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| − | <h5>Experiments & Protocols</h5>
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| − | <p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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| − | <ul>
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| − | <li> Protocols </li>
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| − | <li> Experiments </li>
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| − | <li>Documentation of the development of your project </li>
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| − | <div id="transformation"><h2>Transformation protocol </h2> | + | |
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| − | <li>(If using part from the distribution: resuspend the DNA in 10 ul of MiliQ water, making sure that it turns red. Wait 10 minutes before adding the DNA to cells)</li>
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| − | 2. Put a tube of NEB DH 5 alpha <i>E. coli</i> cells on ice and wait until they thaw completely. Divide the cells into 50 ul aliquotes. <br/>
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| − | 3. Add 1 ul of plasmid DNA to 50 ul of cells. <br/>
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| − | 4. Mix by carefully flicking the tube. Do not vortex or pipette in and out! <br/>
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| − | 5. Place the mixture on ice for 30 minutes. <br/>
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| − | 6. Heat shock the cells at 42 °C for 30 seconds and immediately put on back on ice.<br/>
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| − | 7. Keep cells on ice for next 5 minutes. Do not mix. <br/>
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| − | 8. Pipette 950 ul of SOC (or LB) media kept at room temperature into the mixture. <br/>
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| − | 9. Incubate the mixture at 37 °C for 60 minutes <br/>
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| − | 10. Plate 50 ul of cells to the plate with appropriate antibiotic and incubate overnight at 37 °C
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