">Email:hzauigem@gmail.com</li>
 
             <li class="email_inf">Email:hzauigem@gmail.com</li>

Latest revision as of 17:23, 15 November 2015

Mixed-Reality CellBidirectional coupling between real and virtual bio-oscillators



Results


1. Here are results of per-experiment.

The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours.


Fig.1 Growth curve of per-experiment As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.

2. Here are results of formal experiment

Each sample here was given in arbitrary units-fluorescence intensity/OD600.

We used 5ml centrifuge tubes to culture our samples and they were measured by a plate reader after 9 hours’ incubating. Three separate colonies were taken from the same sample.


The following is our calculation method.

F=F1-F0

O=O1-O0

F/O is the final data.

F1 : Fluorescence intensity of experimental groups(include three devices) .

F0 : Fluorescence intensity of negative control(E.coli with I13504 in pSB1C3).

O1: Optical density of experimental groups (include three devices) .

O0: Optical density of background(LB media).


Fig.2 A1-A9 are the measured values of Device1 and A1-A3 are one of the measured values of technical repeats. A1-A3, A4-A6 and A7-A9 are biological repeats respectively.B1-B9 and C1-C9 are the measured values of Device 2 and Device 3 respectively. D1-D9 are the measured values of positive control while E1-E9 are the measured values of negative control. F1-F9 are values of background(LB medium).

Fig.3 Device 1: J23101 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 2: J23106 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 3: J23117 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone.


© 2015 Huazhong Agricultural University iGEM Team. All rights reserved.

   Contacts

  • No.1, Shizishan Street, Hongshan District
    Wuhan, 430070, Hubei Province, P. R. China
  • Wechat : hzauigem
  • QQ Group : 313297095
  • YouTube : hzauigem