Difference between revisions of "Team:William and Mary/Notebook"
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<li> | <li> | ||
<a href="https://2015.igem.org/Team:William_and_Mary/Measurement"> | <a href="https://2015.igem.org/Team:William_and_Mary/Measurement"> | ||
− | + | Results | |
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:William_and_Mary/Modeling"> | ||
+ | Modeling | ||
</a> | </a> | ||
</li> | </li> | ||
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<a href="https://2015.igem.org/Team:William_and_Mary/Practices"> | <a href="https://2015.igem.org/Team:William_and_Mary/Practices"> | ||
Human Practices | Human Practices | ||
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</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
<a href="https://2015.igem.org/Team:William_and_Mary#ref"> | <a href="https://2015.igem.org/Team:William_and_Mary#ref"> | ||
− | + | References | |
</a> | </a> | ||
</li> | </li> | ||
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<a href="https://2015.igem.org/Team:William_and_Mary/Medal_Criteria"> | <a href="https://2015.igem.org/Team:William_and_Mary/Medal_Criteria"> | ||
Medal Criteria | Medal Criteria | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:William_and_Mary/Team"> | ||
+ | Team | ||
</a> | </a> | ||
</li> | </li> | ||
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<a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:dCas9"><img alt="..." src="https://static.igem.org/mediawiki/2015/1/18/WMElectroporationIconColor.png" height= "100" width= "120"/><a> | <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:dCas9"><img alt="..." src="https://static.igem.org/mediawiki/2015/1/18/WMElectroporationIconColor.png" height= "100" width= "120"/><a> | ||
</div> | </div> | ||
− | <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:dCas9"><h3> | + | <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:dCas9"><h3>Repression</h3></a> |
<p>Repressing with dCas9</p> | <p>Repressing with dCas9</p> | ||
</div> | </div> | ||
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<div class="project"> | <div class="project"> | ||
<img alt="..." src="https://static.igem.org/mediawiki/2015/4/41/WMSeptember.jpg"> | <img alt="..." src="https://static.igem.org/mediawiki/2015/4/41/WMSeptember.jpg"> | ||
− | <a class="over-area color-black " href="javascript:void(0)" onclick="rubik.showModal(this)" data-target=" | + | <a class="over-area color-black " href="javascript:void(0)" onclick="rubik.showModal(this)" data-target="september"> |
<div class="content"> | <div class="content"> | ||
<h3>September</h3> | <h3>September</h3> | ||
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<li>Confirmed that dCas9 and our gRNA design works</li> | <li>Confirmed that dCas9 and our gRNA design works</li> | ||
<li>Assembled the CFP under R0010 with KanR, so that it can be PCR’d with the integration PCRs, and integrated</li> | <li>Assembled the CFP under R0010 with KanR, so that it can be PCR’d with the integration PCRs, and integrated</li> | ||
− | <li>Attempted to make TetR operon</li></ | + | <li>Attempted to make TetR operon</li></ul> |
<h3>Week 3 (7/13-7/19)</h3> | <h3>Week 3 (7/13-7/19)</h3> | ||
<ul type="circle"><li>Attempted to test our integration protocol, by integrating KanR onto the E. coli genome</li> | <ul type="circle"><li>Attempted to test our integration protocol, by integrating KanR onto the E. coli genome</li> | ||
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<ul type="circle"><li>Continued assembling CFP and YFP operons</li> | <ul type="circle"><li>Continued assembling CFP and YFP operons</li> | ||
<li>Began assembling CFP operons with KanR (to integrate)</li> | <li>Began assembling CFP operons with KanR (to integrate)</li> | ||
− | <li>Continued attempts to make TetR operon</li></ul> | + | <li>Continued attempts to make TetR operon</li></ul><br> |
<h2>8/10-8/23: School mandates that we leave</h2> | <h2>8/10-8/23: School mandates that we leave</h2> | ||
<ul type="circle"><li>During this time, we learned that TetR had been mislabelled, and the part we were actually looking to assemble to confer tetracycline resistance was “TetA”</li> | <ul type="circle"><li>During this time, we learned that TetR had been mislabelled, and the part we were actually looking to assemble to confer tetracycline resistance was “TetA”</li> | ||
− | <li>We designed PCRs to assemble TetA</li></ul> | + | <li>We designed PCRs to assemble TetA</li></ul><br> |
<h3>Week 4 (8/24-8/30)</h3> | <h3>Week 4 (8/24-8/30)</h3> | ||
<ul type="circle"><li>Continued working on Interlab Study</li> | <ul type="circle"><li>Continued working on Interlab Study</li> | ||
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</div> | </div> | ||
</div> | </div> | ||
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+ | <div class="project-content" id="september"> | ||
+ | <div> | ||
+ | <div class="project-details"> | ||
+ | <span class="icon-close"><i class="pe-7s-close-circle"></i></span> | ||
+ | <div class="container"> | ||
+ | <div class="project-title"> | ||
+ | <h1>September</h1> | ||
+ | <div class="separator-container"> | ||
+ | <div class="separator line-separator">✻</div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="article"> | ||
+ | <div class="project-text"> | ||
+ | <h3>Week 1 (8/31-9/6)</h3> | ||
+ | <ul type="circle"> | ||
+ | <li>Integrated CFP operons with KanR</li> | ||
+ | <li>Integrated YFP operons with TetA into E. coli containing integrated CFPs</li></ul> | ||
+ | <h3>Week 2 (9/7-9/13)</h3> | ||
+ | <ul type="circle"> | ||
+ | <li>Continued attempts to integrate YFPs into E. coli containing integrated CFPs</li> | ||
+ | <li>Imaged successful double integrations for R0010, R0011, R0051</li> | ||
+ | <li>Analyzed data from double integrations</li></ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
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<div class="body-layer"></div> | <div class="body-layer"></div> | ||
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+ | <footer class="footer footer-color-black" data-color="black"> | ||
+ | <div class="container"> | ||
+ | <nav class="pull-left navbar-burger"> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:William_and_Mary"> | ||
+ | Home | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="mailto:igem@wm.edu"> | ||
+ | Contact | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <div class="social-area pull-right"> | ||
+ | <a href="https://www.facebook.com/wmigem2015"> | ||
+ | <i class="fa fa-facebook-square"></i> | ||
+ | </a> | ||
+ | <a href="https://twitter.com/wmigem"> | ||
+ | <i class="fa fa-twitter"></i> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="copyright"> | ||
+ | © 2015 William & Mary iGEM | ||
+ | </div> | ||
+ | </div> | ||
+ | </footer> | ||
+ | </div> | ||
Latest revision as of 04:37, 21 November 2015
Our Notebook On this page you can view the work we did each month, from June through September. You can also find our protocols. Important Protocols Easy access to our most complex protocols.
June
Week 1 (6/1-6/7)
Week 2 (6/8-6/14)
Week 3 (6/15-6/21)
Week 4 (6/22-6/28)
July
Week 1 (6/29-7/5)
Week 2 (7/6-7/12)
Week 3 (7/13-7/19)
Week 4 (7/20-7/26)
Week 5 (7/26-8/2)
August
Week 1 (8/3-8/9)
8/10-8/23: School mandates that we leave
Week 4 (8/24-8/30)
September
Week 1 (8/31-9/6)
Week 2 (9/7-9/13)