Difference between revisions of "Team:UNITN-Trento/Test"

m
Line 76: Line 76:
 
<span>We are UNITN iGEM 2015 and we are such essence as dNTPs are made on<br/>
 
<span>We are UNITN iGEM 2015 and we are such essence as dNTPs are made on<br/>
 
 
−
<a class="btn" href="#"> To->Team </a></span></p>
+
<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Team"> Team </a></span></p>
 
</li>
 
</li>
 
<li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/0/04/Unitn_pics_slider_5.jpg'); ">
 
<li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/0/04/Unitn_pics_slider_5.jpg'); ">
Line 82: Line 82:
 
<span>Many techniques, different strains and so much fluorescence… Enjoy the Measurement Interlab Study<br/>
 
<span>Many techniques, different strains and so much fluorescence… Enjoy the Measurement Interlab Study<br/>
 
 
−
<a class="btn" href="#"> To->Interlab </a></span></p>
+
<a class="btn" href="#"> Interlab Study </a></span></p>
 
</li>
 
</li>
 
<li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/5/58/Unitn_pics_slider_6.jpg'); ">
 
<li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/5/58/Unitn_pics_slider_6.jpg'); ">
 
<p class="pintest">Synthetic Biology and mountains<br/>
 
<p class="pintest">Synthetic Biology and mountains<br/>
 
<span>Admire our lovely city, surrounded by mountains. Trento is waiting for you<br/>
 
<span>Admire our lovely city, surrounded by mountains. Trento is waiting for you<br/>
−
<a class="btn" href="#"> To->Interlab </a></span></p>  
+
<a class="btn" href="http://www.unitn.it/en" target="_blank"> University of Trento </a></span></p>  
 
</li>
 
</li>
 
</ul>
 
</ul>
Line 103: Line 103:
 
<span class="rotate-box-icon"><i class="faa flaticon-dna17"></i></span>
 
<span class="rotate-box-icon"><i class="faa flaticon-dna17"></i></span>
 
<div class="rotate-box-info">
 
<div class="rotate-box-info">
−
<h4># new BioBricks</h4>
+
<h4><span style="font-size:3em;">8</span> new BioBricks</h4>
−
<p>We built and submitted a total number of new BioBricks and characterized # of them. Check out our [Parts] page to know more!</p>
+
<p>We built and submitted a total number of new BioBricks. Check out our Parts page to know more!</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 113: Line 113:
 
<span class="rotate-box-icon"><i class="faa flaticon-tools6"></i></span>
 
<span class="rotate-box-icon"><i class="faa flaticon-tools6"></i></span>
 
<div class="rotate-box-info">
 
<div class="rotate-box-info">
−
<h4>Improved # Parts</h4>
+
<h4>Improved <span style="font-size:3em;">2</span> Parts</h4>
−
<p>Something existing, reshaped better. # now works!</p>
+
<p>Something existing, reshaped better. Now they work better!</p>
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 12:28, 10 August 2015

Electron production with E. coli and proteorhodopsin


The aim of our project is to develop a pseudoautotrophic E. coli, enabling it to live in the microbial fuel cell anaerobic condition. Our idea is to engineer E. coli with proteorhodopsin, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with blh, that encodes for the enzyme that cleaves beta-carotene and produces retinal.

To increase electron production we plan to overexpress pncB that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production. Our bacterial device will give rise to a self-sustainable system, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.

Achievements

8 new BioBricks

We built and submitted a total number of new BioBricks. Check out our Parts page to know more!

Improved 2 Parts

Something existing, reshaped better. Now they work better!

Measurements Interlab Study

We are participating also to the Measurements Interlab Study. Check out our progress in the Interlab Section!