Difference between revisions of "Team:Macquarie Australia/ProjectOverview"

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<h2>Project Overview</h2>
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<p>Our project has its focus on photosynthesis - the natural process where plants and algae convert sunlight into useable energy. By developing artificial photosynthesis in a biological system we can better harvest the unlimited supply of solar energy. The long-term goal is to engineer bacteria that can produce hydrogen gas on an industrial scale.</p>
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<h2>Project Overview</h2>
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<p>This year the aim of our team is to engineer bacteria to manufacture chlorophyll, the primary molecule of photosynthesis. Chlorophyll harvests light and is involved in the excitation transfer of energy. Chlorophyll-<i>a</i> can be synthesised via a pathway from the protoporphyrin-IX molecule. By placing 13 genes into 4 biobrick vectors we can recreate the pathway in <i>Escherichia coli</i>.</p>
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<p>There are three main components in our project this year:</p>
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<li><b>Chlorophyll Biosynthesis Pathway</b></li>
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<li>By expressing 4 operons the protoporphyrin-IX molecule can be transformed into chlorophyll-<i>a</i>.</li>
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<li>Protoporphyrin-IX is present within <i>E. coli</i> and is typically used to make a different tetrapyrrole: heme.</li>
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<li><b>Photosystem II</b></li>
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<li>Through the expression of 5 operons we aim to induce <i>E. coli</i> to build the Photosystem II protein complex which contains the Oxygen Evolving Centre of photosynthesis.</li>
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<li><b>Hydrogenase</b></li>
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<li>A hydrogenase enzyme can take two protons and two electrons to build H<sub>2</sub> (molecular hydrogen).</li>
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Latest revision as of 12:08, 19 November 2015

Project Overview
Project page
Link to Project Description page
Link to Experiments & Protocols page
Link to Results page
Link to Notebook page
Link to Safety page

Project Overview

There are three main components in our project this year:

  • Chlorophyll Biosynthesis Pathway
    • By expressing 4 operons the protoporphyrin-IX molecule can be transformed into chlorophyll-a.
    • Protoporphyrin-IX is present within E. coli and is typically used to make a different tetrapyrrole: heme.
  • Photosystem II
    • Through the expression of 5 operons we aim to induce E. coli to build the Photosystem II protein complex which contains the Oxygen Evolving Centre of photosynthesis.
  • Hydrogenase
    • A hydrogenase enzyme can take two protons and two electrons to build H2 (molecular hydrogen).

Chlorophyll Biosynthesis Pathway diagram

Photosystem II diagram
Hydrogenase diagram