Difference between revisions of "Team:HZAU-China/InterLab/Results"

 
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     <div class="left_topbox"><img src="https://static.igem.org/mediawiki/2015/6/6e/Team_HZAU-China_ecoli.png"/></div>
 
     <div class="left_topbox"><img src="https://static.igem.org/mediawiki/2015/6/6e/Team_HZAU-China_ecoli.png"/></div>
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     <div class="middle_topbox"><h1>Mixed-Reality Cell</h1><h2>Bidirectinal coupling between real and virtual bio-oscillator</h2></div>
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     <div class="middle_topbox"><h1>Mixed-Reality Cell<span>Bidirectional coupling between real and virtual bio-oscillators</span></h1></div>
 
     <div class="right_topbox"><img src="https://static.igem.org/mediawiki/2015/1/12/Team_HZAU-China_brain.png"></div>
 
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<div class="bar"><!--菜单导航部分-->
 
       <ul class="barname">
 
       <ul class="barname">
 
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
 
           <ul class="P">
 
           <ul class="P">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Real_Part">Real&nbsp;Part</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/mrcell">MR.Cell</a></li>
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            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Virtual_Part">Virtual&nbsp;Part</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Future">Future</a></li>
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            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Interface_Device">Interface&nbsp;Device</a></li>
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−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Application">Application</a></li>
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           </ul>
 
           </ul>
 
         </li>
 
         </li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 
             <ul class="W">
 
             <ul class="W">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 
             <ul class="M">
 
             <ul class="M">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Overview">Overview</a></li>
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">e-oscillators</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">E-oscillators</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare">HardWare</a>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/Hardware">HardWare</a>
 
             <ul class="H">
 
             <ul class="H">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Overview">Overview</a></li>
−
            <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Background">Background</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
           <ul class="I">
 
           <ul class="I">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
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           </ul>
 
           </ul>
 
           </li>
 
           </li>
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         <li><a href="">OutReach</a>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">OutReach</a>
 
           <ul class="O">
 
           <ul class="O">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Human_Practice">Human&nbsp;Practice</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">Human&nbsp;Practice</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
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       </ul>
 
       </ul>
 
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       <h1></br></br></br>Results</h1></br>
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       <h1></br></br>Results</h1></br>
 
<h3>1. Here are results of per-experiment.</h3>
 
<h3>1. Here are results of per-experiment.</h3>
 
       <p>The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours. </p>
 
       <p>The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours. </p>
 
</br>
 
</br>
 
<img src="https://static.igem.org/mediawiki/2015/8/82/Team_HZAU-China_interp1.png" width="750px" height="300px">
 
<img src="https://static.igem.org/mediawiki/2015/8/82/Team_HZAU-China_interp1.png" width="750px" height="300px">
−
<p class="zhushi">Fig.1 Growth curve of per-experiment</p>
+
<div class="zhushi"><strong>Fig.1 Growth curve of per-experiment</strong> As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.</div>
−
<p>As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.</p>
+
 
</br>
 
</br>
 
<h3>2. Here are results of formal experiment</h3>
 
<h3>2. Here are results of formal experiment</h3>
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<p>F=F1-F0</p>
 
<p>F=F1-F0</p>
 
<p>O=O1-O0</p>
 
<p>O=O1-O0</p>
−
 
+
<p>F/O is the final data.</p>
−
<div>
+
<p>F1 : Fluorescence intensity of experimental groups(include three devices) .</p>
−
      </br></br>
+
<p>F0 : Fluorescence intensity of negative control(E.coli with I13504 in pSB1C3).</p>
 +
<p>O1: Optical density of experimental groups (include three devices) .</p>
 +
<p>O0: Optical density of background(LB media).</p>
 +
</div>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2015/7/71/Team_HZAU-China_interp2.png" width="700px" height="800px">
 +
<div class="zhushi"><strong>Fig.2</strong>  A1-A9 are the measured values of Device1 and A1-A3 are one of the measured values of technical repeats. A1-A3, A4-A6 and A7-A9 are biological repeats respectively.B1-B9 and C1-C9 are the measured values of Device 2 and Device 3 respectively. D1-D9 are the measured values of positive control while E1-E9 are the measured values of negative control. F1-F9 are values of background(LB medium).</div>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2015/9/94/Team_HZAU-China_interp3.png" width="720px" height="400px">
 +
<div class="zhushi"><strong>Fig.3</strong>  Device 1: J23101 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 2: J23106 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 3: J23117 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone.</div>
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       <h2>&nbsp;&nbsp;&nbsp;Contacts</h2>
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       <h1>&nbsp;&nbsp;&nbsp;Contacts</h1>
 
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             No.1, Shizishan Street, Hongshan District
 
             No.1, Shizishan Street, Hongshan District
 
             </br>
 
             </br>
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             430070,P.R.China
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             Wuhan, 430070, Hubei Province, P. R. China
 
             </li>
 
             </li>
 
             <li class="email_inf">Email:hzauigem@gmail.com</li>
 
             <li class="email_inf">Email:hzauigem@gmail.com</li>

Latest revision as of 17:23, 15 November 2015

Mixed-Reality CellBidirectional coupling between real and virtual bio-oscillators



Results


1. Here are results of per-experiment.

The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours.


Fig.1 Growth curve of per-experiment As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.

2. Here are results of formal experiment

Each sample here was given in arbitrary units-fluorescence intensity/OD600.

We used 5ml centrifuge tubes to culture our samples and they were measured by a plate reader after 9 hours’ incubating. Three separate colonies were taken from the same sample.


The following is our calculation method.

F=F1-F0

O=O1-O0

F/O is the final data.

F1 : Fluorescence intensity of experimental groups(include three devices) .

F0 : Fluorescence intensity of negative control(E.coli with I13504 in pSB1C3).

O1: Optical density of experimental groups (include three devices) .

O0: Optical density of background(LB media).


Fig.2 A1-A9 are the measured values of Device1 and A1-A3 are one of the measured values of technical repeats. A1-A3, A4-A6 and A7-A9 are biological repeats respectively.B1-B9 and C1-C9 are the measured values of Device 2 and Device 3 respectively. D1-D9 are the measured values of positive control while E1-E9 are the measured values of negative control. F1-F9 are values of background(LB medium).

Fig.3 Device 1: J23101 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 2: J23106 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone. Device 3: J23117 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone.


© 2015 Huazhong Agricultural University iGEM Team. All rights reserved.

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