Difference between revisions of "Team:Macquarie Australia/Parts"

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<title>Parts</title>
 
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<figure class="specialInline"><a href="https://2015.igem.org/Team:Macquarie_Australia/PartsOverview"><img src="https://static.igem.org/mediawiki/2015/9/9a/MqAust_3Parts_v05a-150dpi.png" width="220px" alt="Link to Parts page"></a></figure>
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<figure class="specialInline"><img src="https://static.igem.org/mediawiki/2015/b/be/MqAust_BubbleParts_1TeamParts.png" width="110px" alt="Team Parts page"></figure>
 
<figure class="specialInline"><img src="https://static.igem.org/mediawiki/2015/b/be/MqAust_BubbleParts_1TeamParts.png" width="110px" alt="Team Parts page"></figure>
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<figure class="specialInline"><a href="https://2015.igem.org/Team:Macquarie_Australia/Basic_Part"><img src="https://static.igem.org/mediawiki/2015/f/f8/MqAust_BubbleParts_2Basic.png" width="110px" alt="Link to Basic Parts page"></a></figure>
 
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<figure class="specialInline"><a href="https://2015.igem.org/Team:Macquarie_Australia/Composite_Part"><img src="https://static.igem.org/mediawiki/2015/a/a3/MqAust_BubbleParts_3Composite.png" width="110px" alt="Link to Composite Parts page"></a></figure>
 
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<figure class="specialInline"><a href="https://2015.igem.org/Team:Macquarie_Australia/Part_Collection"><img src="https://static.igem.org/mediawiki/2015/4/4f/MqAust_BubbleParts_4Collection.png" width="110px" alt="Link to Part Collection page"></a></figure>
 
 
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<h2> Part Documentation</h2>
 
<h2> Part Documentation</h2>
  
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<h4>Part Table </h4>
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<p>We have designed and constructed 10 parts this year, 5 of which have been submitted to iGem HQ.</p>
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<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1640018">BBa_K1640018</a>, aka <b>ChlM</b>, is a part integral to our synthetic chlorophyll biosynthesis pathway, which we have redesigned from earlier submissions, and have functionally characterised. This part has been submitted, and we are claiming a silver medal for this part.</p>
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<th class="regularCol10" style="text-align:center">Part number / name</th>
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<th class="regularCol40" style="text-align:center">Natural function of part</th>
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<p>ChlM is incorporated into the Chlorophyll a biosynthesis pathway in operon 3 as illustrated in the following diagram.</p>
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<th class="regularCol20" style="text-align:center">How will we use it?</th>
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<br>
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<th class="regularCol20" style="text-align:center">Notes</th>
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<center><img src="https://static.igem.org/mediawiki/2015/9/98/MqAust_ProjectDesign_CBP.png" width=600px></center><br>
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<p>Parts <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1640008">BBa_K1640008</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1640020">BBa_K1640020</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1640006">BBa_K1640006</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1640012">BBa_K1640012</a> (respectively <b>psbMZH</b>, <b>psbA</b>, <b>psbELJ</b>, and <b>psbQR</b>) are 4 of the 10 biobricks we have designed for synthetic production of <i>Chlamydomonas reinhardtii</i>'s photosystem II complex. These 4 parts have been submitted to iGem HQ and we are claiming our bronze medal for these new submissions.</p>
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<td><a href="http://parts.igem.org/Part:BBa_K1640000" target="_blank">BBa_K1640000</a></td>
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<p>These photosystem II biobricks are incorporated into the photosystem II biosynthesis pathway as illustrated in the following diagram.</p>
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<td>ChlI1 and ChlD genes transcibe proteins that self-assemble to form a functional ChlI1:ChlD complex. The protein transcribed by the gene ChlH inserts magnesium into protoporphyrin IX. ChlI1 and ChlD genes transcibe proteins that self-assemble to form a functional ChlI1:ChlD complex. The protein transcribed by the gene ChlH inserts magnesium into protoporphyrin IX.</td>
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<br><center><img src="https://static.igem.org/mediawiki/2015/1/14/MqAust_ProjectDesign_PSB.png" width="600px" alt="PSII diagram"></center><br>
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<td>We will use this part to form part of the chlorophyll biosynthesis pathway.</td>
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<td>Composed of Lac promoter (<a href="http://parts.igem.org/Part:BBa_R0010" target="_blank">BBa_R0010</a>), ChlI1 (<a href="http://parts.igem.org/Part:BBa_K1080000" target="_blank">BBa_K1080000</a>), ChlD (<a href="http://parts.igem.org/Part:BBa_K1080002" target="_blank">BBa_K1080002</a>), Gun 4 (<a href="http://parts.igem.org/Part:BBa_K1080003" target="_blank">BBa_K1080003</a>) and ChlH (<a href="http://parts.igem.org/Part:BBa_K1080001" target="_blank">BBa_K1080001</a>) parts.</td>
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<h4>Part Table</h4>
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</html><groupparts>iGEM015 Macquarie_Australia</groupparts><html>
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<td><a href="http://parts.igem.org/Part:BBa_K1640001" target="_blank">BBa_K1640001</a></td>
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<td>CTH1, Plastocyanin, and YCF54 are involved in the oxidative cyclase pathway. ChlM methylates Mg-protoporphyrin IX, facilitating the highly-regulated catalysis of Mg-chelatase. CTH1 catalyses the conversion of Mg-protoporphyrin IX monomethyl into divinyl protochlorophyllide, interacting with YCF54 and Plastocyanin. ChlH inserts magnesium into protoporphyrin IX.</td>
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<td>We will use this part to form part of the chlorophyll biosynthesis pathway.</td>
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<td>Composed of the lac promoter (<a href="http://parts.igem.org/Part:BBa_R0010" target="_blank">BBa_R0010</a>), CTH1 (<a href="http://parts.igem.org/Part:BBa_K1080020" target="_blank">BBa_K1080020</a>), YCF54 (<a href="http://parts.igem.org/Part:BBa_K1080023" target="_blank">BBa_K1080023</a>), Plasto (<a href="http://parts.igem.org/Part:BBa_K1080014" target="_blank">BBa_K1080014</a>), and ChlM (<a href="http://parts.igem.org/Part:BBa_K1326001" target="_blank">BBa_K1326001</a>).</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640002" target="_blank">BBa_K1640002</a></td>
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<td>DVR1 reduces divinyl protochlorophyllide, POR converts protochlorophyllide to chlorophyllide, ChlG adds the geranylgeranyl pyrophosphate chain to the chlorophyllide molecule, and ChlP reduces the double bonds on GGPP. The final product is chlorophyll-<i>a</i>.</td>
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<td>We will use this part to form part of the chlorophyll biosynthesis pathway.</td>
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<td>Composed of the lac promoter (<a href="http://parts.igem.org/Part:BBa_R0010" target="_blank">BBa_R0010</a>), POR (<a href="http://parts.igem.org/Part:BBa_K1080015" target="_blank">BBa_K1080015</a>), DVR1 (<a href="http://parts.igem.org/Part:BBa_K1080012" target="_blank">BBa_K1080012</a>), ChlP (<a href="http://parts.igem.org/Part:BBa_K1080008" target="_blank">BBa_K1080008</a>), and ChlG (<a href="http://parts.igem.org/Part:BBa_K1080009" target="_blank">BBa_K1080009</a>).</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640003" target="_blank">BBa_K1640003</a></td>
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<td>The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction centre core.</td>
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<td>We will use this part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640004" target="_blank">BBa_K1640004</a></td>
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<td>The psbC gene that comprises this part codes a subunit that makes up the Photosystem II protein P680. The P680 complex is photosystem II primary electron donor.</td>
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<td>We will use this part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640020" target="_blank">BBa_K1640020</a></td>
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<td>The psbA gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction centre core.</td>
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<td>We will use this part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640006" target="_blank">BBa_K1640006</a></td>
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<td>This part is composed of psbE, psbL and psbJ genes. The protein encoded by psbE binds heme. The psbJ protein subunit is required for correct Photosystem II assembly. The psbJ protein is required for the stability of the Mn cluster in the Oxygen Evolution Complex.</td>
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<td>We will use this part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640007" target="_blank">BBa_K1640007</a></td>
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<td>This part is composed of the psbT and psbB genes. The psbT protein's function is not well known however it appears to be involved in the dimerization of Photosystem II and is essential for photosynthetic activity.</td>
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<td>We will use this part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640008" target="_blank">BBa_K1640008</a></td>
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<td>This part is composed of the psbM, psbZ, and psbH genes. The psbM protein subunit is positioned at the monomer-monomer interface. The psbZ protein controls the interaction of Photosystem II cores with the light-harvesting antenna. The psbH protein is required for stability and assembly of the photosystem II complex.</td>
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<td>We will use this part to form a composite and test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640009" target="_blank">BBa_K1640009</a></td>
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<td>This part is composed of psbW and psbK genes. The psbW protein stabilizes dimeric Photosystem II. The psbK protein is also required for stability and assembly of Photosystem II.</td>
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<td>We will use this part to form a composite and test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640010" target="_blank">BBa_K1640010</a></td>
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<td>The psbO gene that comprises this part encodes a manganese stabilising protein.</td>
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<td>We will use this part to form a composite and test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640011" target="_blank">BBa_K1640011</a></td>
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<td>The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca<sub>2</sub><sup>+</sup> and Cl<sup>-</sup> cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.</td>
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<td>We will use this part to form a composite and test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640012" target="_blank">BBa_K1640012</a></td>
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<td>This part is composed of the psbQ and psbR genes. The psbQ protein is an oxygen enhancer protein. The psbR protein is an important link in the Photosystem II core complex for stable assembly of the Oxygen Evolving Complex.</td>
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<td>We will use this part to form a composite and test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>As of 27 August 2015 this part has been ordered but the sequence not confirmed.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640013" target="_blank">BBa_K1640013</a></td>
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<td>Encodes the PSII reaction centre D2 (Psb-D), PSII reaction centre D1 (Psb-A), and the PSII core monomer CP43 subunit (Psb-C).</td>
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<td>We will use this composite part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>PSII Operon 1.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640014" target="_blank">BBa_K1640014</a></td>
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<td>Encodes &Alpha;-subunit of the PSII cytochrome b559 (Psb-E), and subunits L (Psb-L), and J (Psb-J) of the CP43-less PSII core monomer.</td>
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<td>We will use this composite part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>PSII Operon 2.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640015" target="_blank">BBa_K1640015</a></td>
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<td>Encodes subunit T of the CP43-less PSII core monomer (Psb-T) and CP47 subunit of the PSII reaction centre (Psb-B).</td>
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<td>We will use this composite part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>PSII Operon 3.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640016" target="_blank">BBa_K1640016</a></td>
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<td>Encodes subunits M (Psb-M), Z (Psb-Z), H (psb-H), W (Psb-W), K (Psb-K) of the CP43-less PSII core monomer.</td>
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<td>We will use this composite part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>PSII Operon 4.</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1640017" target="_blank">BBa_K1640017</a></td>
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<td>Encodes subunits O (Psb-O), P (Psb-P), Q (Psb-Q) of the PSII oxygen evolving complex, and subunit R of the CP43-less PSII core monomer (Psb-R).</td>
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<td>We will use this composite part to test a minimal Photosystem 2 biosynthesis pathway.</td>
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<td>PSII Operon 5.</td>
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Latest revision as of 12:47, 19 November 2015

Parts

Part Documentation

We have designed and constructed 10 parts this year, 5 of which have been submitted to iGem HQ.

BBa_K1640018, aka ChlM, is a part integral to our synthetic chlorophyll biosynthesis pathway, which we have redesigned from earlier submissions, and have functionally characterised. This part has been submitted, and we are claiming a silver medal for this part.

ChlM is incorporated into the Chlorophyll a biosynthesis pathway in operon 3 as illustrated in the following diagram.



Parts BBa_K1640008, BBa_K1640020, BBa_K1640006 and BBa_K1640012 (respectively psbMZH, psbA, psbELJ, and psbQR) are 4 of the 10 biobricks we have designed for synthetic production of Chlamydomonas reinhardtii's photosystem II complex. These 4 parts have been submitted to iGem HQ and we are claiming our bronze medal for these new submissions.

These photosystem II biobricks are incorporated into the photosystem II biosynthesis pathway as illustrated in the following diagram.


PSII diagram

Part Table

<groupparts>iGEM015 Macquarie_Australia</groupparts>