Difference between revisions of "Team:Peking/Basic Part"

 
(One intermediate revision by one other user not shown)
Line 13: Line 13:
 
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue/new?action=raw&amp;ctype=text/css " title="blue" media="screen" />
 
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue/new?action=raw&amp;ctype=text/css " title="blue" media="screen" />
 
   <style type="text/css">/*get rid of the annoying iGEM style*/
 
   <style type="text/css">/*get rid of the annoying iGEM style*/
−
#top_menu_inside, #top_menu_inside:before, #top_menu_inside:after { -webkit-box-sizing: content-box; -moz-box-sizing: border-box; box-sizing: content-box;}  
+
        #top_menu_inside, #top_menu_inside:before, #top_menu_inside:after { -webkit-box-sizing: content-box; -moz-box-sizing: border-box; box-sizing: content-box;}  
 
         #top_menu_under{font-size: 12px;}
 
         #top_menu_under{font-size: 12px;}
 
         #top_menu_14{font-size: 12px; height:18px}
 
         #top_menu_14{font-size: 12px; height:18px}
Line 42: Line 42:
 
   background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;padding:20px;padding-top:40px;
 
   background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;padding:20px;padding-top:40px;
 
}
 
}
−
body{
+
 
−
  text-align:justify;
+
−
}
+
 
     </style>
 
     </style>
 
</head>
 
</head>
Line 147: Line 145:
 
         <div class="row">
 
         <div class="row">
 
           <div class="col-md-6">
 
           <div class="col-md-6">
−
             <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >Parts</h2>
+
             <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2>
−
             <p style="margin-top:0px;font-size:14px" id="Overview">Each part give us a new experience.</p>
+
             <p style="margin-top:0px;font-size:14px" id="Overview">Each part gives us a new experience.</p>
 
           </div>
 
           </div>
 
           <div class="col-md-6">
 
           <div class="col-md-6">
Line 174: Line 172:
 
                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Parts">Parts Document</a>
+
                   <a href="https://2015.igem.org/Team:Peking/
 +
                  Parts">Parts Document</a>
 
                 </li>
 
                 </li>
−
               
 
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection" style="color:#00afd1">Parts Collection</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Basic_Part" style="color:#00afd1">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 
                 </li>
 
                 </li>
 
                
 
                
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
−
 
+
    <div id="sidebar2"class="widget widget-categories">
−
            <div id="sidebar2"class="widget widget-categories">
+
               <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
−
               <h4>Parts <span class="head-line"></span></h4>
+
 
                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Parts">Parts Document</a>
+
                   <a href="https://2015.igem.org/Team:Peking/
 +
                  Parts">Parts Document</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Basic_Part" style="color:#00afd1">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 
                 </li>
 
                 </li>
−
               
 
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection" style="color:#00afd1">Parts Collection</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 
                 </li>
 
                 </li>
 
                
 
                
Line 201: Line 216:
  
 
           <!-- Page Content -->
 
           <!-- Page Content -->
−
           <div class="col-md-9 page-content">
+
           <div class="col-md-9 page-content" style="text-align:justify;">
 
             <div id="Part-Favorite part" id="Document">
 
             <div id="Part-Favorite part" id="Document">
 
             <!-- Classic Heading -->
 
             <!-- Classic Heading -->
Line 243: Line 258:
  
 
                 <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
 
                 <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
−
<div style="margin-bottom:10px;">
+
<div><!--
−
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
+
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>-->
−
<!--<div class="col-md-12">
+
<div class="col-md-12">
 
   <table class="table table-hover table-striped" style="color:#666;font-size:14px">  
 
   <table class="table table-hover table-striped" style="color:#666;font-size:14px">  
 
<thead>
 
<thead>
Line 263: Line 278:
 
<td>Composite</td>  
 
<td>Composite</td>  
 
<td>sgRNA generator</td>  
 
<td>sgRNA generator</td>  
−
<td>WEI Weijia</td>
+
<td>LIU Wenchao, XU Luze</td>  
−
</tr>
+
−
 
+
−
<tr>
+
−
  <td>&nbsp;&radic;</td>
+
−
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
+
−
<td>Composite</td>
+
−
<td>Coding sequence of dCas9-Nluc</td>
+
−
<td>ZHANG Yihao</td>
+
 
</tr>  
 
</tr>  
  
Line 278: Line 285:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>Coding sequence Cluc-dCas9</td>  
+
<td>C-luc-dCas9</td>  
−
<td>ZHANG Yihao</td>  
+
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 
</tr>  
 
</tr>  
  
 
<tr>
 
<tr>
−
<td>&nbsp;</td>  
+
<td>&nbsp;&radic;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td>Compostie</td>  
 
<td>Compostie</td>  
−
<td>Coding sequence of N-luc-dCas9</td>  
+
<td>N-luc-dCas9</td>  
−
<td>ZHANG Yihao</td>
+
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 
</tr>  
 
</tr>  
  
Line 295: Line 302:
 
<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of STV-N-luc</td>  
 
<td>Coding sequence of STV-N-luc</td>  
−
<td>ZHANG Yihao</td>  
+
<td>LI Xiang, LI Jiaofeng</td>  
 
</tr>  
 
</tr>  
  
Line 303: Line 310:
 
<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of Cluc-STV</td>  
 
<td>Coding sequence of Cluc-STV</td>  
−
<td>ZHANG Yihao</td>  
+
<td>LI Xiang, LI Jiaofeng</td>  
 
</tr>  
 
</tr>  
  
Line 311: Line 318:
 
<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of Nluc416-FRB</td>
 
<td>Coding sequence of Nluc416-FRB</td>
−
<td>Xiang Li</td>   
+
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td>   
 
</tr>  
 
</tr>  
  
Line 319: Line 326:
 
<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of Nluc398-FRB</td>  
 
<td>Coding sequence of Nluc398-FRB</td>  
−
<td>Xiang Li</td>  
+
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td>
 
</tr>  
 
</tr>  
  
Line 327: Line 334:
 
<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of FKBP-Cluc398</td>  
 
<td>Coding sequence of FKBP-Cluc398</td>  
−
<td>Xiang Li</td>
+
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td>
 
</tr>  
 
</tr>  
  
Line 335: Line 342:
 
<td>Coding</td>  
 
<td>Coding</td>  
 
<td>Coding sequence of FKBP-Cluc394</td>
 
<td>Coding sequence of FKBP-Cluc394</td>
−
<td>Xiang Li</td>  
+
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td>
 
</tr>  
 
</tr>  
  
Line 342: Line 349:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>Coding sequence of dCas9-C-luc</td>
+
<td>dCas9-C-luc</td>
−
<td>ZHANG Yihao</td>  
+
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-N-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 
</tr>  
 
</tr>  
  
Line 350: Line 365:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>delta alpha segment of lacz fused with dCas9, His tag allows for protein purification</td>
+
<td>dCas9-delta alpha</td>
−
<td>Jiaofeng Li</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 358: Line 373:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
−
<td>N terminal of β-lactamase fused with dCas9</td>
+
<td>dCas9-Nlact</td>
−
<td>Jiaofeng Li</th>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 366: Line 381:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>  
 
<td>Compostie</td>  
 
<td>Compostie</td>  
−
<td>NFluc of lactamase fused with dCas9, His tag allows for protein purification</td>
+
<td>Nlact-dCas9</td>
−
<td>Jiaofeng Li</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 374: Line 389:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>dCas9 fused with C terminal of beta-lactamase</td>
+
<td>dCas9-Clact</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 382: Line 397:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>segment F[1,2] of DHFR-dCas9 fusion protein</td>
+
<td>F[1,2]-dCas9</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 390: Line 405:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>fusion protein of dCas9-F[1,2]</td>
+
<td>dCas9-F[1,2]</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 398: Line 413:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>DHFR F3 fragment-dCas9 fusion protein</td>
+
<td>F3-dCas9</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 406: Line 421:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>dCas9 fused with F3</td>
+
<td>dCas9-F3</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 414: Line 429:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>Δα segment of β-galactosidase fused with dCas9</td>
+
<td>delta alpha-dCas9</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
Line 422: Line 437:
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
−
<td>dCas9 fused with delta omega segment of β-galactosidase</td>
+
<td>dCas9-delta omega</td>
−
<td>Wei Weijia</td>  
+
<td>ZHANG Yihao, WEI Weijia</td>  
 
</tr>  
 
</tr>  
−
</table>  
+
</table>
−
</div>-->
+
</div>
  
 
                 <p><b>Parts for paired dCas9 reporter system</b></p>
 
                 <p><b>Parts for paired dCas9 reporter system</b></p>
  
−
                 <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>, FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>, Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a> and FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a> are used to validate the reconsitituation activity of split luciferase.</p>
+
                 <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the reconsitituation activity of split luciferase.</p>
−
                 <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>, Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>, dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a> and dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a> fusion proteins are used for constructing paired dCas9 reporter system.</p>
+
                 <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for constructing paired dCas9 reporter system.</p>
−
                 <p>&Delta;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>, dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>, Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>, dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>, F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>, dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,  dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>, dCas9-&Delta;&alpha;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>, &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a> are used for further development of dectection.</p>
+
                 <p>&Delta;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,<br/> dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-&Delta;&alpha;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>,<br/> &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development of dectection.</p>
 
                 <p><b>Parts for Molecular Beacon</b></p>
 
                 <p><b>Parts for Molecular Beacon</b></p>
−
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a> and Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a> are used for binding to molecular beacon.</p>
+
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
 
</div>
 
</div>
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
Line 443: Line 458:
 
                 <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3>
 
                 <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3>
 
<div>
 
<div>
−
                 <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explore a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the  form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p>
+
                 <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the  form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p>
 
                 <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p>
 
                 <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p>
 
                 <p>The Collection includes:</p>
 
                 <p>The Collection includes:</p>

Latest revision as of 00:40, 20 November 2015

Peking iGEM 2015

Parts

Each part gives us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator LIU Wenchao, XU Luze
 √ BBa_K1689009 Composite C-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
 √ BBa_K1689010 Compostie N-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689001 Coding Coding sequence of STV-N-luc LI Xiang, LI Jiaofeng
  BBa_K1689002 Coding Coding sequence of Cluc-STV LI Xiang, LI Jiaofeng
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689007 Composite dCas9-C-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689008 Composite dCas9-N-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689011 Composite dCas9-delta alpha ZHANG Yihao, WEI Weijia
  BBa_K1689012 Composite dCas9-Nlact ZHANG Yihao, WEI Weijia
  BBa_K1689013 Compostie Nlact-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689014 Coding dCas9-Clact ZHANG Yihao, WEI Weijia
  BBa_K1689015 Coding F[1,2]-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689016 Coding dCas9-F[1,2] ZHANG Yihao, WEI Weijia
  BBa_K1689017 Coding F3-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689018 Coding dCas9-F3 ZHANG Yihao, WEI Weijia
  BBa_K1689019 Coding delta alpha-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689020 Coding dCas9-delta omega ZHANG Yihao, WEI Weijia

Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003),
FKBP-Cluc398 (BBa_K1689005),
Nluc398-FRB (BBa_K1689004),
FKBP-Cluc394 (BBa_K1689006)
are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010),
Cluc-dCas9(BBa_K1689009),
dCas9-Nluc (BBa_K1689008),
dCas9-Cluc (BBa_K1689007)
fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011),
dCas9-Nlact (BBa_K1689012),
Nlact-dCas9 (BBa_K1689013),
dCas9-Clact (BBa_K1689014),
F[1,2]-dCas9 (BBa_K1689015),
dCas9-F[1,2] (BBa_K1689016),
F3-dCas9 (BBa_K1689017),
dCas9-F3 (BBa_K1689018),
dCas9-Δα (BBa_K1689019),
Δω-dCas9 (BBa_K1689020)
are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001),
Cluc-STV (BBa_K1689002)
are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.