Difference between revisions of "Team:UNITN-Trento/Test"

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<h2>UNITN iGEM 2015</h2>
 
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<p>Electron-production with <br /> engineered <i>E. coli</i> and <br /> <strong>proteorhodopsin</strong></p>
 
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<h2>Electron production with <strong><i>E. coli</i></strong> and proteorhodopsin </h2>
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<h2><strong>Notebook</strong></h2>
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<p>We have done many things...</p>
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<p>The aim of our project is to develop a pseudoautotrophic <span class="i_enph">E. coli</span>, enabling it to live in the microbial fuel cell anaerobic condition. Our idea is to engineer E. coli with proteorhodopsin, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with blh, that encodes for the enzyme that cleaves beta-carotene and produces retinal.</p>
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<p>To increase electron production we plan to overexpress pncB that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production. Our bacterial device will give rise to a self-sustainable system, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.</p>
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<li><a href="#" id="cd-timeline_PR_btn" class="button small PR" onclick="javascript:scrollandfade('cd-timeline_PR')">Proteorhodopsin</a></li>
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<li><a href="#" id="cd-timeline_BLH_btn" class="button small BLH" onclick="javascript:scrollandfade('cd-timeline_BLH')">β-carotene<br/>dioxygenase</a></li>
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<p class="pintest">Electricity from E. coli<br/>
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<span>Check out our amazing idea! Have fun!<br/>
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<p class="pintest">Electricity from E. coli<br/>
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<span>Check out our amazing idea! Have fun!<br/>
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<p class="pintest">Electricity from E. coli<br/>
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<span>Check out our amazing idea! Have fun!<br/>
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<p class="pintest">Meet the team!<br/>
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<span>We are UNITN iGEM 2015 and we are such essence as dNTPs are made on<br/>
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<a class="btn" href="#"> To->Team </a></span></p>
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<p class="pintest">Catch 'em all!<br/>
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<span>Many techniques, different strains and so much fluorescence… Enjoy the Measurement Interlab Study<br/>
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<a class="btn" href="#"> To->Interlab </a></span></p>
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<p class="pintest">Synthetic Biology and mountains<br/>
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<span>Admire our lovely city, surrounded by mountains. Trento is waiting for you<br/>
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<a class="btn" href="#"> To->Interlab </a></span></p>
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<h2>Achievements</h2>
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<h4># new BioBricks</h4>
 
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<p>We built and submitted a total number of new BioBricks and characterized # of them. Check out our [Parts] page to know more!</p>
 
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<h4>Improved # Parts</h4>
 
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<h4>Measurements Interlab Study</h4>
 
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<p>We are participating also to the Measurements Interlab Study. Check out our progress in the Interlab Section!</p>
 
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Revision as of 19:38, 9 August 2015

UNITN-TRENTO iGEM 2015

Notebook

We have done many things...