Difference between revisions of "Team:UCL/Experiments"
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<div id="ligation"><h2>Ligation protocol</h2> | <div id="ligation"><h2>Ligation protocol</h2> | ||
<ol> | <ol> | ||
− | <li> Calculate the amount of insert DNA required to maintain 1:3 backbone:insert molar ratio. | + | <li> Calculate the amount of insert DNA required to maintain 1:3 backbone:insert molar ratio using formula below. For standard ligations use 50 ng of vector DNA, increase the amounts of DNA if unsuccessful.<br/> |
− | <img src="https://static.igem.org/mediawiki/2015/9/9a/UCL_ligationformula.png.png"> | + | <img src="https://static.igem.org/mediawiki/2015/9/9a/UCL_ligationformula.png.png">. </li> |
<li> Prepare the ligation mixture: | <li> Prepare the ligation mixture: | ||
<ul> - required amount of insert DNA</ul> | <ul> - required amount of insert DNA</ul> |
Revision as of 16:07, 5 August 2015