Difference between revisions of "Team:UiOslo Norway/Experiments"
| Line 15: | Line 15: | ||
<li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/TOPO_cloning">TOPO Zero blunt cloning (Invitrogen) </a></li> | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/TOPO_cloning">TOPO Zero blunt cloning (Invitrogen) </a></li> | ||
<li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/PCR_Cleanup">Wizard® SV Gel and PCR Clean-Up System (Promega) </a></li> | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/PCR_Cleanup">Wizard® SV Gel and PCR Clean-Up System (Promega) </a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/ | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Miniprep">QIAprep Spin Miniprep Kit (Qiagen) </a></li> |
| − | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/ | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/NucleoSpin_Tissue">NucleoSpin® Tissue (Machery-Nagle) </a></li> |
| − | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/ | + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Mutagenesis">In vitro Mutagenesis </a></li> |
| + | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Agarose_Gel">Agarose Gel </a></li> | ||
</ul> | </ul> | ||
<h3> Protein expression </h3> | <h3> Protein expression </h3> | ||
Revision as of 07:02, 14 August 2015
Protocols
Molecular biology
- PCR with Phusion-HF DNA Polymerase (NEB)
- PCR with Taq DNA Polymerase (NEB)
- Restriction Digest
- Shrimp Alkaline Phosphatase Treatment (Fermentas)
- Ligation with T4 DNA Polymerase (NEB)
- TOPO Zero blunt cloning (Invitrogen)
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- QIAprep Spin Miniprep Kit (Qiagen)
- NucleoSpin® Tissue (Machery-Nagle)
- In vitro Mutagenesis
- Agarose Gel