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| | </ul> | | </ul> |
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| − | <p>The bacterial strains containing Shewanella Mtr electron transport system used in this work were kindly provided by the laboratory of <strong>Carolyn Ajo Franklin</strong></a> (Berkeley National laboratory)</p> | + | <p>The bacterial strains containing Shewanella Mtr electron transport system used in this work were kindly provided by the laboratory of <strong>Carolyn Ajo Franklin</strong></a> (Berkeley National laboratory). <strong>Giuliano Berloffa</strong> from the Mansy lab for helping with some good tips on protein purification and for troubleshooting with the anaerobic chamber</p> |
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| | </div><br />Microbial Fuel Cell (MFC) </h4> | | </div><br />Microbial Fuel Cell (MFC) </h4> |
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| − | <p>The small MFCs used in this work were built by kindly provided by Martin Hanczyc (CIBIO, Univ. of Trento) and Ioannis Ieropoulos (UWE Bristol). The big MFC prototype was designed by <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_alessandro" class="authorCite">Alessandro</a> and <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_riccardo" class="authorCite">Riccardo</a> and assembled using parts of the small MFCs and parts built by the mechanic workshop of the Physics department of the University of Trento. Suresh Bonotto, Damiano Avi , Luca Matteo, Mariano and Giggi Minati provided important technical information for the design of the big MFC.</p> | + | <p>The small MFCs and some of the materials used in this work were kindly given to us by <strong>Martin Hanczyc </strong> (CIBIO, Univ. of Trento) and built by Ioannis Ieropoulos (UWE Bristol). The big MFC prototype was designed by <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_alessandro" class="authorCite">Alessandro</a> and <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_riccardo" class="authorCite">Riccardo</a> and assembled using parts of the small MFCs and parts built by the mechanic workshop of the Physics department of the University of Trento. Suresh Bonotto, Damiano Avi, Mariano Dimauro and Gigi Minati provided important technical information for the design of the big MFC.</p> |
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| | <h4 class="header4 wow flipInX" style="text-align:center;"><div href="#" class="rotate-box square-icon" style="text-align:center;"> | | <h4 class="header4 wow flipInX" style="text-align:center;"><div href="#" class="rotate-box square-icon" style="text-align:center;"> |
| | <span class="rotate-box-icon"><i class="faa flaticon-bars-graphic"></i></span> | | <span class="rotate-box-icon"><i class="faa flaticon-bars-graphic"></i></span> |
| − | </div><br />Intrelab Study</h4> | + | </div><br />Interlab Study</h4> |
| | <p> | | <p> |
| − | <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_elisa" class="authorCite">Elisa</a> and <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_claudio" class="authorCite">Claudio</a>. Fabio Chizzolini, Roberta Lentini and Noel Martin from the Sheref Mansy Lab helped in the experimental set up of FACS and realtime PCR. | + | <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_elisa" class="authorCite">Elisa</a> and <a href="https://2015.igem.org/Team:UNITN-Trento/Team#team_claudio" class="authorCite">Claudio</a>. Fabio Chizzolini, Roberta Lentini and Noel Martin from the Mansy Lab helped in the experimental set up of FACS and realtime PCR. Michele Forlin from the Mansy lab helped in generating the Heatmaps to analyze the data. |
| | </p> | | </p> |
| | </div> | | </div> |
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| | </div> | | </div> |
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| − | <p>During this summer we received support and help from our great Instructors: Cristina Del Bianco, Sheref Mansy, and Olivier Jousson. Rossella Tomazzolli, Margherita Kos and Matteo Grott allowed us to work all summer in a safe, clean and well equipped laboratory.</p> | + | <p>During this summer we received support and help from our great Instructors: <strong>Cristina Del Bianco</strong>, <strong>Sheref Mansy</strong>, <strong>Olivier Jousson</strong> and <strong>Martin Hanczyc</strong>. Rossella Tomazzolli, Margherita Kos, and Matteo Grott allowed us to work all summer in a safe, clean and well equipped laboratory.</p> |
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| | </div> | | </div> |
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| | <h3 class="wow fadeInDown">Acknowledgments</h3> | | <h3 class="wow fadeInDown">Acknowledgments</h3> |
| | </header> | | </header> |
| − | <p>We wish to thank the <a href="http://web.unitn.it/en/cibio" class="authorCite" target="_blank">Centre for Integrative Biology</a> (CIBIO) and the <a href="http://www.unitn.it/en" class="authorCite" target="_blank">University of Trento</a> for supporting our project and team. At the beginning of the summer we discussed details of our project with <strong>Paola Foladori</strong> from the Sanitary Engineering group of the Civil, Environmental and Mechanical Engineering department, which is an expert in MFC and water depuration. Thank you for your suggestions Paola!</p> | + | <p>We wish to thank the <a href="http://web.unitn.it/en/cibio" class="authorCite" target="_blank">Centre for Integrative Biology</a> (CIBIO) and the <a href="http://www.unitn.it/en" class="authorCite" target="_blank">University of Trento</a> for supporting our project and team. |
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| − | <p>We wish to thank Prof. <strong>Graziano Guella</strong>, who helped us with some chemical issues for carotenoids/retinoids extraction and <strong>Luca Matteo</strong> from the physics department of UniTN for a crash course in electrical engineering. Additional thanks to Tu-Delft for sending us the plasmids of Mtr and the <strong>Guido Grandi Lab</strong> at CIBIO (Trento) for providing us the genome of BL21. Giuliano Berloffa from the Mansy lab for helping with some good tips on protein purification and for troubleshooting with the anaerobic chamber.</p> | + | <p>We wish to thank Prof. <strong>Graziano Guella</strong>, who helped us with some chemical issues for carotenoids/retinoids extraction and <strong>Luca Matteo</strong> from the physics department of UniTN for a crash course in electrical engineering. Additional thanks to Tu-Delft for sending us the plasmids of Mtr and the <strong>Guido Grandi Lab</strong> at CIBIO (Trento) for providing us the genome of BL21. <strong>Alice Spigo</strong> drawed the blueprint of the MFC. |
| − |
| + | |
| − |
| + | At the beginning of the summer we discussed details of our project with <strong>Paola Foladori</strong> from the Civil, Environmental and Mechanical Engineering department, which is an expert in MFC and water depuration. Thank you for your suggestions Paola! We also had a very informative chat with <strong>Michele Del Fabro</strong> on domestic energy waste.</p> |
| − | We used icons made by <a href="http://www.flaticon.com/authors/freepik" class="authorCite" target="_blank">Freepik</a><a href="http://cdn.flaticon.com/license/license.pdf" class="licenced custom" target="_blank"></a>, <a href="http://www.flaticon.com/authors/simpleicon" class="authorCite" target="_blank">Simpleicon</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a>, <a href="http://www.flaticon.com/authors/situ-herrera" class="authorCite" target="_blank">Situ Herrera</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a>, <a href="http://www.flaticon.com/authors/google" class="authorCite" target="_blank">Google</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a> and <a href="http://www.flaticon.com/authors/situ-herrera" class="authorCite" target="_blank">Austin Andrews</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a> from <a href="http://www.flaticon.com/" class="authorCite" target="_blank">FlatIcon</a>, and the <a href="http://fontawesome.io" class="authorCite" target="_blank">Font Awesome</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a>, package by Dave Gandy. | + | |
| | + | Last but not least we would like to thank our dear friend <strong>Damiano Avi</strong>, who always finds a way to help the iGEM teams here in Trento!</p> |
| | + | |
| | + | We used icons made by <a href="http://www.flaticon.com/authors/freepik" class="authorCite" target="_blank">Freepik</a><a href="http://cdn.flaticon.com/license/license.pdf" class="licenced custom" target="_blank"></a>, <a href="http://www.flaticon.com/authors/simpleicon" class="authorCite" target="_blank">Simpleicon</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a>, <a href="http://www.flaticon.com/authors/situ-herrera" class="authorCite" target="_blank">Situ Herrera</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a>, <a href="http://www.flaticon.com/authors/google" class="authorCite" target="_blank">Google</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a> and <a href="http://www.flaticon.com/authors/situ-herrera" class="authorCite" target="_blank">Austin Andrews</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a> from <a href="http://www.flaticon.com/" class="authorCite" target="_blank">FlatIcon</a>, and the <a href="http://fontawesome.io" class="authorCite" target="_blank">Font Awesome</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a>, package by Dave Gandy. |
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| − | This wiki page has been customized startin from the wounderful Twenty theme from <a href="http://html5up.net/twenty" class="authorCite" target="_blank">@n33co/HTML5UP</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a> using | + | This wiki page has been customized starting from the wounderful Twenty theme from <a href="http://html5up.net/twenty" class="authorCite" target="_blank">@n33co/HTML5UP</a><a href="http://creativecommons.org/licenses/by/3.0/" class="licenced CC" title="Released under the Creative Commons Attribution Licence" target="_blank"></a> using |
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| | </div> | | </div> |