Difference between revisions of "Team:HZAU-China/OutReach/Safety"

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   <div class="topbox">
 
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     <div class="left_topbox"><img src="https://static.igem.org/mediawiki/2015/6/6e/Team_HZAU-China_ecoli.png"/></div>
 
     <div class="left_topbox"><img src="https://static.igem.org/mediawiki/2015/6/6e/Team_HZAU-China_ecoli.png"/></div>
−
     <div class="middle_topbox"><h1>Mixed-Reality Cell<span>Bidirectinal coupling between real and virtual bio-oscillator</span></h1></div>
+
     <div class="middle_topbox"><h1>Mixed-Reality Cell<span>Bidirectional coupling between real and virtual bio-oscillators</span></h1></div>
 
     <div class="right_topbox"><img src="https://static.igem.org/mediawiki/2015/1/12/Team_HZAU-China_brain.png"></div>
 
     <div class="right_topbox"><img src="https://static.igem.org/mediawiki/2015/1/12/Team_HZAU-China_brain.png"></div>
 
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   <div class="middlebox">
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<div class="bar"><!--菜单导航部分-->
 
       <ul class="barname">
 
       <ul class="barname">
 
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China">Home</a></li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Project</a>
 
           <ul class="P">
 
           <ul class="P">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Background">Background</a></li>
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Real_Part">Real&nbsp;Part</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/mrcell">MR.Cell</a></li>
−
            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Virtual_Part">Virtual&nbsp;Part</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Future">Future</a></li>
−
            <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Interface_Device">Interface&nbsp;Device</a></li>
+
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Project/Application">Application</a></li>
+
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">WetLab</a>
 
             <ul class="W">
 
             <ul class="W">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Design">Design</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/WetLab/Characterization">Characterization</a></li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Modeling</a>
 
             <ul class="M">
 
             <ul class="M">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Overview">Overview</a></li>
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">e-oscillators</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/e-oscillators">E-oscillators</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Regulation_to_oscillator">Regulation&nbsp;to&nbsp;oscillator</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/Modeling/Coupling">Coupling</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
−
         <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare">HardWare</a>
+
         <li><a href="https://2015.igem.org/Team:HZAU-China/Hardware">HardWare</a>
 
             <ul class="H">
 
             <ul class="H">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Overview">Overview</a></li>
−
            <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Background">Background</a></li>
+
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Interface_Device">Interface&nbsp;Device</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/HardWare/Mixed-Reality_of_physics">Mixed&nbsp;Reality&nbsp;of&nbsp;physics</a></li>
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         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
         <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 
           <ul class="I">
 
           <ul class="I">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab">Overview</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Overview">Overview</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Protocol">Protocol</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/InterLab/Results">Results</a></li>
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           </ul>
 
           </ul>
 
           </li>
 
           </li>
−
         <li><a href="">OutReach</a>
+
         <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">OutReach</a>
 
           <ul class="O">
 
           <ul class="O">
−
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Human_Practice">Human&nbsp;Practice</a></li>
+
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach">Human&nbsp;Practice</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Safety">Safety</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
 
             <li><a href="https://2015.igem.org/Team:HZAU-China/OutReach/Collaboration">Collaboration</a></li>
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       </ul>
 
       </ul>
 
       </div>
 
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       <div class="maincontent">
 
       <div class="maincontent">
−
       <h1></br></br></br>Overview</h1></br>
+
       <h1></br></br>Safety</h1></br>
−
      <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
+
      <p><strong>We have finished all of the safety forms:</strong></p>
−
      </br><p>The three genetic devices differ in their promotors :</p>
+
<p>Check in form</p>
−
      <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
+
<p>About our lab</p>
−
      <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
+
<p>About our project</p>
−
      <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
+
<p><a href="https://2015.igem.org/File:HZAU-China_Safety2015_Spreadsheet.xls">Final safety form</a></p>
−
      <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
+
</br>
 +
<h3>General safety matters:</h3>
 +
<p>In our lab there is a safety training for everyone which covered following aspects:</p>
 +
<p> 1. Personal prospective wear: lab coat and gloves must be worn all the time during work</p>
 +
<p> 2. Chemical safety: we have printed “Dos and don’ts when using hazardous chemicals” and posted them on marked place.</p>
 +
<p> 3. GMO safety: no biology materials are allowed to bring out of our lab and biology waste must be put to bacterial waste.</p>
 +
</br>
 +
<div class="juzhong">
 +
<img src="https://static.igem.org/mediawiki/2015/5/50/Hzau-%E5%AE%9E%E9%AA%8C%E5%AE%A4_%283%29.jpg" width="360px" height="480px">
 +
<img src="https://static.igem.org/mediawiki/2015/a/af/Hzau-%E5%AE%9E%E9%AA%8C%E6%9C%8D.JPG" width="360px" height="480px">
 +
</div>
 +
</br></br>
 +
<p> 4. Studying biosafety: we have also listed some websites of biosafety for our teammates to study:</p>
 +
</br>
 +
<p>http://swx.hzau.edu.cn/</p>
 +
<p>http://bw.hzau.edu.cn/zc_detail.asp?id=196</p>
 +
</br>
 +
<p>Govern biosafety in research laboratories:</p>
 +
<p>chinahttp://www.stee.agri.gov.cn/biosafety/</p>
 +
<p>http://english.biosafety.gov.cn/</p>
 +
</br>
 +
<p>Biosafety guidelines of our institution:</p>
 +
<p>http://swx.hzau.edu.cn/</p>
 +
<p>http://bw.hzau.edu.cn/zc_detail.asp?id=196</p>
 +
</br>
 +
<p>Regulations that govern biosafety in research laboratories:</p>
 +
<p>http://www.stee.agri.gov.cn/biosafety/</p>
 +
<p>http://english.biosafety.gov.cn/</p>
 +
<h3>Our lab</h3>
 +
<p>Our lab locates in the building of college of life science and technology in Huazhong Agricultural. </p>
 +
</br>
 +
<div class="juzhong">
 +
<img src="https://static.igem.org/mediawiki/2015/f/f2/Hzau-%E5%AE%9E%E9%AA%8C%E5%AE%A4_%284%29.jpg" width="400px" height="480px">
 +
<img src="https://static.igem.org/mediawiki/2015/5/50/Hzau-%E5%AE%9E%E9%AA%8C%E5%AE%A4_%283%29.jpg" width="320px" height="480px">
 +
</div>
 +
</br>
 +
</br></br>
 +
<p>There are two access control system to prevent unauthorized persons form entering, you have to show your student ID card to get into our lab.</p>
 +
</br>
 +
<div class="juzhong">
 +
<img src="https://static.igem.org/mediawiki/2015/2/29/Hzau-safety_inside_%282%29.JPG" width="360px" height="270px">
 +
<img src="https://static.igem.org/mediawiki/2015/c/c1/Hzau-safety_outside.JPG" width="360px" height="270px">
 +
</div>
 +
</br>
 +
</br>
 +
<h3>Our project</h3>
 +
<p>The organisms we used in our project is a kind of non-toxic E.coli(k-12 derivatives) we used sequences that are already available as Biobricks and we didn’t create new protein coding sequences. We use GoldViewTM as Nucleic Acid Gel Stain, lab gloves must be worn when we use it.</p>
 
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             No.1, Shizishan Street, Hongshan District
 
             No.1, Shizishan Street, Hongshan District
 
             </br>
 
             </br>
−
            430070,P.R.China
+
            Wuhan, 430070, Hubei Province, P. R. China
 
             </li>
 
             </li>
 
             <li class="email_inf">Email:hzauigem@gmail.com</li>
 
             <li class="email_inf">Email:hzauigem@gmail.com</li>

Latest revision as of 17:32, 15 November 2015

Mixed-Reality CellBidirectional coupling between real and virtual bio-oscillators



Safety


We have finished all of the safety forms:

Check in form

About our lab

About our project

Final safety form


General safety matters:

In our lab there is a safety training for everyone which covered following aspects:

1. Personal prospective wear: lab coat and gloves must be worn all the time during work

2. Chemical safety: we have printed “Dos and don’ts when using hazardous chemicals” and posted them on marked place.

3. GMO safety: no biology materials are allowed to bring out of our lab and biology waste must be put to bacterial waste.




4. Studying biosafety: we have also listed some websites of biosafety for our teammates to study:


http://swx.hzau.edu.cn/

http://bw.hzau.edu.cn/zc_detail.asp?id=196


Govern biosafety in research laboratories:

chinahttp://www.stee.agri.gov.cn/biosafety/

http://english.biosafety.gov.cn/


Biosafety guidelines of our institution:

http://swx.hzau.edu.cn/

http://bw.hzau.edu.cn/zc_detail.asp?id=196


Regulations that govern biosafety in research laboratories:

http://www.stee.agri.gov.cn/biosafety/

http://english.biosafety.gov.cn/

Our lab

Our lab locates in the building of college of life science and technology in Huazhong Agricultural.





There are two access control system to prevent unauthorized persons form entering, you have to show your student ID card to get into our lab.




Our project

The organisms we used in our project is a kind of non-toxic E.coli(k-12 derivatives) we used sequences that are already available as Biobricks and we didn’t create new protein coding sequences. We use GoldViewTM as Nucleic Acid Gel Stain, lab gloves must be worn when we use it.




© 2015 Huazhong Agricultural University iGEM Team. All rights reserved.

   Contacts

  • No.1, Shizishan Street, Hongshan District
    Wuhan, 430070, Hubei Province, P. R. China
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