Difference between revisions of "Team:UGA-Georgia/Measurement"

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<h2> Measurement</h2>
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<h2> Measurement Results</h2>
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<h3> Details of our Archaeal Interlab Study can be found on the <a href="https://2015.igem.org/Team:UGA-Georgia/Collaborations">Collaboration page</a>.</h3>
  
  
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<h4>Note</h4>
 
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Measurement Approach award</a>, you must fill out this page.</p>
 
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<p>There are a lot of exciting Parts in the Registry, but many Parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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<img src="https://static.igem.org/mediawiki/2015/f/fc/UGA-Georgia_Interlab_ESD.jpeg" width="800px";height="120px">
  
  
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<p><font size="3">Caption: ESD (Extreme Studentized Deviate) test to identify individual outlier data obtained for each mutant colony. The top row represents 10 mutant colonies, while each column represents triplicate mCherry fluorescence measurements from each team for the corresponding colony. Fluorescence values were standardized into percentage values relative to colony M3C1, by making the average fluorescence values for this colony 1 or 100%. Both significant and possible outliers were removed in subsequent analysis. N.A., not available. </p></font>
  
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<h4>Inspiration</h4>
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<p>You can look at what other teams did to get some inspiration! <br />
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<img src="https://static.igem.org/mediawiki/2015/4/44/UGA-Georgia_Interlab_After_ESD.jpeg" width="800px";height="120px">
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Here are a few examples:</p>
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<img src="https://static.igem.org/mediawiki/2015/c/c2/Interlab_before_ESD.jpg" width="800px";height="120px">
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen </a></li>
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<p><font size="3">Caption for upper table: Correlation matrix of mCherry fluorescence data across 7 participating iGEM teams after removing individual outlier data (see below for the ESD test table). Compared to the upper table, most of the R<sup>2</sup> values had been improved, indicating the outlier data did negatively affect the whole dataset. </p></font>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus">2014 Valencia Biocampus</a></li>
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<p><font size="3">Caption for lower table: : Correlation matrix of mCherry fluorescence data across 9 participating iGEM teams enabling identification of outlier data on the team level. The correlation between each pair of teams was assessed by plotting their respective fluorescence dataset against one another via a linear model, where a R<sup>2</sup> value was calculated to reflect how well the correlation was. As shown in the highlighted orange cells, datasets acquired by the two teams were systematically deviated from datasets obtained by all other teams. Therefore, the datasets from those two teams were removed in subsequent analysis. </p></font>
  
 
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Latest revision as of 13:24, 19 November 2015

Measurement Results

Details of our Archaeal Interlab Study can be found on the Collaboration page.

Caption: ESD (Extreme Studentized Deviate) test to identify individual outlier data obtained for each mutant colony. The top row represents 10 mutant colonies, while each column represents triplicate mCherry fluorescence measurements from each team for the corresponding colony. Fluorescence values were standardized into percentage values relative to colony M3C1, by making the average fluorescence values for this colony 1 or 100%. Both significant and possible outliers were removed in subsequent analysis. N.A., not available.

Caption for upper table: Correlation matrix of mCherry fluorescence data across 7 participating iGEM teams after removing individual outlier data (see below for the ESD test table). Compared to the upper table, most of the R2 values had been improved, indicating the outlier data did negatively affect the whole dataset.

Caption for lower table: : Correlation matrix of mCherry fluorescence data across 9 participating iGEM teams enabling identification of outlier data on the team level. The correlation between each pair of teams was assessed by plotting their respective fluorescence dataset against one another via a linear model, where a R2 value was calculated to reflect how well the correlation was. As shown in the highlighted orange cells, datasets acquired by the two teams were systematically deviated from datasets obtained by all other teams. Therefore, the datasets from those two teams were removed in subsequent analysis.