Difference between revisions of "Team:William and Mary/Part Collection"

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{{William_and_Mary}}
 
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<h2> Part Collection</h2>
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<html lang="en">
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        <title>NOISE - W&M iGEM</title>
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Part Collection award</a>, you must fill out this page.</p>
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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                                    <p class="h2WM">Part Collection</p>
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<p>In keeping with our theme of promoter effects on transcription, we created a suite of guide RNAs for the targeted dCas9 repression of 11 of the most frequently used promoters in the Registry. For cells which express a dCas9 protein, the transformation of any of our guide RNA parts will repress the expression of any protein under the associated promoter. We also included a scrambled guide RNA to serve as a negative control for troubleshooting purposes.
  
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<p>(BELOW) An example of repression: fluorescence values for RFP under the R0010 promoter, in cells with and without the R0010 guide RNA.</p>
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<p><img src = "https://static.igem.org/mediawiki/2015/c/cf/WMdCas9_Repression_Plot.png" width = 500></p>
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<p>The breadth of the variety in our part collection provides future teams with the flexibility required to actualize their network designs without inhibition from a lack of resources. </p>
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</p>
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Latest revision as of 04:43, 21 November 2015

NOISE - W&M iGEM

Part Collection

In keeping with our theme of promoter effects on transcription, we created a suite of guide RNAs for the targeted dCas9 repression of 11 of the most frequently used promoters in the Registry. For cells which express a dCas9 protein, the transformation of any of our guide RNA parts will repress the expression of any protein under the associated promoter. We also included a scrambled guide RNA to serve as a negative control for troubleshooting purposes.

(BELOW) An example of repression: fluorescence values for RFP under the R0010 promoter, in cells with and without the R0010 guide RNA.

The breadth of the variety in our part collection provides future teams with the flexibility required to actualize their network designs without inhibition from a lack of resources.