Difference between revisions of "Team:Peking/Composite Part"

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{{Peking}}
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  <meta charset="utf-8">
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    /* to style the sidebar*/
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<h2> Composite Parts</h2>
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    </style>
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</head>
  
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<body>
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  <!-- Container -->
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  <div id="container">
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    <!-- Start Header -->
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    <header class="clearfix">
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        <!-- Start  Logo & Naviagtion  -->
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            <div class="navbar navbar-default navbar-top">
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                <div class="container">
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                    <div class="navbar-header" >
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                        <!-- Stat Toggle Nav Link For Mobiles -->
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                        <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse" style="margin-top:40px">
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                            <i class="fa"><img  style="margin-left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-1.png" ></i>
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                        </button>
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                        <!-- End Toggle Nav Link For Mobiles -->
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                        <a class="navbar-brand" href="https://2015.igem.org/Team:Peking">
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                            <img alt="Peking iGEM 2015" src="https://static.igem.org/mediawiki/2015/8/8b/Peking-header-logo-1.png" style="height:55px;margin-top:7px">
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                        </a>
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                    </div>
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                    <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
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                        <!-- Start Navigation List -->
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                        <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;padding-top:10px">
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a>
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                                <ul class="dropdown">
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                                    <li><a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
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                                    </li>
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                                    <li><a class="active" href="https://2015.igem.org/Team:Peking/Parts">Parts</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/Design">Project</a>
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                                <ul class="dropdown">
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                                    <li><a href="https://2015.igem.org/Team:Peking/Design">Overview</a>
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                                    </li>
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<li><a href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired<span style="text-transform:lowercase"> d</span>Cas9 Reporter</a>                                   
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</li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Isothermal">Iso-<span style="text-transform:lowercase">t</span>hermal Amplification</a></li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Device">Hardware</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Speculation">Speculation</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/Modeling">Modeling</a>
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                                <ul class="dropdown">
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Modeling">Array Design</a>
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                                    </li>
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                                    <li><a href="https://2015.igem.org/Team:Peking/Modeling/Analysis">Analysis algorithm</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li>
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                                <a href="https://2015.igem.org/Team:Peking/Practices">Practices</a>
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                                <ul class="dropdown">
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices">Overview</a>
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                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Background">TB Facts</a>
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                                    </li>
 +
                                    <li><a  href="https://2015.igem.org/Team:Peking/Practices/Consultation">Consultation and Interview</a>
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                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Engagement">Public Engagement</a>
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                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Outreach">Ethics and Economics Issues</a>
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                                    </li>
 +
                                </ul>
 +
                            </li>
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                            <li>
 +
                                <a href="https://2015.igem.org/Team:Peking/Team">Lab</a>
 +
                                <ul>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Team">Team</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Notebook">Notebook</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Attributions">Attributions</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Safety">Safety</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
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                        </ul>
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                        <!-- End Navigation List -->
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                    </div>
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                </div>
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            </div>
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            <!-- End Header Logo & Naviagtion -->
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        </header>
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        <!-- End Header Section -->
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    <!-- Start Page Banner -->
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    <div class="page-banner">
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      <div class="container">
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        <div class="row">
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          <div class="col-md-6">
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            <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2>
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            <p style="margin-top:0px;font-size:14px" id="Overview">Each part gives us a new experience.</p>
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          </div>
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          <div class="col-md-6">
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            <ul class="breadcrumbs">
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              <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
 +
              <li>Parts</li>
 +
            </ul>
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          </div>
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        </div>
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      </div>
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    </div>
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    <!-- End Page Banner -->
  
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<div class="highlightBox">
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<h4>Note</h4>
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    <!-- Start Content -->
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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    <div id="content">
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</div>
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      <div class="container">
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        <div class="row blog-page">
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          <!--Sidebar-->
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          <div class="col-md-3 sidebar left-sidebar">
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            <!-- Categories Widget -->
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            <div id="sidebar1"class="widget widget-categories">
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              <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
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                <ul>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/
 +
                  Parts">Parts Document</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part" style="color:#00afd1">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 +
                </li>
 +
             
 +
              </ul>
 +
            </div>
 +
<div id="sidebar2"class="widget widget-categories">
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              <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
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                <ul>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/
 +
                  Parts">Parts Document</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part" style="color:#00afd1">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 +
                </li>
 +
             
 +
              </ul>
 +
            </div>
 +
          </div>
 +
          <!--End sidebar-->
  
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<p>
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          <!-- Page Content -->
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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          <div class="col-md-9 page-content" style="text-align:justify;">
 +
            <div id="Part-Favorite part" id="Document">
 +
            <!-- Classic Heading -->
 +
              <h3 class="classic-title" style="margin-top:50px"><span>Favorite Parts</span></h3>
 +
<div>
 +
              <p><b>Nluc-dCas9 fusion protein</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/a/a2/Peking-Parts-ND-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-2"></div>
 +
          </div>
 +
              <p>Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a> and sgRNAs, our paired dCas9 reporter system were constructed.</p>
 +
             
 +
              <p><b>Cluc-dCas9 fusion protein</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/3/32/Peking-Parts-CD-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 +
              <p>Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a> and sgRNAs, our paired dCas9 reporter system were constructed.</p>
 +
             
 +
              <p><b>sgRNA generator</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/f/f2/Peking-Parts-gRNA-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 +
              <p> sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.  
 
</p>
 
</p>
 +
             
 +
              </div>
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
                <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
 +
<div><!--
 +
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>-->
 +
<div class="col-md-12">
 +
  <table class="table table-hover table-striped" style="color:#666;font-size:14px">
 +
<thead>
 +
<tr>
 +
<th>Favorite</th>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Design</th>
 +
</tr>
 +
</thead>  
  
 +
<tbody>
 +
<tr>
 +
  <td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">BBa_K1689000</a></td>
 +
<td>Composite</td>
 +
<td>sgRNA generator</td>
 +
<td>LIU Wenchao, XU Luze</td>
 +
</tr>
  
 +
<tr>
 +
  <td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>
 +
<td>Composite</td>
 +
<td>C-luc-dCas9</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>
 +
<td>Compostie</td>
 +
<td>N-luc-dCas9</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">BBa_K1689001</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of STV-N-luc</td>
 +
<td>LI Xiang, LI Jiaofeng</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">BBa_K1689002</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Cluc-STV</td>
 +
<td>LI Xiang, LI Jiaofeng</td>
 +
</tr>
 +
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">BBa_K1689003</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc416-FRB</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1">BBa_K1689004</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc398-FRB</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">BBa_K1689005</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc398</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1">BBa_K1689006</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc394</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-C-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-N-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-delta alpha</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-Nlact</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>
 +
<td>Compostie</td>
 +
<td>Nlact-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-Clact</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>
 +
<td>Coding</td>
 +
<td>F[1,2]-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-F[1,2]</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>
 +
<td>Coding</td>
 +
<td>F3-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-F3</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>
 +
<td>Coding</td>
 +
<td>delta alpha-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-delta omega</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
</table>
 
</div>
 
</div>
  
 +
                <p><b>Parts for paired dCas9 reporter system</b></p>
 +
 +
                <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the reconsitituation activity of split luciferase.</p>
 +
                <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for constructing paired dCas9 reporter system.</p>
 +
                <p>&Delta;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,<br/>  dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-&Delta;&alpha;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>,<br/> &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development of dectection.</p>
 +
                <p><b>Parts for Molecular Beacon</b></p>
 +
                <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
 +
</div>
 +
                <p id="Collection"><b>Parts for sgRNA generation</b></p>
 +
<div>
 +
                <p>sgRNA generator <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a> is used for generating sgRNA</p>
 +
             
 +
</div>
 +
                <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3>
 +
<div>
 +
                <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the  form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p>
 +
                <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p>
 +
                <p>The Collection includes:</p>
 +
 +
                <p><b>dCas9-split luciferase Parts</b></p>
 +
                <p>Nluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a><br/>
 +
                Cluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a><br/>
 +
                dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a><br/>
 +
                dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a></p>
 +
 +
                <p><b>dCas9-split Dihydrofolate Reductase Parts</b></p>
 +
                <p>F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a><br/>
 +
                F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689017)</a><br/>
 +
                dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a><br/>
 +
                dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a></p>
 +
 +
                <p><b>dCas9-split β–Lactamase Parts</b></p>
 +
                <p>dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a><br/>
 +
                Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">(BBa_K1689013)</a><br/>
 +
                dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a></p>
 +
               
 +
                <p><b>dCas9-splitβ–Galactosidase Parts</b></p>
 +
                <p>&Delta;&alpha;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">(BBa_K1689011)</a><br/>
 +
                dCas9-&Delta;&alpha; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">(BBa_K1689019)</a><br/>
 +
                &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a></p>
 +
                <p>Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.</p>
 +
                </div>
 +
 +
                </div>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 +
 +
 +
 +
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Latest revision as of 00:41, 20 November 2015

Peking iGEM 2015

Parts

Each part gives us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator LIU Wenchao, XU Luze
 √ BBa_K1689009 Composite C-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
 √ BBa_K1689010 Compostie N-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689001 Coding Coding sequence of STV-N-luc LI Xiang, LI Jiaofeng
  BBa_K1689002 Coding Coding sequence of Cluc-STV LI Xiang, LI Jiaofeng
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689007 Composite dCas9-C-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689008 Composite dCas9-N-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689011 Composite dCas9-delta alpha ZHANG Yihao, WEI Weijia
  BBa_K1689012 Composite dCas9-Nlact ZHANG Yihao, WEI Weijia
  BBa_K1689013 Compostie Nlact-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689014 Coding dCas9-Clact ZHANG Yihao, WEI Weijia
  BBa_K1689015 Coding F[1,2]-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689016 Coding dCas9-F[1,2] ZHANG Yihao, WEI Weijia
  BBa_K1689017 Coding F3-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689018 Coding dCas9-F3 ZHANG Yihao, WEI Weijia
  BBa_K1689019 Coding delta alpha-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689020 Coding dCas9-delta omega ZHANG Yihao, WEI Weijia

Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003),
FKBP-Cluc398 (BBa_K1689005),
Nluc398-FRB (BBa_K1689004),
FKBP-Cluc394 (BBa_K1689006)
are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010),
Cluc-dCas9(BBa_K1689009),
dCas9-Nluc (BBa_K1689008),
dCas9-Cluc (BBa_K1689007)
fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011),
dCas9-Nlact (BBa_K1689012),
Nlact-dCas9 (BBa_K1689013),
dCas9-Clact (BBa_K1689014),
F[1,2]-dCas9 (BBa_K1689015),
dCas9-F[1,2] (BBa_K1689016),
F3-dCas9 (BBa_K1689017),
dCas9-F3 (BBa_K1689018),
dCas9-Δα (BBa_K1689019),
Δω-dCas9 (BBa_K1689020)
are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001),
Cluc-STV (BBa_K1689002)
are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.