Difference between revisions of "Team:San Andres/Software"

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     <h1>Enzymes</h1>
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     <h1>Results&nbsp;</h1>
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     <big>During our investigation we sought the perfect enzyme to
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     <big>The DNA sequences of the proteins KumaMax and RFP are
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degrade the gluten, and we found:<br>
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isolated to begin building our plasmid.<br>
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<br>
 
</big>
 
</big>
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    <ul>
 
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      <li><big>Prolyl Endopeptidase: <span style="" lang="EN-US">The prolyl
 
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endopeptidase (PEP), is a class of serine-protease able to break
 
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peptide bonds
 
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following a PROLINE residue terminal carboxyl group. Were studying it
 
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its use
 
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as a therapeutic agent against celiac disease (CD), characterized by
 
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atrophy of
 
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the intestinal villi and inflammation. These reactions are attributed
 
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to
 
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peptides rich in PROLINE that are generated during the digestion of
 
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gluten of
 
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some cereals. This enzyme could be used as a nutritional supplement for
 
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individuals who suffer from celiac disease or during the processing of
 
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starches
 
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produced from cereals containing gluten. While it was a candidate for a
 
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possible treatment failed to meet expectations, because its activity is
 
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to a
 
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high pH, which is not suitable for an average digestive. Another reason
 
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was
 
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that degrades the gluten slowly, which would have resulted in a longer
 
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and
 
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inefficient treatment.</span><big>&nbsp;</big></big>
 
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      </li>
 
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      <br>
 
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    </ul>
 
 
     <div style="text-align: center;">
 
     <div style="text-align: center;">
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       <img alt="File:Prolil.jpg" src="https://static.igem.org/mediawiki/2015/c/c0/Prolil.jpg" height="376" width="565">
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       <img alt="File:Biobricks.jpg" src="https://static.igem.org/mediawiki/2015/c/c5/Biobricks.jpg" height="249" width="412">&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
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       <br>
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       <img style="width: 395px; height: 250px;" alt="File:Electroforesis.png" src="https://static.igem.org/mediawiki/2015/b/bc/Electroforesis.png">
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    </div>
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    <div style="text-align: center;">
 
       <br>
 
       <br>
 
       <div style="text-align: left;">
 
       <div style="text-align: left;">
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         <ul>
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         <br>
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          <li><big>Kumamolisin As: It is the first known example of
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         <br>
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a collagenase derived from the family of the sedolisin. This operates
+
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at high temperatures and low pH levels. Its characteristics, together
+
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with those predicted are measured by comparison between a collagenase
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and a peptidase from serine, which are related to the enzyme
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preference, to thus Digest collagen as gluten.</big>
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          </li>
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         </ul>
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         <div style="text-align: center;">
 
         <div style="text-align: center;">
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          <img alt="File:2-2-2 2.jpg" src="https://static.igem.org/mediawiki/2015/b/be/2-2-2_2.jpg" height="243" width="470">
 
 
           <br>
 
           <br>
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          <ul style="text-align: left;">
 
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            <li><big>KumaMax (G319S, D358G, D368H, N281D): It is a
 
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mutation of the Kumamolisin As, which is designed to digest way more
 
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efficient gluten, because that can work at pH levels much more lower
 
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than the original enzyme (a pH of 4.0) which is excellent for the
 
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average digestive system. <span class="hps">It was
 
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created by the team IGEM <a href="https://2011.igem.org/Team:Washington/Celiacs/Background">Washington
 
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2011</a></span>. Other advantages
 
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are:</big>
 
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            </li>
 
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          </ul>
 
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          <ol style="text-align: left;">
 
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            <li><big>It is resistant to high temperatures and acidity
 
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of the stomach.</big>
 
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            </li>
 
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            <li><big>It is heat stable, in others words, it is
 
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resistant to all changes in their physical and chemical structure.</big>
 
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            </li>
 
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            <li><big>It is easily repairable and creable.</big>
 
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            </li>
 
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          </ol>
 
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          <div style="text-align: center;">
 
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            <img alt="File:175px-Washington Bottle.jpg" src="https://static.igem.org/mediawiki/2015/d/d0/175px-Washington_Bottle.jpg" height="263" width="175">
 
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            <img alt="File:250px-Washington Kumamolisin VS SC-PEP.png" src="https://static.igem.org/mediawiki/2015/1/1b/250px-Washington_Kumamolisin_VS_SC-PEP.png" height="213" width="250">&nbsp;
 
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            <img alt="File:250px-Washington Kuma Bonded triad.png" src="https://static.igem.org/mediawiki/2015/6/62/250px-Washington_Kuma_Bonded_triad.png" height="193" width="250">
 
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            <br>
 
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            <br>
 
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            <h1 style="border-bottom: 1px solid rgb(170, 170, 170); margin: 0px 0px 0.6em; background: transparent none repeat scroll 0% 50%; -moz-background-clip: initial; -moz-background-origin: initial; -moz-background-inline-policy: initial; color: rgb(0, 0, 0); font-weight: normal; padding-top: 0.5em; padding-bottom: 0.17em; font-size: 23.876px; font-family: sans-serif; font-style: normal; font-variant: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;">Metodology&nbsp;</h1>
 
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            <div style="text-align: left;"><big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;">The
 
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methodology consists of:<br>
 
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</big>
 
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              <p class="MsoNormal"><big><span style="" lang="EN-US">- For our
 
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Plasmid construction, we decided to take the method standard of
 
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Assembly
 
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biobrick, based on grafts and vectors, front and reverse.<o:p></o:p></span></big>
 
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              </p>
 
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              <p class="MsoNormal"><big><span style="" lang="EN-US">- Then we
 
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cut the two genes and making them again an insert front, which we took
 
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to the
 
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vector of the terminator making as well as the genes a front vector.<o:p></o:p></span></big>
 
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              </p>
 
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              <p class="MsoNormal"><big><span style="" lang="EN-US">- After
 
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making two front inserts, we proceed to start with two reverse inserts
 
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for
 
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where we cut the three genes (Kumamax, RFP, terminator) and we make
 
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them a
 
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reverse insert, and we took him to the inverse vector of the RBS.<o:p></o:p></span></big>
 
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              </p>
 
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              <p class="MsoNormal"><span style="" lang="EN-US"><big>-
 
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Finally
 
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we proceed to cut the four genes (RBS, Kumamax, RFP, terminator) as
 
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insert
 
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reverse, to take them to the inverse vector of the promoter, and we
 
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finished
 
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building our final plasmid, the "Kumamax Plux".</big><o:p></o:p></span>
 
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              </p>
 
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              <p class="MsoNormal"><span style="" lang="EN-US"><o:p></o:p></span>
 
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              </p>
 
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            </div>
 
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              <img class="shrinkToFit" alt="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" src="https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg" style="border: medium none ; vertical-align: middle;" height="657" width="755">
 
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              <div style="text-align: left;"><big>At this time we
 
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are innovating ideas to add a circuit that will allow us in the future
 
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to obtain a method of detecting and quantifying the presence of gluten,
 
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which can also be checked by a commercial kit.</big>
 
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                <br>
 
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                <br>
 
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                <div style="text-align: center;">
 
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                  <img alt="File:Kit gluten.jpg" src="https://static.igem.org/mediawiki/2015/0/09/Kit_gluten.jpg" style="border: medium none ; vertical-align: middle;" height="513" width="593">
 
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            <big style="color: rgb(0, 0, 0); font-family: sans-serif; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px;"><br>
 
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</big><span style="color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 19.05px; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 1; word-spacing: 0px; display: inline ! important; float: none; background-color: rgb(255, 255, 255);"></span>
 
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Revision as of 00:26, 14 August 2015

wiki wiki exp wiki wiki wiki 2   File:Gluten-s-Job.jpeg

Results 

The DNA sequences of the proteins KumaMax and RFP are isolated to begin building our plasmid.
File:Biobricks.jpg          File:Electroforesis.png