Difference between revisions of "Team:San Andres/Parts"

Line 6: Line 6:
 
</head>
 
</head>
 
<body>
 
<body>
 +
<meta content="text/html; charset=ISO-8859-1"
 +
http-equiv="content-type">
 +
<title></title>
 
<title>exp</title>
 
<title>exp</title>
 
<meta http-equiv="Content-Type"
 
<meta http-equiv="Content-Type"
Line 554: Line 557:
 
       </td>
 
       </td>
 
       <br>
 
       <br>
−
      </td>
 
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>
Line 616: Line 618:
 
   <li><big><span
 
   <li><big><span
 
  style="font-family: 'Arial','sans-serif';"><strong
 
  style="font-family: 'Arial','sans-serif';"><strong
−
  style="font-weight: bold;"><a
+
  style="font-weight: bold;">Promoter
−
href="http://parts.igem.org/Part:BBa_J23119">Promoter</a>
+
    <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
−
(BBa_J23119</strong><span style="font-weight: bold;">)</span>:
+
href="http://parts.igem.org/Part:BBa_J23119"><span
 +
style="font-weight: bold;">)</span></a>:
 
     </span>Constitutive
 
     </span>Constitutive
 
promoter (which works permanently) that is give in the relative
 
promoter (which works permanently) that is give in the relative
 
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li>
 
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li>
−
   <li><big><strong style="font-weight: bold;"><a
+
   <li><big><strong style="font-weight: bold;">RBS</strong><strong
−
  href="http://parts.igem.org/Part:BBa_K1084103">RBS</a>
+
  style="font-weight: bold;">
−
(BBa_K1084103)</strong>: Synthetic RBS with uplifting sequence.</big></li>
+
    <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 +
Synthetic RBS with uplifting sequence</big>.</li>
 +
  <li><big><strong>Vector:<span
 +
style="font-weight: normal;"> <a
 +
href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big></li>
 
   <li><big><strong><span
 
   <li><big><strong><span
−
  style="font-family: 'Arial','sans-serif';"><a
+
  style="font-family: 'Arial','sans-serif';">Coding Region:
−
href="http://parts.igem.org/Part:pSB1C3">Vector:</a><span
+
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
−
style="font-weight: normal;"> pSB1C3</span></span></strong></big></li>
+
It
−
  <li><big><strong><span
+
−
style="font-family: 'Arial','sans-serif';"><a
+
−
href="http://parts.igem.org/Part:BBa_K590087">Coding Region:
+
−
KumaMax</a> (BBa_K590087):</span></strong> It
+
 
degrades gluten,
 
degrades gluten,
 
celiac disease leading cause. Enzyme generated by rational mutation for
 
celiac disease leading cause. Enzyme generated by rational mutation for
Line 639: Line 642:
 
2011.</a></big></li>
 
2011.</a></big></li>
 
   <li><big><strong><span
 
   <li><big><strong><span
−
  style="font-family: 'Arial','sans-serif';"><a
+
  style="font-family: 'Arial','sans-serif';">Reporter: RFP
−
href="http://parts.igem.org/Part:BBa_J04450">Reporter: RFP</a>
+
    <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span
−
(BBa_J04450):</span></strong><span
+
 
  style="font-family: Arial,sans-serif;"> Red f</span>luorescence
 
  style="font-family: Arial,sans-serif;"> Red f</span>luorescence
 
protein.</big></li>
 
protein.</big></li>
−
   <li><big><span style="font-family: Arial,sans-serif;"><strong><a
+
   <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
−
href="http://parts.igem.org/Part:BBa_B0015">Terminator</a>
+
    <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
−
(BBa_B0015):</strong> </span>Dual terminator consisting of
+
    </span>Dual terminator consisting of
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
transcription</big>.</li>
 
transcription</big>.</li>
Line 676: Line 678:
 
  alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
 
  alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
 
  class="gwd-img-kwqf"></a>
 
  class="gwd-img-kwqf"></a>
 +
<br>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 20:40, 24 July 2015

exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg


Home Team Notebook Project Parts
Modeling Results Human Practices Future Projections Collaborations

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:1.png
File:Circuito.jpg
This is a graphic model of as it has be our plasmid where we can visualize the promoter, the RBS, the enzyme KumaMax, the RFP and the terminator, joined by means of prefixes and suffixes that indicate the locations of court.

File:Plasmido.jpg