Difference between revisions of "Team:HZAU-China/Team/Attributions"

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     <div class="middle_topbox"><h1>Mixed-Reality Cell<span>Bidirectinal coupling between real and virtual bio-oscillator</span></h1></div>
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     <div class="middle_topbox"><h1>Mixed-Reality Cell<span>Bidirectional coupling between real and virtual bio-oscillators</span></h1></div>
 
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       <ul class="barname">
 
       <ul class="barname">
 
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
 
           <ul class="P">
 
           <ul class="P">
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Overview</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Real_Part">Real&nbsp;Part</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/mrcell">MR.Cell</a></li>
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            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Virtual_Part">Virtual&nbsp;Part</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Future">Future</a></li>
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            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Interface_Device">Interface&nbsp;Device</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Application">Application</a></li>
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           </ul>
 
           </ul>
 
         </li>
 
         </li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 
             <ul class="W">
 
             <ul class="W">
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             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">Overview</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 
             <ul class="M">
 
             <ul class="M">
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Overview</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Overview">Overview</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">e-oscillators</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">E-oscillators</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 
           </ul>
 
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         <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare">HardWare</a>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/Hardware">HardWare</a>
 
             <ul class="H">
 
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             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare">Overview</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Overview">Overview</a></li>
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            <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Background">Background</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
           <ul class="I">
 
           <ul class="I">
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             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">Overview</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
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           </li>
 
           </li>
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         <li><a href="">OutReach</a>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">OutReach</a>
 
           <ul class="O">
 
           <ul class="O">
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             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Human_Practice">Human&nbsp;Practice</a></li>
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             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">Human&nbsp;Practice</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
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       <h1></br></br></br>Overview</h1></br>
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       <h1></br></br>Attributions</h1></br>
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      <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
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      <p>Firstly we would like to thanks ourselves for insisting on such a long time to struggle with this competition.</p></br>
−
      </br><p>The three genetic devices differ in their promotors :</p>
+
<p><strong>There are our attributions as follows :</strong></p>
−
      <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
+
<p><strong>WetLab:</strong></p>
−
      <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Construction of quorum sensing oscillator: Yan Kji, Yang Junqi, Wu Yibo, Guo Xiaoxue, Yang main. </p>
−
      <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Construction dual feedback oscillator: Luo Xudong, Chen Yu, Wu Yibo</p>
−
      <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Hybrid promote test: Yan Keji, Yang Junqi</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Light control system test: Yan Keji</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Interlab：Pan Chu, Jun Li</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Construction of aptamer: Zhu Qi</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Aptamer test: Zhu Qi</p>
 +
 
 +
<p><strong>Model and data analysis :</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Model construction:Performed by Li Zeyao, Hua Kangjian, Liu Kai and Wang Shuangqi.</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Compling:Achieved by Li Zeyao, Hua Kangjian.</p>
 +
 
 +
<p><strong>Hardware Construction:</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Design and program of device: Zhang Qian, Hua Kangjian</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Design and work on microfluidic chip: Chu Pan</p>
 +
 
 +
<p><strong>InterLab:</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Chu Pan, Li Jun, helped by Yan Keji</p>
 +
<p><strong>Art design :</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Created by Xiao Yi, helped by Hua Kangjian.</p>
 +
<p><strong>Wiki :</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Displayed by Wang Shuangqi, Hua Kangjian and Written by all of us!</p>
 +
<p><strong>Safety:</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Finished by Wu Yibo and Yang Junqi, helped by Yang Mian.</p>
 +
<p><strong>HP&Policy :</strong></p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Organized by Li Shangjin, participated by all of us!</p>
 +
</br>
 +
<p>Conference of three university of Central China</p>
 +
<p>Attendees :All of us.</p>
 +
</br>
 +
<p>2015 International Genetically Engineered Machine Conference:</p>
 +
<p>Attendees : Luo Xudong, Li Zeyao, Wu Yibo, Yang Junqi, Zhu Qi, Zhang Qian.</p>
 +
</br>
 +
<p>CCiC meetup(Conference of China iGEMer Committee):</p>
 +
<p>Attendees : Yan Keji, Hua Kangjian, Li Shangjin, Chen Yu, Chu Pan, Li Jun, Wang Shuangqi.</p>
 +
</br>
 +
 
 +
<h3>Acknowledgement</h3>
 +
<p>Besides, we want to appreciate the following people, institutions, laboratories and organizations for the help in this year :</p>
 +
</br>
 +
<p> <strong>Lab and Fund supports :</strong></p>
 +
<p>College of Life science and Technology of Huazhong Agricultural University</p>
 +
<p>College of Informatics of Huazhong Agricultural University</p>
 +
<p>Teaching experimental center of College of Life science and Technology</p>
 +
<p>Innovation Lab of College of Life science and Technology</p>
 +
<p>College of Sciences of Huazhong Agricultural University</p>
 +
<p>National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University</p>
 +
<p>National Key Laboratory of Agro-microbiology of Huazhong Agricultural University</p>
 +
<p>National Natural Science Foundation of China</p>
 +
<p>Office of Academic Affairs of Huazhong Agricultural University</p>
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<p>College of Life science and Technology of Huazhong Agricultural University</p>
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<p>ZhangZhiDong Class of Huazhong Agricultural University</p>
 +
<p>College of fisheries of Huazhong Agricultural University</p>
 +
</br>
 +
<p>Special thanks to Jin Anjiang and Huang Wei, who went to great lengths to help us smooth over many administrative troubles. We should also thank professors Ma Binguang, Ni Fuchuang and Gao Yijun, who taught us with great patience how to use singlechip to program for our project and friendly give us suggestions. And besides, we should also thank professors Kang Qinshu, and Gao jun who kindly guided us through the microfluidic and the construction of oscillators.</p>
 +
</br>
 +
<p><strong>External support</strong></p>
 +
<p>Thanks for the iGEM foundation for providing us with most of our materials, and we should also thanks the following people for giving us additional supports.</p>
 +
<p>Thanks professor Jaffrey for allowing us to use and submit the aptamar.</p>
 +
<p>Thanks Ku Jing, graduate student of HZAU and Chen Xuanhong, graduate student of ZJU for some help of Interface software.</p>
 +
<p>Thanks the clerk of TsingKe company named Zhang Junjie who helped us with the synthesis of the aptamer.</p>
 +
<p>Meanwhile, we should thank WHU-China, who generously offered us with some parts and device that we didn't have. And thanks to ZJU-China, who shared us a basic program as a reference.</p>
 +
 
 +
</br>
 +
<p><strong>Project support and advice :</strong></p>
 +
<p>We appreciate Tian Liu(the captain of 2014 iGEM) most for helping us in all aspects of our project constantly. No matter the wetLab, modeling, activites or other parts. He has been giving us advice and ideas.</p>
 +
<p>We thanks to Han Yichao(a 2014 iGEMer) for giving us some suggestions about our modeling part. From him, we have saved a lot of time.</p>
 +
</br>
 +
<p>Besides the people mentioned above, we should also thank the following professors and upperclassmen, who offered many suggestions to our project:</p>
 +
</br>
 +
<p>Ma Binguang, Peng Donghai, Zhang Hongyu, Zhen Yonglian ,Zhen Shixue, Cao Gang, Wang Gejiao, He Jin, Song Ming and Gao Jun.</p>
 +
</br>
 +
<p><strong>Special</strong></p>
 +
<p>We must thank our two instructors, professor Zhang Hongyu and professor Ma Binguang, especially professor Ma, who devoted a lot of time efforts into us and has been with us throughout the whole competition. And we must thank the members of last year's HZAU-China, without their efforts and achievements, we wouldn't have got sufficient support this year, and they are: Tian Liu, Lu Huifang, Han Yichao, Zhang Peijing, Zhen Yihui, Li Fuyao</p>
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      <h1>&nbsp;&nbsp;&nbsp;Contacts</h1>
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      <h1>&nbsp;&nbsp;&nbsp;Contact Information</h1>
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            No.1, Shizishan Street, Hongshan District
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                      No.1, Shizishan Street, Hongshan District,
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            430070,P.R.China
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                      Wuhan, 430070, Hubei Province, P. R. China
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            <li class="email_inf">Email:hzauigem@gmail.com</li>
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                      <li class="email_inf">Email: hzauigem@gmail.com</li>
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Latest revision as of 17:28, 15 November 2015

Mixed-Reality CellBidirectional coupling between real and virtual bio-oscillators



Attributions


Firstly we would like to thanks ourselves for insisting on such a long time to struggle with this competition.


There are our attributions as follows :

WetLab:

        Construction of quorum sensing oscillator: Yan Kji, Yang Junqi, Wu Yibo, Guo Xiaoxue, Yang main.

        Construction dual feedback oscillator: Luo Xudong, Chen Yu, Wu Yibo

        Hybrid promote test: Yan Keji, Yang Junqi

        Light control system test: Yan Keji

        Interlab：Pan Chu, Jun Li

        Construction of aptamer: Zhu Qi

        Aptamer test: Zhu Qi

Model and data analysis :

        Model construction:Performed by Li Zeyao, Hua Kangjian, Liu Kai and Wang Shuangqi.

        Compling:Achieved by Li Zeyao, Hua Kangjian.

Hardware Construction:

        Design and program of device: Zhang Qian, Hua Kangjian

        Design and work on microfluidic chip: Chu Pan

InterLab:

        Chu Pan, Li Jun, helped by Yan Keji

Art design :

        Created by Xiao Yi, helped by Hua Kangjian.

Wiki :

        Displayed by Wang Shuangqi, Hua Kangjian and Written by all of us!

Safety:

        Finished by Wu Yibo and Yang Junqi, helped by Yang Mian.

HP&Policy :

        Organized by Li Shangjin, participated by all of us!


Conference of three university of Central China

Attendees :All of us.


2015 International Genetically Engineered Machine Conference:

Attendees : Luo Xudong, Li Zeyao, Wu Yibo, Yang Junqi, Zhu Qi, Zhang Qian.


CCiC meetup(Conference of China iGEMer Committee):

Attendees : Yan Keji, Hua Kangjian, Li Shangjin, Chen Yu, Chu Pan, Li Jun, Wang Shuangqi.


Acknowledgement

Besides, we want to appreciate the following people, institutions, laboratories and organizations for the help in this year :


Lab and Fund supports :

College of Life science and Technology of Huazhong Agricultural University

College of Informatics of Huazhong Agricultural University

Teaching experimental center of College of Life science and Technology

Innovation Lab of College of Life science and Technology

College of Sciences of Huazhong Agricultural University

National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University

National Key Laboratory of Agro-microbiology of Huazhong Agricultural University

National Natural Science Foundation of China

Office of Academic Affairs of Huazhong Agricultural University

College of Life science and Technology of Huazhong Agricultural University

ZhangZhiDong Class of Huazhong Agricultural University

College of fisheries of Huazhong Agricultural University


Special thanks to Jin Anjiang and Huang Wei, who went to great lengths to help us smooth over many administrative troubles. We should also thank professors Ma Binguang, Ni Fuchuang and Gao Yijun, who taught us with great patience how to use singlechip to program for our project and friendly give us suggestions. And besides, we should also thank professors Kang Qinshu, and Gao jun who kindly guided us through the microfluidic and the construction of oscillators.


External support

Thanks for the iGEM foundation for providing us with most of our materials, and we should also thanks the following people for giving us additional supports.

Thanks professor Jaffrey for allowing us to use and submit the aptamar.

Thanks Ku Jing, graduate student of HZAU and Chen Xuanhong, graduate student of ZJU for some help of Interface software.

Thanks the clerk of TsingKe company named Zhang Junjie who helped us with the synthesis of the aptamer.

Meanwhile, we should thank WHU-China, who generously offered us with some parts and device that we didn't have. And thanks to ZJU-China, who shared us a basic program as a reference.


Project support and advice :

We appreciate Tian Liu(the captain of 2014 iGEM) most for helping us in all aspects of our project constantly. No matter the wetLab, modeling, activites or other parts. He has been giving us advice and ideas.

We thanks to Han Yichao(a 2014 iGEMer) for giving us some suggestions about our modeling part. From him, we have saved a lot of time.


Besides the people mentioned above, we should also thank the following professors and upperclassmen, who offered many suggestions to our project:


Ma Binguang, Peng Donghai, Zhang Hongyu, Zhen Yonglian ,Zhen Shixue, Cao Gang, Wang Gejiao, He Jin, Song Ming and Gao Jun.


Special

We must thank our two instructors, professor Zhang Hongyu and professor Ma Binguang, especially professor Ma, who devoted a lot of time efforts into us and has been with us throughout the whole competition. And we must thank the members of last year's HZAU-China, without their efforts and achievements, we wouldn't have got sufficient support this year, and they are: Tian Liu, Lu Huifang, Han Yichao, Zhang Peijing, Zhen Yihui, Li Fuyao




© 2015 Huazhong Agricultural University iGEM Team. All rights reserved.

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