Difference between revisions of "Team:UNITN-Trento/Test"

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<header>
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<h2><strong>Meet the UNITN Team</strong></h2>
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<h2><strong>Work hard, Work safe</strong></h2>
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<p>Alessandro, Elisa, Claudio, Silvia, Veronica, Riccardo and Moreno</p>
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<p> ? </p>
 
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<h2> Team Members </h2>
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<section>
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<div class="teamEntry left">
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<header>
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<div class="team_image_container style1" style="background-image:url('https://static.igem.org/mediawiki/2015/a/a0/Unitn_pics_team_alessandro.jpg');"></div>
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<div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria3"></i></span></div>  
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<div class="team_content">
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</header>
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<p class="main_name">Alessandro Turcato</p>
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<p>We have reviewed our organisms and parts against the <a class="i_linker" href="https://2015.igem.org/Safety/WhiteList" target="_blank">White List</a>. All the parts and bacterial strains that we used are in the <span class="i_enph">Risk Group 1</span></p>
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</section>
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<p>Hi.. I’m Alessandro Turcato I’m from Marano Vicentino in Veneto (Italy). I’m attending the third year of "Biomolecular sciences and technologies" B.Sc. in Trento. I really enjoy playing guitar and playing football furthermore I’m very happy to join the IGEM competition. I’m mainly involved into MFC part of our project and into envelope our own prototype. I hope you guys enjoy our work… see you in Boston!!</p>
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<p class="skill_list"><span>Skills:</span> Biology, Safety</p>
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</div>
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</div>
 
</div>
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<div class="4u 12u(narrower)">
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<div style="clear:both;"></div>
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<div class="teamEntry right">
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<header>  
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<div class="team_image_container style2" style="background-image:url(' ');"></div>
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<div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria3"></i></span></div>  
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<div class="team_content">
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</header>
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<p class="main_name">Elisa Godino</p>
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<p>We have submitted to iGEM headquarters <span class="i_enph">“About Our Lab”</span> and <span class="i_enph">“About Our Project”</span> questionnaires by the June 26<sup>th</sup> deadline</p>
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</section>
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<p>Hi! I'm Elisa Godino, I'm 22 years old and I was born in Cles (TN Italy). I recently graduated in Biomolecular Sciences and Technology at the University of Trento. I love science and I'm having a great time with this incredible iGEM experience. I also like going to the lake with friends and play beach volley. Sometimes I like to make cakes to the delight of my teammates! Mainly I deal with the Measurement Interlab Study and with Human Practices.</p>
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<p class="skill_list"><span>Skills:</span> Biology, Safety</p>
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</div>
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</div>
 
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<div class="team_image_container style3" style="background-image:url('https://static.igem.org/mediawiki/2015/d/d4/Unitn_pics_team_claudio.jpg');"></div>
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<div href="#" class="rotate-box square-icon"><span class="rotate-box-icon"><i class="faa flaticon-bacteria3"></i></span></div>
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<div class="team_content">
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</header>
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<p class="main_name">Claudio Oss Pegorar</p>
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<p>We have submitted to iGEM headquarters the <span class="i_enph">“Final Safety Form”</span> by the August 28<sup>th</sup> deadline</p>
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</section>
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</div>
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</div>
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</section>
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<!-- One -->
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 +
<!-- Content -->
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<div class="content">
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<section>
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<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>
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<header>
 +
<h3>About Safety</h3>
 +
</header>
 
 
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<p>Hi! I am Claudio Oss Pegorar, I am 22 and I was born in Trento (Italy). I am studying "Biomolecular sciences and technologies" as a B.Sc. student at the University of Trento. What I like to do other than staying in the lab is playing the guitar, day tripping and hanging out with my friends. I am grateful I had the chance to participate and contribute to this project and I enjoy every single day as an iGEMer. I am mainly involved into lab work, InterLab Study work and Safety.</p>
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<p>We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:</p>  
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<p class="skill_list"><span>Skills:</span> Biology, Safety</p>
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<ul>
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</div>
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<li>General considerations on risks and prevention</li>
 +
<li> Electrical Hazard</li>
 +
<li> Chemical Hazard</li>
 +
<li> Biological Hazard</li>
 +
<li> Compressed gasses and cryogenic liquids</li>
 +
</ul>
 +
<p>We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.</p>
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</section>
 
</div>
 
</div>
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<div style="clear:both;"></div>
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</section>
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<div class="teamEntry right">
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<div class="team_image_container style4" style="background-image:url('https://static.igem.org/mediawiki/2015/0/0d/Unitn_pics_team_silvia.jpg');"></div>
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<div class="team_content">
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<!-- Content -->
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<p class="main_name">Silvia Galvan</p>
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<div class="content">
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<section>
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<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>
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<header>
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<h3>Safe Project Design</h3>
 +
</header>
 +
<!-- TODO -->
 
 
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<p>I’m Silvia Galvan, I’m 22 years and I was born in Borgo Valsugana (Trento), where I live with my family. I am currently a student of the Bachelor of Science in Biomolecular Sciences and Technology at the University of Trento. My passion is running and I do Athletics since I was a child. I enjoy spending time with my two lovely nephews, and with my teammates of course! We’re having such a great summer in the lab, the work is hard sometimes but we have lots of fun together. I feel really lucky to have had the possibility to be an IGEMer and to have found such a great team! I am mainly dealing with the proteorhodopsin part.
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<div class="row">
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Fun fact: I have a dog named Brick, sometimes I call him BioBrick!</p>
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<div class="6u 12u(narrower)">
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<section>
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<header>
 +
<h4>Who will use your product? What opinions do these people have about your project?</h4>
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</header>
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<p>ANSWER</p>
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</section>
 +
</div>
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<div class="6u 12u(narrower)">
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<section>
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<header>
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<h4>Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?</h4>
 +
</header>
 +
<p>ANSWER</p>
 +
</section>
 +
</div>
 +
</div>
 +
<p>If your product is successful, who will receive benefits and who will be harmed?</p>
 +
<p>What happens when it's all used up? Will it be sterilized, discarded, or recycled?</p>
 +
<p>Is it safer, cheaper, or better than other technologies that do the same thing?</p>
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</section>
 +
</div>
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</section>
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<section class="wrapper style4 container" style="margin-top:1em;">
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 +
<!-- Content -->
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<div class="content">
 +
<section>
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<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>
 +
<header>
 +
<h3>Working Safely</h3>
 +
</header>
 +
<!-- TODO -->
 
 
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<p class="skill_list"><span>Skills:</span> Biology, Safety</p>
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<h4>With Parts</h4>
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</div>
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</div>
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<div style="clear:both;"></div>
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<div class="teamEntry left">
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<div class="team_image_container style5" style="background-image:url('https://static.igem.org/mediawiki/2015/6/66/Unitn_pics_team_riccardo.jpg');"></div>
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<div class="team_content">
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<p class="main_name">Riccardo Corbellari</p>
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−
<p>Hi! I’m Riccardo Corbellari, I was born in Verona and I’m 21. I’m a student of “Science in Biomolecular Sciences and Technologies” at the University of Trento. I thought I was an only child, but during this summer I found out I have 3 sisters and 3 brothers: my iGEM family! I love playing handball and watching movies. I like to fool around, for example I can’t stop teasing Alessandro, but when it’s work time no one’s more serious than me. Mainly i’m involved in pncB construction, in the pMFC realization and into designing our prototype. To sum up: how much is 15 and 18?</p>
 
 
 
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<p class="skill_list"><span>Skills:</span> Biology, Safety</p>
 
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</div>
 
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</div>
 
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<div style="clear:both;"></div>
 
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<div class="teamEntry right">
 
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<div class="team_image_container style6" style="background-image:url('');"></div>
 
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<div class="team_content">
 
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<p class="main_name">Veronica Pinamonti</p>
 
 
 
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<p>I am Veronica, I was born in Cles, a small town in a small valley near Trento. I’m attending the third year in ‘Biomolecular Sciences and Technology’ B.Sc. in Trento. I usually spend my free time with my friends at the lake. I really love science and my teammates (more or less) and this summer  I’m having  a great time in the lab with them: we laugh hard but work harder.  Mainly I’m involved in the retinal expression in E.coli. I hope you will appreciate and enjoy our project as much as I did.</p>
+
<p>All the parts we used throughout the project are NOT toxic or dangerous for humans or the environment. We neither worked with proteins toxic themselves, nor we used enzymes that synthesize toxic molecules. In addition to basic parts from the registry (i.e. RBS, promoters, β-carotene, etc), we also used two genes from the SAR86 uncultured bacteria. These genes are proteorhodopsin, which was extracted from the Registry (BBa_K773002) and blh (15,15’-β-carotene dioxygenase), which was synthesized by Genescript. There is no associated risk level with the uncultured bacteria SAR86, however we are using only DNA sequences that encodes for genes, which carry distinct functions that are no harmful to humans, or the environment. We have clarified this issue with iGEM Safety Office.</p>
 +
<p>We did NOT use any virulence factors in our organisms.</p>
 +
<p>The parts we used are to be considered safe as single parts and also as combined. Environmental dispersion of those parts would not represent a biological hazard or danger.</p>  
 
 
−
<p class="skill_list"><span>Skills:</span> Biology, Safety</p>
+
−
</div>
+
<h4>With Organisms</h4>
−
</div>
+
<p>All the bacterial strains used are from Risk Group 1. We used the following strains:</p>
−
+
<ul>
−
<div style="clear:both;"></div>
+
<li> One Shot® TOP10 Chemically Competent E. coli</li>
 +
<li> K12 - NEB 10-beta E. coli</li>
 +
<li> K12 - JM109 E. coli</li>
 +
<li> BL21 - NEB Express E. coli</li>
 +
</ul>  
 +
</section>
 +
</div>  
 
</section>
 
</section>
−
+
 +
 +
<section class="wrapper style4 container" style="margin-top:1em;">
 +
 
 +
<!-- Content -->
 +
<div class="content">
 +
<section>
 +
<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>
 +
<header>
 +
<h3>Personal Protective Equipment (PPE)</h3>
 +
</header>
 +
 +
<p>Last but non least we adopted the appropriate PPE during the lab work.  We always used lab coats, nitrile gloves and goggles.  We safely operated with biological material under the laminar flow cabinet BioAir SAFEMATE Series Class II 1.8.</p>
 +
<p>We used chemicals that are associated with hazard (ethidium bromide, azide,  organic solvents) under the chemical hood Fume Hood MOD. ASEM® 120EN New - Class 0.</p>
 +
<p>We carefully revised all the Material Safety Datasheet of each compound that was  used prior to start an experiment.</p> 
 +
</section>
 +
</div>
 +
</section>
 +
 
 +
<!-- Two -->
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 +
 
 
</article>
 
</article>
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<!-- Footer -->
 
<!-- Footer -->

Revision as of 10:15, 10 August 2015

Work hard, Work safe

?

We have reviewed our organisms and parts against the White List. All the parts and bacterial strains that we used are in the Risk Group 1

We have submitted to iGEM headquarters “About Our Lab” and “About Our Project” questionnaires by the June 26th deadline

We have submitted to iGEM headquarters the “Final Safety Form” by the August 28th deadline

About Safety

We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:

  • General considerations on risks and prevention
  • Electrical Hazard
  • Chemical Hazard
  • Biological Hazard
  • Compressed gasses and cryogenic liquids

We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.

Safe Project Design

Who will use your product? What opinions do these people have about your project?

ANSWER

Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?

ANSWER

If your product is successful, who will receive benefits and who will be harmed?

What happens when it's all used up? Will it be sterilized, discarded, or recycled?

Is it safer, cheaper, or better than other technologies that do the same thing?

Working Safely

With Parts

All the parts we used throughout the project are NOT toxic or dangerous for humans or the environment. We neither worked with proteins toxic themselves, nor we used enzymes that synthesize toxic molecules. In addition to basic parts from the registry (i.e. RBS, promoters, β-carotene, etc), we also used two genes from the SAR86 uncultured bacteria. These genes are proteorhodopsin, which was extracted from the Registry (BBa_K773002) and blh (15,15’-β-carotene dioxygenase), which was synthesized by Genescript. There is no associated risk level with the uncultured bacteria SAR86, however we are using only DNA sequences that encodes for genes, which carry distinct functions that are no harmful to humans, or the environment. We have clarified this issue with iGEM Safety Office.

We did NOT use any virulence factors in our organisms.

The parts we used are to be considered safe as single parts and also as combined. Environmental dispersion of those parts would not represent a biological hazard or danger.

With Organisms

All the bacterial strains used are from Risk Group 1. We used the following strains:

  • One Shot® TOP10 Chemically Competent E. coli
  • K12 - NEB 10-beta E. coli
  • K12 - JM109 E. coli
  • BL21 - NEB Express E. coli

Personal Protective Equipment (PPE)

Last but non least we adopted the appropriate PPE during the lab work. We always used lab coats, nitrile gloves and goggles. We safely operated with biological material under the laminar flow cabinet BioAir SAFEMATE Series Class II 1.8.

We used chemicals that are associated with hazard (ethidium bromide, azide, organic solvents) under the chemical hood Fume Hood MOD. ASEM® 120EN New - Class 0.

We carefully revised all the Material Safety Datasheet of each compound that was used prior to start an experiment.