Difference between revisions of "Team:Dundee/labjournal manu"

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             <span class="box-title">Day 2</span>
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                   <p><b>Aim of experiment:</b> ultricies nec tellus eu</p>
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                   <p>Protocols Used:
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                  <p class="journal-content">faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.</p>
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                   <p><b>Aim of experiment:</b> To prepare <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> for sequence confirmation, storage, and further processing.</p>
 +
 
 +
                   <p><b>Protocols Used:</b>
 +
                    <p><a data-toggle="modal" data-target="#plating-modal" class="journal-protocol"> Click here to see our plating protocol.</a></p>
 +
                    <p><a data-toggle="modal" data-target="#overnightculture-modal" class="journal-protocol"> Click here to see our overnight culture protocol.</a></p></p>
 +
 
 +
                  <p><b>Results:</b> N/A</p>
 +
 
 +
                  <!-- For images in the results use the following line of code:
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 +
                  <p><b>Results:</b><a data-toggle="modal" data-target="#lbpa-figure1-modal" class="journal-protocol"> Figure 1 </a></p>
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 +
                  under data-target refer to a modal specifically made for each image under image modals section-->
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 +
 
 +
                  <p><b>Next Steps:</b> Purification of the Plasmid, containing <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> and confirmation of size and sequence.</p>
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                   <p><b>Aim of experiment:</b>Storage of BBa_K1058008</p>
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                  <p class="journal-content">The agar stab received from the registry was transferred to an agar plate supplemented with 2x Chloramphenicol. The plate was incubated at 37°C for 16h and then stored at 4°C. Furthermore, an overnight culture was set up by inoculating 5ml LB, supplemented with chloramphenicol, with the agar stab. The overnight culture was incubated for 16h. </p>
+
                   <p><b>Aim of experiment:</b> To prepare <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> for sequence confirmation, storage, and further processing.</p>
 +
 
 +
                   <p><b>Protocols Used:</b>
 +
                    <p><a data-toggle="modal" data-target="#plating-modal" class="journal-protocol"> Click here to see our plating protocol.</a></p>
 +
                    <p><a data-toggle="modal" data-target="#overnightculture-modal" class="journal-protocol"> Click here to see our overnight culture protocol.</a></p></p>
 +
 
 +
                  <p><b>Results:</b> N/A</p>
 +
 
 +
                  <!-- For images in the results use the following line of code:
 +
 
 +
                  <p><b>Results:</b><a data-toggle="modal" data-target="#lbpa-figure1-modal" class="journal-protocol"> Figure 1 </a></p>
 +
 
 +
                  under data-target refer to a modal specifically made for each image under image modals section-->
 +
 
 +
 
 +
                  <p><b>Next Steps:</b> Purification of the Plasmid, containing <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> and confirmation of size and sequence.</p>
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                   <p><b>Aim of experiment:</b>Purification and sequencing of BBa_K1058008</p>
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                  <p class="journal-content">The overnight culture was retrieved and centrifuged at 4000rpm for 10min. The plasmid, pSB1C3, containing BBa_K1058008 was purified according to the miniprep protocol. The concentration was determined by nanodrop and found to be 190.9ng/µl.
+
                   <p><b>Aim of experiment:</b> To prepare <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> for sequence confirmation, storage, and further processing.</p>
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                  2 Samples were submitted for sequencing, each containing 550ng DNA, 2µl of 3mM primer, and water filled up to a total volume of 30µl</p>
+
 
 +
                   <p><b>Protocols Used:</b>
 +
                    <p><a data-toggle="modal" data-target="#plating-modal" class="journal-protocol"> Click here to see our plating protocol.</a></p>
 +
                    <p><a data-toggle="modal" data-target="#overnightculture-modal" class="journal-protocol"> Click here to see our overnight culture protocol.</a></p></p>
 +
 
 +
                  <p><b>Results:</b> N/A</p>
 +
 
 +
                  <!-- For images in the results use the following line of code:
 +
 
 +
                  <p><b>Results:</b><a data-toggle="modal" data-target="#lbpa-figure1-modal" class="journal-protocol"> Figure 1 </a></p>
 +
 
 +
                  under data-target refer to a modal specifically made for each image under image modals section-->
 +
 
 +
 
 +
                  <p><b>Next Steps:</b> Purification of the Plasmid, containing <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> and confirmation of size and sequence.</p>
 +
                 
 
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                  <p><b>Aim of experiment:</b> To prepare <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> for sequence confirmation, storage, and further processing.</p>
 +
 
 +
                  <p><b>Protocols Used:</b>
 +
                    <p><a data-toggle="modal" data-target="#plating-modal" class="journal-protocol"> Click here to see our plating protocol.</a></p>
 +
                    <p><a data-toggle="modal" data-target="#overnightculture-modal" class="journal-protocol"> Click here to see our overnight culture protocol.</a></p></p>
 +
 
 +
                  <p><b>Results:</b> N/A</p>
 +
 
 +
                  <!-- For images in the results use the following line of code:
 +
 
 +
                  <p><b>Results:</b><a data-toggle="modal" data-target="#lbpa-figure1-modal" class="journal-protocol"> Figure 1 </a></p>
 +
 
 +
                  under data-target refer to a modal specifically made for each image under image modals section-->
 +
 
 +
 
 +
                  <p><b>Next Steps:</b> Purification of the Plasmid, containing <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> and confirmation of size and sequence.</p>
 +
                 
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+
                   <p><b>Aim of experiment:</b> To prepare <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> for sequence confirmation, storage, and further processing.</p>
 +
 
 +
                   <p><b>Protocols Used:</b>
 +
                    <p><a data-toggle="modal" data-target="#plating-modal" class="journal-protocol"> Click here to see our plating protocol.</a></p>
 +
                    <p><a data-toggle="modal" data-target="#overnightculture-modal" class="journal-protocol"> Click here to see our overnight culture protocol.</a></p></p>
 +
 
 +
                  <p><b>Results:</b> N/A</p>
 +
 
 +
                  <!-- For images in the results use the following line of code:
 +
 
 +
                  <p><b>Results:</b><a data-toggle="modal" data-target="#lbpa-figure1-modal" class="journal-protocol"> Figure 1 </a></p>
 +
 
 +
                  under data-target refer to a modal specifically made for each image under image modals section-->
 +
 
 +
 
 +
                  <p><b>Next Steps:</b> Purification of the Plasmid, containing <a href="http://parts.igem.org/Part:BBa_K1058008">BBa_K1058008</a> and confirmation of size and sequence.</p>
 +
                 
 
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Revision as of 18:46, 6 August 2015

LABJOURNAL

BioSpray

Our forensic toolkit aims to use synthetic biology approaches to improve on the current methods used by crime scene investigators.

Chromium Detector

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur a tincidunt elit. Aliquam porta nibh at enim luctus, auctor consequat dolor vehicula.

Fingerprint Aging

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur a tincidunt elit. Aliquam porta nibh at enim luctus, auctor consequat dolor vehicula.

Week Beginning 22/06

Summary

This week was all about getting started with the newly arrived biobrick BBa_K1058008

Day 1

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Day 2

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Day 3

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Day 4

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Day 5

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Day 6

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Day 7

Aim of experiment: To prepare BBa_K1058008 for sequence confirmation, storage, and further processing.

Protocols Used:

Click here to see our plating protocol.

Click here to see our overnight culture protocol.

Results: N/A

Next Steps: Purification of the Plasmid, containing BBa_K1058008 and confirmation of size and sequence.

Week 2

Week summary

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 1

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 2

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 3

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 4

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.

Day 5

Aim of experiment: ultricies nec tellus eu

Protocols Used:

faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl. Morbi felis lectus, ultricies nec tellus eu, faucibus eleifend lectus. In molestie augue leo, id imperdiet ante imperdiet at. Quisque ultrices neque sit amet felis tincidunt tristique at sed nisi. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Ut suscipit ut massa in ultricies. Nunc nec tincidunt nunc, ut consectetur lorem. Sed non justo nislo nislo nisl.