Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Miscellaneous"
| Line 193: | Line 193: | ||
<li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | <li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | ||
<ul><li> in 5 ml LB-Medium (37°C)</li></ul> | <ul><li> in 5 ml LB-Medium (37°C)</li></ul> | ||
| − | <li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013) | + | <li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)</li> |
<ul><li> Plasmids stored at -20°C</li></ul> | <ul><li> Plasmids stored at -20°C</li></ul> | ||
<li>Second heat shock transformation of chem. competent KRX with BBa_I13504</li> | <li>Second heat shock transformation of chem. competent KRX with BBa_I13504</li> | ||
Revision as of 16:59, 21 July 2015
Miscellaneous
This Week we started with the iGEM 2015 Measurement Interlab Study:
- Heat shock transformation of BioBricks from the distribution:
- BBa_K823005 (Anderson promoter J23101)
- BBa_K823008 (Anderson promoter J23106)
- BBa_K823013 (Anderson promoter J23117)
- BBa_I13504 (GFP)
iGEM 2015 Measurement Interlab Study:
- Colonies from the BioBrick Transformation were used to inoculate over night cultures
- in 5 ml LB-Medium (37°C)
- Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)
- Plasmids stored at -20°C
- Second heat shock transformation of chem. competent KRX with BBa_I13504
- Colonies were used to inoculate over night cultures (5 ml LB, 37°C)