Difference between revisions of "Team:UC Davis/Parts"

 
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<td align="center"><a href="https://2015.igem.org/Team:UC_Davis"><img src="https://static.igem.org/mediawiki/2015/5/5c/Banner_Final.png" width="907px" height="420" align = "top"></a></td>   
 
<td align="center"><a href="https://2015.igem.org/Team:UC_Davis"><img src="https://static.igem.org/mediawiki/2015/5/5c/Banner_Final.png" width="907px" height="420" align = "top"></a></td>   
  
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<a href="https://2015.igem.org/Team:UC_Davis/Project" style="text-decoration:none;color:#FFFFFF">PROJECT</a> </td>
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<a href="https://2015.igem.org/Team:UC_Davis/Parts" style="text-decoration:none;color:#FFFFFF">ACHIEVEMENTS </a> </td>
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<a href="https://2015.igem.org/Team:UC_Davis/Design" style="text-decoration:none;color:#FFFFFF">PROJECT</a> </td>
  
 
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<a href="https://2015.igem.org/Team:UC_Davis/Attributions" style="text-decoration:none;color:#FFFFFF">ATTRIBUTIONS </a> </td>
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<a href="https://2015.igem.org/Team:UC_Davis/Parts" style="text-decoration:none;color:#FFFFFF">ACHIEVEMENTS</a> </td>
  
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<a href="https://2015.igem.org/Team:UC_Davis/Attributions" style="text-decoration:none;color:#FFFFFF">THE TEAM</a> </td>
  
 
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<a href="https://2015.igem.org/Team:UC_Davis/Safety" style="text-decoration:none;color:#FFFFFF">SAFETY & PROTOCOLS </a></td>
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<a href="https://2015.igem.org/Team:UC_Davis/Safety" style="text-decoration:none;color:#FFFFFF">SAFETY & PROTOCOLS</a></td>
  
 
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<br><img src="https://static.igem.org/mediawiki/2015/5/52/Achievements_UCD.png" width="981px" height="65"></a>                      <!--CHANGE OUT HEADER HERE-->
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<img src="https://static.igem.org/mediawiki/2015/5/52/Achievements_UCD.png" width="981px" height="65"></a>                      <!--CHANGE OUT HEADER HERE-->
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<br>
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<a href="#results"> 1. Results<br>
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<p align = "left"><font size="3" face = "Avenir" >  
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<a href="#parts"> 2. Parts<br>
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<a href="#medalreq"> 3. Medal Requirements<br><br><br>
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Table of Contents:<br><br>
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<a href="#parts"> 1. BioBricks<br><br>
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<a href="#medalreq"> 2. Medal Requirements<br>
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<a href="#menu" style="text-decoration:none" color="#FFFFFF" fillerwordsyeeeeeah</font><font color = "#6A181B" font size="11">&nbsp; &nbsp; &nbsp; &nbsp; &#x21a5;</font></a>
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                                                                                                                                                                                                             <!-------------- PARTS------------->
 
                                                                                                                                                                                                             <!-------------- PARTS------------->
 
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<img src="https://static.igem.org/mediawiki/2015/7/7c/Attributions_2_UCD.png" width="983px" height="100"></a><br><br>                          <!--CHANGE OUT BANNER HERE-->
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<img src="https://static.igem.org/mediawiki/2015/0/00/BiobricksUCD2015.png" width="983px" height="100"></a><br><br>                          <!--CHANGE OUT BANNER HERE-->
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<font size = "3" face = "Avenir">Click through for part registry!</font><br><br>
  
 
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<img src="https://static.igem.org/mediawiki/2015/4/49/Bba_K171200_UCD.png" align = "left" width="241px" height="177" >
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<a href = "http://parts.igem.org/Part:BBa_K1712000">
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<img src="https://static.igem.org/mediawiki/2015/4/49/Bba_K171200_UCD.png" align = "left" width="241px" height="177" ></a>
 
<font color= "white"> this is random crap  </font>
 
<font color= "white"> this is random crap  </font>
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<img src="https://static.igem.org/mediawiki/2015/6/62/Bba_K1712002_UCD.png"  align = "middle" width="241px" height="177" >  
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<a href = "http://parts.igem.org/Part:BBa_K1712002">
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<img src="https://static.igem.org/mediawiki/2015/e/e6/Bba_K1712001_UCD.png" align = "right" width="241px" height="177">  
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<img src="https://static.igem.org/mediawiki/2015/6/62/Bba_K1712002_UCD.png"  align = "middle" width="241px" height="177" > </a>
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<a href = "http://parts.igem.org/Part:BBa_K1712001">
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<img src="https://static.igem.org/mediawiki/2015/e/e6/Bba_K1712001_UCD.png" align = "right" width="241px" height="177"> </a>
 
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<font color= "white"> this is random crap don't de</font>
 
<font color= "white"> this is random crap don't de</font>
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  <img src="https://static.igem.org/mediawiki/2015/5/5b/Bba_K1712003_UCD.png" align = "center" width="241px" height="177" >
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<a href = "http://parts.igem.org/Part:BBa_K1712003">
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  <img src="https://static.igem.org/mediawiki/2015/5/5b/Bba_K1712003_UCD.png" align = "center" width="241px" height="177" ></a>
 
<font color= "white"> this is random crap please </font>  
 
<font color= "white"> this is random crap please </font>  
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<img src="https://static.igem.org/mediawiki/2015/6/6e/Bba_K1712004_UCD.png" align = "center" width="241px" height="177" >
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<a href = "http://parts.igem.org/Part:BBa_K1712004">
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<img src="https://static.igem.org/mediawiki/2015/6/6e/Bba_K1712004_UCD.png" align = "center" width="241px" height="177" ></a>
 
</p>                                                                                                                                                                                                                            <!--PUT CONTENT HERE-->
 
</p>                                                                                                                                                                                                                            <!--PUT CONTENT HERE-->
  
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<a href="#menu" style="text-decoration:none" color="#FFFFFF" fillerwordsyeeeeeah</font><font color = "#6A181B" font size="11">&nbsp; &nbsp; &nbsp; &nbsp; &#x21a5;</font></a>
 
<a href="#menu" style="text-decoration:none" color="#FFFFFF" fillerwordsyeeeeeah</font><font color = "#6A181B" font size="11">&nbsp; &nbsp; &nbsp; &nbsp; &#x21a5;</font></a>
 
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<img src="https://static.igem.org/mediawiki/2015/3/37/Attributions_3_UCD.png" width="983px" height="100"></a><br><br>                         <!--CHANGE OUT BANNER HERE-->
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<img src="https://static.igem.org/mediawiki/2015/c/c4/MedalRequirementsUCD2015.png" width="983px" height="100"></a><br><br>                        
  
 
                                                                                                                                                                                                                                   <!--PUT CONTENT HERE-->
 
                                                                                                                                                                                                                                   <!--PUT CONTENT HERE-->
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<p class ="big"> <font size="5" face = "Avenir">Bronze Medal</font></p>
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<font size = "3" face = "Avenir">
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Team <a href ="https://igem.org/Team.cgi?id=1712">registration</a><br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Completed <a href ="https://igem.org/2015_Judging_Form?id=1712">judging form</a><br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Team Wiki<br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Presented poster and talk at iGEM Jamboree<br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left><a href ="https://2015.igem.org/Team:UC_Davis/Attributions">Attribute</a> work done by students<br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Submitted 5 new parts to the registry: <a href ="http://parts.igem.org/Part:BBa_K1712000">Bba_K1712000</a>, <a href ="http://parts.igem.org/Part:BBa_K1712001">Bba_K1712001</a>, <a href ="http://parts.igem.org/Part:BBa_K1712003">Bba_K1712003</a>, <a href ="http://parts.igem.org/Part:BBa_K1712004">Bba_K1712004</a>, <a href ="http://parts.igem.org/Part:BBa_K1712005">Bba_K1712005</a><br><br>
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<p class ="big"> <font size="5" face = "Avenir">Silver Medal</font></p>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Characterized and submitted 5 fabi genes (4 new, 1 old) to the registry<br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Submitted 5 parts in appropriate pSB1C3 vector<br><br>
  
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To satisfy Gold medal requirements, the 2015 UC Davis iGEM Team:<br>
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<p class ="big"> <font size="5" face = "Avenir">Gold Medal</font></p>
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<b>1. Demonstrated strong Human Practices</b> by integrating the investigated issue into the design/execution of our project:<br><br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>Our project was closely informed by our <a href="https://2015.igem.org/Team:UC_Davis/Practices">Policy & Practices</a><br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>We demonstrated a <a href="https://2015.igem.org/Team:UC_Davis/Project#WL4">functioning prototype</a> in real world (waste water) conditions <br>
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While we developed a biosensor for triclosan specifically, the overarching goal of our project is to push for more responsible chemical use at large. We realized if we wanted to address the issue of responsible chemical use, we had to go about the issue responsibly ourselves. This meant making a concerted effort to understand the complexities of the issue, learning from the past and present in crafting a solution for the future, and assessing the implications of our proposed solution.<br><br>
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<img src="https://static.igem.org/mediawiki/2015/5/5f/Achievements_Check2_UCD.png" width ="21.5626px" height = "18.75" align = left>We improved the function and characterization of <a href ="http://parts.igem.org/Part:BBa_K771303">BBa_K771303</a> by:<br>
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Because triclosan predominantly makes its way into the environment through waste-water effluent, we started exploring our problem space by better understanding how triclosan and other chemicals are regulated and monitored at the waste-water treatment plant level.<br>
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   <li> We spoke with Michael Fan, Senior environmental engineer, who has been operating the waste-water treatment facility on campus for the past 25 years</li>
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   <li>Codon optimizing</li>
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   <li> We toured Regional San, a local waste-water treatment facility in Sacramento</li>
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  <li>Characterizing its expression, activity, and triclosan inhibition, and </li>
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   <li>Adding useful information to provide biological relevance</li>
 
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From these conversations we learned how waste-water effluent is currently monitored.<br><br>
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Our part: <a href ="http://parts.igem.org/Part:BBa_K1712000">BBa_K17120003</a></p>
  
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But despite these regulations, we learned from researchers at The San Francisco Estuary Institute that triclosan, among other chemicals in our consumer products, is still released into the environment and these environmental levels are in fact harming aquatic organism health.<br><br>
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But until there is a regulatory mandated permissible level, there is little incentive for waste water treatment plants to monitor for a specific compound in addition to the tests already being conducted on waste-water effluent. In essence, “no legislation, no market” -- further highlighting the complexity/interconnectedness of the issue- that you can’t just expect one “node” in the network of players to solve the issue.<br><br>
 
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We realized that all these players are interconnected, but that some players, have a stronger ability to push for change (ie. legislation) <br><br>
 
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To understand how we might be able to apply pressure to another node in the network we:<br><br>
 
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Read up on famous chemical regulation case studies to see what we might learn from the past:<br>
 
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<ul>
 
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  <li> From the DDT case study, we __ the influential role citizen scientist Rachel Carson played in raising public awareness and pushing for legislative action.</li>
 
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  <li> From the Chromium 6 case study, we ___ the role Erin Brockovich played in calling attention to the _____.</li>
 
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We then reached out to experts to figure out what is and needs to be done presently to address the issue:<br>
 
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  <li>From Arlene Blum, director of Green Science Policy Institute, we learned about  regulating chemicals by class instead of on an individual basis</li>
 
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  <li>From Bruce Hammock, _____, and Jonathon Eisen we learned ___ the need to justify the use of chemicals in products (benefits outweigh risks)</li>
 
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From this exploration, we identified <b>public awareness</b> as an effective catalyst for change. We researched civic engagement measures and identified why these initiatives were so effective in inspiring change. We gathered that the key is in fostering a sense of individual and collective responsibility and in providing a sense of agency thats grounded in reality.<br><br>
 
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We thought about how we might raise public awareness: who would our target audience be? How would we communicate our message? We were particularly inspired by Greg Neimeyer’s Black Cloud initiative, and identified schools as a likely candidate because:<br>
 
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  <li>we believe an important component of an education ought to be <b>learning about ones impact on the environment</b></li>
 
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  <li>and because school is where students learn to <b>think critically</b> and the complex issue of chemical regulation is an appropriate primer for the sorts of real world problems students will be grappling with later on in their careers</li>
 
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If we could fit our lesson plan to next generation standards, schools could be a viable way to get our message out into the public realm. We reached out to Community Resources for Science (CRS), an organization that works with scientists to bring their work into the classroom, for help in developing a lesson plan. Once we got the approval that our lesson plan met their standards, CRS connected us with their network of teachers in Davis.<br><br>
 
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We then took a step back to assess the possible implications of our triclosan biosensor and lesson plan. We harkened back to Bruce Hammock’s warning about playing into consumer fears and what Arlene Blum told us about the futile cycle of replacing one toxic chemical with another. Cognizant of these two points, we developed an “antimicrobial awareness app” [MUNTAHA BLURB something to the effecct of “we wanted to inculcate responsible chemical use, not fear”]<br><br>
 
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Latest revision as of 01:27, 21 December 2015



Click through for part registry!

this is random crap

this is random crap don't de this is random crap please

        ↥


Bronze Medal

Team registration
Completed judging form
Team Wiki
Presented poster and talk at iGEM Jamboree
Attribute work done by students
Submitted 5 new parts to the registry: Bba_K1712000, Bba_K1712001, Bba_K1712003, Bba_K1712004, Bba_K1712005

Silver Medal

Characterized and submitted 5 fabi genes (4 new, 1 old) to the registry
Submitted 5 parts in appropriate pSB1C3 vector

Gold Medal

Our project was closely informed by our Policy & Practices
We demonstrated a functioning prototype in real world (waste water) conditions
We improved the function and characterization of BBa_K771303 by:
  • Codon optimizing
  • Characterizing its expression, activity, and triclosan inhibition, and
  • Adding useful information to provide biological relevance
Our part: BBa_K17120003
        ↥