Difference between revisions of "Team:UiOslo Norway/Experiments"
Line 23: | Line 23: | ||
<h3> Protein expression </h3> | <h3> Protein expression </h3> | ||
<h3>Handling bacteria </h3> | <h3>Handling bacteria </h3> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/E. coli strains"><i>E. coli</i> strains </a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Transformation of chemically E. coli competent cells">Transformation of chemically <i>E. coli</i> competent cells</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Concentration of Antibiotics">Concentration of antibiotics</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/LB-media">LB-media </a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/NMS-medium for growth of Methylococcus capsulatus (Bath)">NMS-medium for growth of <i>Methylococcus capsulatus</i> (Bath)</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments/Solutions for NMS medium">Solutions for NMS medium</a></li> | ||
Revision as of 09:14, 14 August 2015
Protocols
Molecular biology
- PCR with Phusion-HF DNA Polymerase (NEB)
- PCR with Taq DNA Polymerase (NEB)
- Restriction Digest
- Shrimp Alkaline Phosphatase Treatment (Fermentas)
- Ligation with T4 DNA Polymerase (NEB)
- TOPO Zero blunt cloning (Invitrogen)
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- QIAprep Spin Miniprep Kit (Qiagen)
- NucleoSpin® Tissue (Machery-Nagle)
- In vitro Mutagenesis
- Agarose Gel
- List of primers