Difference between revisions of "Team:DTU-Denmark"

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{{DTU-Denmark}}
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<html>
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<html>
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  <head>
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<h2> Welcome to iGEM 2015! </h2>
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    <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
  
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<h4>Before you start: </h4>
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      <link rel=stylesheet href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css">
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<p> Please read the following pages:</p>
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      <link rel=stylesheet href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css">
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<ul>
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      <link rel=stylesheet href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css">
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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      <link rel=stylesheet href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css">
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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      <link rel=stylesheet href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css">
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</ul>
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      <link rel=stylesheet href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css">
  
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<div class="highlightBox">
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    <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
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<h4> Styling your wiki </h4>
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    <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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    <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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    <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
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</div>
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<h4> Editing your wiki </h4>
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    <!-- MathJax (LaTeX for the web) -->
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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    <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:DTU-Denmark&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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  </head>
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  <body data-spy="scroll" data-target="#scrollspy" data-offset="90">
 +
    <div id="fb-root"></div>
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    <script>(function(d, s, id) {
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      var js, fjs = d.getElementsByTagName(s)[0];
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      if (d.getElementById(id)) return;
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      js = d.createElement(s); js.id = id;
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      js.src = "//connect.facebook.net/da_DK/all.js#xfbml=1&version=v2.4";
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      fjs.parentNode.insertBefore(js, fjs);
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    }(document, 'script', 'facebook-jssdk'));</script>
 +
    <nav class="navbar navbar-default navbar-fixed-top" role="navigation">
 +
      <!-- Brand and toggle get grouped for better mobile display -->
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      <div class="container-fluid">
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        <div class="navbar-header">
 +
          <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
 +
            <span class="sr-only">Toggle navigation</span>
 +
            <i class="fa fa-bars"></i>
 +
          </button>
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          <a class="navbar-brand navimg" href="#"><img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png"></a>
 +
        </div>
 +
        <!-- Collect the nav links, forms, and other content for toggling -->
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        <div class="collapse navbar-collapse" id="nav-collapse">
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          <ul class="nav navbar-nav">
 +
                <li class="active">
 +
                <a href="/Team:DTU-Denmark"
 +
                  >Home
 +
                  </a></li>
 +
                <li
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                >
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                <a href=""
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                  class="dropdown-toggle"
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                  data-toggle="dropdown"
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                  role="button"
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                  aria-expanded="false">Team and Attributions
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                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
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                <a href="/Team:DTU-Denmark/Team"
 +
                  >Team
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                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Attributions"
 +
                  >Attributions
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                  </a></li></ul></li>
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                <li
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                >
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                <a href=""
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                  class="dropdown-toggle"
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                  data-toggle="dropdown"
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                  role="button"
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                  aria-expanded="false">Project
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                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Overview"
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                  >Overview
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                  </a></li>
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                <li >
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                <a href="/Team:DTU-Denmark/Project/Background"
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                  >Background
 +
                  </a></li>
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                <li >
 +
                <a href="/Team:DTU-Denmark/Project/MAGE"
 +
                  >MAGE subtilis
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Tyrocidine"
 +
                  >Tyrocidine
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Chip"
 +
                  >Lab-on-a-disc
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                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Inteins"
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                  >Inteins
 +
                  </a></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Project/Detection"
 +
                  >Detection of NRP
 +
                  </a></li></ul></li>
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Practices"
 +
                  >Human Practices
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                  </a></li>
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                <li
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                >
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                <a href=""
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                  class="dropdown-toggle"
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                  data-toggle="dropdown"
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                  role="button"
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                  aria-expanded="false">Parts Collection
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                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Parts"
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                  >Parts
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                  </a></li>
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                <li >
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                <a href="/Team:DTU-Denmark/Description"
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                  >Characterisation of xylR
 +
                  </a></li></ul></li>
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                <li >
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                <a href="/Team:DTU-Denmark/Journal"
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                  >Journal
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                  </a></li>
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                <li >
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                <a href="/Team:DTU-Denmark/Software"
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                  >Software
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                  </a></li>
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                <li
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                >
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                <a href=""
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                  class="dropdown-toggle"
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                  data-toggle="dropdown"
 +
                  role="button"
 +
                  aria-expanded="false">Achievements
 +
                  <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
                <li >
 +
                <a href="/Team:DTU-Denmark/Achievements"
 +
                  >Key Achievements
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                  </a></li>
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                <li >
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                <a href="/Team:DTU-Denmark/Collaborations"
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                  >Collaborations
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                  </a></li>
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                <li >
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                <a href="/Team:DTU-Denmark/Judging_Form"
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                  >Judging Form
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                  </a></li></ul></li>
 +
            <li class="hidden-xs">
 +
              <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
 +
                <i class="fa fa-facebook"></i><span class="visible-xs"> Follow us on Facebook</span>
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              </a>
 +
            </li>
 +
            <li class="visible-xs"><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
 +
            <li class="hidden-xs">
 +
              <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
 +
                <i class="fa fa-twitter"></i>
 +
              </a>
 +
            </li>
 +
            <li class="visible-xs"><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
 +
            <li class="hidden-lg">
 +
              <a href="https://igem.org" target="_blank" class="navimg">
 +
                <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
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              </a>
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            </li>
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          </ul>
 +
        </div><!-- /.navbar-collapse -->
 +
      </div>
 +
    </nav>
  
  
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<h4>Templates </h4>
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<nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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  <div class="container-fluid" id="scrollspy">
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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    <ul class="nav navbar-nav">
 +
      <li><a class="page-scroll" href="#ProjectDescription">Project Description</a></li><li><a class="page-scroll" href="#SocialMedia">Social Media</a></li>
 +
    </ul>
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  </div>
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</nav>
  
  
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<h4>Tips</h4>
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<div class="jumbotron hero" style="background-image: url(https://static.igem.org/mediawiki/2015/0/0b/DTU-Denmark_hero.jpg)">
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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      <div class="igem-logo visible-lg">
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<ul>
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        <a href="https://igem.org" target="_blank">
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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          <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
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<li>Be clear about what you are doing and how you plan to do this.</li>
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        </a>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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      </div>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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      <div class="container-fluid">
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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        <div class="row hero-text">
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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          <div class="col-md-12">
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<li>Have lots of fun! </li>
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</ul>  
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<h3>Technical University of Denmark</h3>
 +
<h1>The Synthesizer</h1>
  
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            <hr/>
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            <ul class="list-inline hidden-xs hidden-sm">
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              <li>
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                <a id="ProjectDescription-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#ProjectDescription">Project Description</a>
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              </li>
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              <li>
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                <a id="SocialMedia-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#SocialMedia">Social Media</a>
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              </li>
 +
             
 +
            </ul>
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            <ul class="list-inline visible-xs visible-sm">
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 +
              <li>
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                <a id="ProjectDescription-submenu" class="page-scroll" href="#ProjectDescription">Project Description</a>
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              </li>
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 +
              <li>
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                <a id="SocialMedia-submenu" class="page-scroll" href="#SocialMedia">Social Media</a>
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              </li>
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 +
            </ul>
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 +
           
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          </div>
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        </div>
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 +
        <div class="row">
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          <div class="col-md-12">
 +
            Scroll down for more<br>
 +
            <a href="#ProjectDescription" class="btn btn-circle btn-transparent page-scroll">
 +
              <i class="fa fa-angle-double-down"></i>
 +
            </a>
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          </div>
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        </div>
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      </div>
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    </div>
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 +
   
 +
       
 +
<div id="ProjectDescription">
 +
  <div class="container">
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 +
    <div class="row col-md-12">
 +
      <h1>
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        Project Description
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      </h1>
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        <p style="text-align: center;"><span style="font-size:72px;"><span style="font-size:72px;"><span class="fa fa-headphones" style="color:rgb(0, 0, 0);"></span></span></span></p>
  
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<h4>Inspiration</h4>
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<p style="text-align: center;">Listen to our podcast about our <a href="https://static.igem.org/mediawiki/2015/5/50/Podcast_project.mp4"><em>Project</em></a>&nbsp;or&nbsp;<a href="https://static.igem.org/mediawiki/2015/f/f5/Podcast_education.mp4"><em>Human practises</em></a></p>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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−
<ul>
+
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
  
−
<h4> Uploading pictures and files </h4>
+
<p>&nbsp;</p>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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−
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
  
−
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
+
<p><img alt="" src="https://static.igem.org/mediawiki/2015/5/5e/DTU-Denmark_gold.png" style="float:left; margin-right:10px;" /></p>
  
 +
<p><br />
 +
DTU Denmark received a gold medal at the Giant Jamboree in Boston 2015 and a nomination for best educational human practises. Click on the headphones to hear our podcasts explaining our project and our human practises.</p>
  
 +
<p>We also designed a <a href="https://2015.igem.org/Team:DTU-Denmark/Software">Wiki Wizard</a> that allows you to easily create and design wikis without knowing about HTML, CSS, and JavaScript. By using the shared editing mode, team members can edit the Wiki at the same time - just like Google Docs.</p>
  
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</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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<p>&nbsp;</p>
  
 +
<p>Nonribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.</p>
 +
 +
<p style="text-align: center;"><img alt="" src="https://static.igem.org/mediawiki/2015/7/7d/DTU-Denmark_drawingpng.png" style="width: 625px; height: 378px;" /></p>
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<p>&nbsp;</p>
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    </div>
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 +
  </div>
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</div>
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 +
       
 +
<div id="SocialMedia">
 +
  <div class="container">
 +
 
 +
    <div class="row col-md-12">
 +
      <h1>
 +
        Social Media
 +
      </h1>
 +
       
 +
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Latest revision as of 23:51, 20 November 2015

Technical University of Denmark

The Synthesizer

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Project Description

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DTU Denmark received a gold medal at the Giant Jamboree in Boston 2015 and a nomination for best educational human practises. Click on the headphones to hear our podcasts explaining our project and our human practises.

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Nonribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.

 

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DTU Biobuilders
Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
P: +45 45 25 25 25
M: dtu-igem-2015@googlegroups.com