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<h2>UNITN <span style="text-transform:lowercase;">i</span>GEM 2015</h2>
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<p><strong>Solar pMFC</strong><br/>A microbial fuel cell with <br/>a light-driven <strong><span style="font-style:italic">E. coli</span> engine</strong></p>
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<span class="title"> UNITN-Trento
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<h2>Electron production with<br /><span style="font-weight:500; font-style:italic;">E. coli</span> and Proteorhodopsin </h2>
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iGEM 2015 </span>
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<p style="width:80%; text-align:left; margin:auto;">We are the UNITN-Trento iGEM team 2015 and here is our wonderful idea to change the way people think about Microbial Fuel Cells. We attempted to improve the MFC platform by exploiting the power of the sun and the versatility of <span class="bacterium" >Escherichia coli</span>, so we added new interesting features to build a “Solar pMFC”. We presented our work in Boston at the iGEM Giant Jamboree and our work  was nominated among the three top energy projects worldwide. Check out our wiki page to learn more!</p><br /><br /><br />
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<br /><span style="font-style:italic; color:#555; font-size:1.3em;"> Electron-production with engineered E.coli and proteorhodopsin</span>
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<p class="pintest">Electricity from <i>E. coli</i><br/>
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<span>Check Out our Amazing Idea! Have Fun!<br/>
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<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Description">Project</a></span></p>
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<p class="pintest">iGEM UNITN-Trento: The Giant Jamboree 2015 <br/>
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<span> We presented our work in Boston at the iGEM Giant Jamboree and our work  was nominated among the three top energy projects worldwide!<br/>
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<a class="btn" href="https://2015.igem.org/Results" target="_blank"> iGEM 2015 Results </a></span></p>
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<p class="pintest">iGEM UNITN-Trento: The Giant Jamboree 2015 <br/>
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<span> We presented our work in Boston at the iGEM Giant Jamboree and our work was nominated among the three top energy projects worldwide!<br/>
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<a class="btn" href="https://2015.igem.org/Results" target="_blank"> iGEM 2015 Results </a></span></p>
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<p class="pintest">UNITN-iGEM Team 2015<br/>
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<span>Click here to meet the team!<br/>
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<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Team"> Team </a></span></p>
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<p class="pintest">InterLab Measurement Study<br/>
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<span>Many Techniques, Different Strains and so much Fluorescence: we were nominated for the Best Innovation in Measurement Award!<br/>
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<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Measurement"> Interlab Study </a></span></p>
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<p class="pintest">Energy Waste is a Serious Problem<br/>
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<span>Follow Us and Learn How to Save It<br/>
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<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Practices"> Human Practices </a></span></p>
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<p class="pintest">Safety First<br />
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<span>We Do Care about Safety: Check It Out!<br/>
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<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Safety"> Safety </a></span></p>
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<p class="pintest">Synthetic Biology and Mountains<br/>
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<span>Admire our Lovely City, Surrounded by Mountains. Trento is Waiting for You<br/>
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<a class="btn" href="http://www.unitn.it/en" target="_blank"> University of Trento </a></span></p>
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<h2>Achievements</h2>
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<p>The aim of our project is to develop a pseudoautotrophic <i>E. coli</i>, enabling it to live in the <span class="highlight">microbial fuel cell</span> anaerobic condition. Our idea is to engineer <span class="highlight"><i>E. coli</i></span> with <span class="highlight"><i>proteorhodopsin</i></span>, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with <i>blh</i>, that encodes for the enzyme that cleaves beta-carotene and produces retinal.</p>
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<p>To increase electron production we plan to overexpress <i>pncB</i> that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production.</p>
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<h4><strong>Gold</strong> medal</h4>
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<p>We were awarded with the iGEM Gold Medal, for successfully satisfying the medal criteria</p>
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<p>Our bacterial device will give rise to a <span class="highlight">self-sustainable system</span>, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.</p>
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<h4><strong>Nomination</strong>: Best Energy Project</h4>
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<p>We were among the three teams nominated for the <strong>Best energy project</strong> Award!</p>
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<h4><strong>Nomination</strong>: Best Innovation in Measurement Award</h4>
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<p> we were nominated for the <strong>Best Innovation in Measurement</strong> (undergraduate) Award!</p>
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<h4><span class="enphatext">7</span> new BioBricks</h4>
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<p>We built 7 new BioBricks. Check out our <a href="https://2015.igem.org/Team:UNITN-Trento/Parts" class="i_linker">Parts</a> page to know more!</p>
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<h4>Improved <span class="enphatext">1</span> Part</h4>
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<p>Something existing, reshaped better. <a href="https://2015.igem.org/Team:UNITN-Trento/Results/Proteorhodopsin" class="i_linker">Proteorhodopsin</a> works better now!</p>
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<h4>Measurements Interlab Study</h4>
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<p>We are participating also to the Measurements Interlab Study. Check out our progress in the <a href="https://2015.igem.org/Team:UNITN-Trento/Measurement" class="i_linker"> Interlab</a> Section!</p>
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Latest revision as of 14:29, 20 November 2015

Electron production with
E. coli and Proteorhodopsin


We are the UNITN-Trento iGEM team 2015 and here is our wonderful idea to change the way people think about Microbial Fuel Cells. We attempted to improve the MFC platform by exploiting the power of the sun and the versatility of Escherichia coli, so we added new interesting features to build a “Solar pMFC”. We presented our work in Boston at the iGEM Giant Jamboree and our work was nominated among the three top energy projects worldwide. Check out our wiki page to learn more!







Achievements

Gold medal

We were awarded with the iGEM Gold Medal, for successfully satisfying the medal criteria

Nomination: Best Energy Project

We were among the three teams nominated for the Best energy project Award!

Nomination: Best Innovation in Measurement Award

we were nominated for the Best Innovation in Measurement (undergraduate) Award!

7 new BioBricks

We built 7 new BioBricks. Check out our Parts page to know more!

Improved 1 Part

Something existing, reshaped better. Proteorhodopsin works better now!

Measurements Interlab Study

We are participating also to the Measurements Interlab Study. Check out our progress in the Interlab Section!