Difference between revisions of "Team:San Andres/Parts"

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   <!--navigation menu -->
 
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   <br>
 
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    <h1>Parts&nbsp;</h1>
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      </tr>
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    <big>Throughout the project we started to learn the fundamental
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          <a href="https://2015.igem.org/Team:San_Andres" style="color: rgb(0, 0, 0);">Home </a>
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          <a href="https://2015.igem.org/Team:San_Andres/Team" style="color: rgb(0, 0, 0);"> Team</a>
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          <a href="https://2015.igem.org/Team:San_Andres/Notebook" style="color: rgb(0, 0, 0);"> Notebook</a>
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          <a href="https://2015.igem.org/Team:San_Andres/Description" style="color: rgb(0, 0, 0);"> Project </a>
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          <a href="https://2015.igem.org/Team:San_Andres/Parts" style="color: rgb(0, 0, 0);"> Parts</a>
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          <a href="https://2015.igem.org/Team:San_Andres/Modeling" style="color: rgb(0, 0, 0);">Modeling</a>
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          <a href="https://2015.igem.org/Team:San_Andres/Results" style="color: rgb(0, 0, 0);"> Results</a>
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          <a href="https://2015.igem.org/Team:San_Andres/Practices" style="color: rgb(0, 0, 0);"> Human Practices</a>
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          <a href="https://2015.igem.org/Team:San_Andres/Design" style="color: rgb(0, 0, 0);"> Future Projections</a>
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        </td>
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        <td style="border: 2px solid black;" align="center" bgcolor="#ffa600" height="30" onmouseout="this.bgColor='#FFA600'" onmouseover="this.bgColor='#ffba00'">
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          <a href="https://2015.igem.org/Team:San_Andres/Collaborations" style="color: rgb(0, 0, 0);"> Collaborations</a>
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        </td>
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      </tr>
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    </tbody>
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  </table>
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<div class="gwd-div-2ed9"></div>
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<div class="gwd-div-7xzo gwd-a-1thu">
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<h1>Parts&nbsp;</h1>
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−
<big>Throughout the project we started to learn the fundamental
+
 
principles of synthetic biology to get to work on our plasmid with
 
principles of synthetic biology to get to work on our plasmid with
 
which we want to see gluten degradation via the enzyme Kumamax. For
 
which we want to see gluten degradation via the enzyme Kumamax. For
Line 613: Line 559:
 
make our future bacteria can degrade gluten. The parts are:<br>
 
make our future bacteria can degrade gluten. The parts are:<br>
 
</big>
 
</big>
−
<ul>
+
    <ul>
−
  <li><big><span
+
      <li><big><span style="font-family: 'Arial','sans-serif';"><strong style="font-weight: bold;">Promoter
−
style="font-family: 'Arial','sans-serif';"><strong
+
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a href="http://parts.igem.org/Part:BBa_J23119"><span style="font-weight: bold;">)</span></a>:
−
style="font-weight: bold;">Promoter
+
−
     <a href="http://parts.igem.org/Part:BBa_J23119">(BBa_J23119</a></strong><a
+
−
href="http://parts.igem.org/Part:BBa_J23119"><span
+
−
style="font-weight: bold;">)</span></a>:
+
 
     </span>Constitutive
 
     </span>Constitutive
 
promoter (which works permanently) that is give in the relative
 
promoter (which works permanently) that is give in the relative
−
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li>
+
fluorescence of these plasmids in the TG1 strain grown in LB medium.</big>
−
  <li><big><strong style="font-weight: bold;">RBS</strong><strong
+
      </li>
−
style="font-weight: bold;">
+
      <li><big><strong style="font-weight: bold;">RBS</strong><strong style="font-weight: bold;">
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
     <a href="http://parts.igem.org/Part:BBa_K1084103">(BBa_K1084103)</a></strong>:
 
Synthetic RBS with uplifting sequence</big>.</li>
 
Synthetic RBS with uplifting sequence</big>.</li>
−
  <li><big><strong>Vector:<span
+
      <li><big><strong>Vector:<span style="font-weight: normal;"> <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big>
−
style="font-weight: normal;"> <a
+
      </li>
−
href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></span></strong></big></li>
+
      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Coding Region:
−
  <li><big><strong><span
+
−
style="font-family: 'Arial','sans-serif';">Coding Region:
+
 
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
 
KumaMax <a href="http://parts.igem.org/Part:BBa_K590087">(BBa_K590087)</a>:</span></strong>
 
It
 
It
 
degrades gluten,
 
degrades gluten,
 
celiac disease leading cause. Enzyme generated by rational mutation for
 
celiac disease leading cause. Enzyme generated by rational mutation for
−
the active site of it. It was created by the team IGEM <a
+
the active site of it. It was created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
−
href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
+
2011.</a></big>
−
2011.</a></big></li>
+
      </li>
−
  <li><big><strong><span
+
      <li><big><strong><span style="font-family: 'Arial','sans-serif';">Reporter: RFP
−
style="font-family: 'Arial','sans-serif';">Reporter: RFP
+
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span style="font-family: Arial,sans-serif;"> Red f</span>luorescence
−
     <a href="http://parts.igem.org/Part:BBa_J04450">(BBa_J04450)</a>:</span></strong><span
+
protein.</big>
−
style="font-family: Arial,sans-serif;"> Red f</span>luorescence
+
      </li>
−
protein.</big></li>
+
      <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
−
  <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
+
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 
     <a href="http://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a>:</strong>
 
     </span>Dual terminator consisting of
 
     </span>Dual terminator consisting of
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
the B0010 and B0012 parties. It serves to give greater efficiency in
 
transcription</big>.</li>
 
transcription</big>.</li>
−
</ul>
+
    </ul>
−
<div style="text-align: center;"><img
+
    <div style="text-align: center;">
−
alt="File:Parts.jpg"
+
      <img alt="File:Parts.jpg" src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg" height="59" width="288">
−
src="https://static.igem.org/mediawiki/2015/e/e8/Parts.jpg"
+
      <br>
−
height="59" width="288"><br>
+
      <img alt="File:Circuito.jpg" src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg" height="309" width="800">
−
<img alt="File:Circuito.jpg"
+
      <br>
−
src="https://static.igem.org/mediawiki/2015/thumb/2/2a/Circuito.jpg/800px-Circuito.jpg"
+
      <div style="text-align: left;"><big>This is a
−
height="309" width="800"><br>
+
−
<div style="text-align: left;"><big>This is a
+
 
graphic model of as it has be our plasmid where we can visualize the
 
graphic model of as it has be our plasmid where we can visualize the
 
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
 
promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
 
joined by means of prefixes and suffixes that indicate the locations of
 
joined by means of prefixes and suffixes that indicate the locations of
−
court.</big><br>
+
court.</big>
−
<br>
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        <br>
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<div style="text-align: center;"><img
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        <br>
−
alt="File:Plasmido.jpg"
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−
src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg"
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          <img alt="File:Plasmido.jpg" src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg" height="600" width="664">
−
height="600" width="664"></div>
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        </div>
−
</div>
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      </div>
−
</div>
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    </div>
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<big><br>
+
    <big><br>
 
</big>
 
</big>
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  </div>
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<br>
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  <br>
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   <a href="https://2015.igem.org/Main_Page"
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class="gwd-a-3xpx gwd-a-36ww">
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    <img style="border: 0px solid ; width: 150px; height: 150px; top: 35px; left: 989px;" alt="" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" class="gwd-img-kwqf">
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<img
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style="border: 0px solid ; width: 150px; height: 150px; top: 35px; left: 989px;"
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    <img src="https://static.igem.org/mediawiki/2015/thumb/9/94/Logo43.png/454px-Logo43.png" class="gwd-img-l2p5">
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  <br>
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class="gwd-img-l2p5">
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</a><br>
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      body {
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        font-family: Arial;
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        line-height: 3rem;
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        position: relative;
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      nav li ul {
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      nav a {
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        color: rgb(0, 0, 0);
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        display: block;
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        padding: 0px 2.5em;
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        text-decoration: none;
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        transition: 0.3s;
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        -webkit-transition: 0.3s;
 +
      }
 +
      nav a:hover {
 +
        background: rgb(255, 186, 0);
 +
      }
 +
      nav li ul {
 +
        display: none;
 +
      }
 +
      nav li:hover ul {
 +
        display: block;
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 +
      nav > ul {
 +
        display: table;
 +
        width: 100%;
 +
        background: rgb(255, 166, 0);
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      }
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      nav > ul > li {
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        float: left;
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      nav li li {
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        max-height: 0px;
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        transition: all 0.1s;
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        -webkit-transition: all 0.1s;
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        overflow: hidden;
 +
        width: 200px;
 +
        background: rgb(237, 173, 0);
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      nav li:hover > ul > li {
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        max-height: 500px;
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      nav > ul > li > ul {
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 +
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 +
    </style>
 +
    <nav class="gwd-nav-nvls">
 +
      <ul>
 +
        <li><a href="https://2015.igem.org/Team:San_Andres">Home</a>
 +
        </li>
 +
        <li><a href="https://2015.igem.org/Team:San_Andres/Team">Team</a>
 +
        </li>
 +
        <li><a href="https://2015.igem.org/Team:San_Andres/Description">Project</a>
 +
          <ul>
 +
            <li><a href="https://2015.igem.org/Team:San_Andres/Parts">Parts</a>
 +
            </li>
 +
            <li><a href="https://2015.igem.org/Team:San_Andres/Modeling">Modeling</a>
 +
            </li>
 +
            <li><a href="https://2015.igem.org/Team:San_Andres/Results">Results</a>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
        <li><a href="https://2015.igem.org/Team:San_Andres/Notebook">Notebook</a>
 +
          <ul>
 +
            <li><a href="https://2015.igem.org/Team:San_Andres/Practices">Human Practices</a>
 +
            </li>
 +
            <li><a href="https://2015.igem.org/Team:San_Andres/Design">Future Projections</a>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
        <li><a href="https://2015.igem.org/Team:San_Andres/Collaborations">Collaborations</a>
 +
        </li>
 +
      </ul>
 +
    </nav>
 +
  </div>
 
</body>
 
</body>
 +
 
</html>
 
</html>

Revision as of 00:05, 14 August 2015

wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pSB1C3
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it. It was created by the team IGEM Washington 2011.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:Parts.jpg
File:Circuito.jpg
This is a graphic model of as it has be our plasmid where we can visualize the promoter, the RBS, the enzyme KumaMax, the RFP and the terminator, joined by means of prefixes and suffixes that indicate the locations of court.

File:Plasmido.jpg