Difference between revisions of "Team:ETH Zurich/Parts"

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<h2> Part Documentation</h2>
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<h1>Overview</h1>
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<p>For completing our project, we required of many parts. Some of these parts where used from the Registry, others were created for this project. In the following section, you can see these parts.</p>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h4>Note</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h4>Adding parts to the registry</h4>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h4>Inspiration</h4>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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</html>
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<groupparts>iGEM015 Example</groupparts>
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<h4>Registry parts </h4>
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<h2>Registry parts </h2>
  
 
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<td><b>Plasmid 3:</b></td>
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<td><b>Plasmid 3</b></td>
 
<td><b>Combination of lldR operator sites O1 and O2 and promoters of variable potency. Gene expression is blocked by LldR protein (expressed separately) and induced upon addition of L-lactate. This was tested with a fusion to GFP.</b></td>
 
<td><b>Combination of lldR operator sites O1 and O2 and promoters of variable potency. Gene expression is blocked by LldR protein (expressed separately) and induced upon addition of L-lactate. This was tested with a fusion to GFP.</b></td>
 
<td></td>
 
<td></td>

Revision as of 07:38, 26 August 2015

"What I cannot create I do not understand."
- Richard Feynmann

Overview

For completing our project, we required of many parts. Some of these parts where used from the Registry, others were created for this project. In the following section, you can see these parts.

Registry parts

Part Name

Description

Registry Number
INP-EYFP Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) BBa_K523013
cI cI repressor from E. coli phage lambda modified with an LVA tail for rapid degradation of the protein BBa_C0051
lacI + LVA lacI repressor from E. coli (+LVA) BBa_C0012
HylA HlyA-tag+Secretion system, allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli BBa_K1166002
pLuxR Promoter activated by the LuxR protein complexed with the autoinducer homoserine lactone (HSL) BBa_R0062
pLldR operon lldPRD operon promoter + RBS from E. coli. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression BBa_K822000
InterLab 1 strong promoter Anderson promoter J23101 from the Anderson collection BBa_K823005 (BBa_J23101)
InterLab 2 medium-strong promoter Anderson promoter J23106 from the Anderson collection BBa_K823008 (BBa_J23106)
terminator Transcription terminator for the E.coli RNA polymerase BBa_B0012
double terminator Double terminator consisting of BBa_B0010 and BBa_B0012 BBa_B0015
very strong promoter Strong member of the family of promoters J23100 through J23119 BBa_J23100
gfp (InterLab) intermediate in screening plasmid construction containing GFP BBa_I13504
medium promoter Medium member of the family of promoters J23100 through J23119 BBa_J23118
aiiA autoinducer inactivation enzyme aiiA (no LVA, an enzyme that inactivates the acylhomoserine lactone (AHL) quorum-sensing signal BBa_C0160
LuxI autoinducer synthetase for acylhomoserine lactone (AHL), no LVA BBa_C0161
InterLab 3 weak promoter Anderson promoter J23117 from the Anderson collection BBa_K823013 (BBa_J23117)
promoter medium-weak Medium-weak member of the family of promoters J23100 through J23119 BBa_J23114
RBS RBS.3 (medium) derivative of BBa_0030 BBa_B0032
promoter plus GFP (InterLab control) J23151 inserted in the Promoter MeasKit BBa_I20270
terminator Artificial terminator, estimated %T~>90% BBa_B1006

New constructs

Part Name

Description

Registry Number
Plasmid 3 Combination of lldR operator sites O1 and O2 and promoters of variable potency. Gene expression is blocked by LldR protein (expressed separately) and induced upon addition of L-lactate. This was tested with a fusion to GFP.
PL3a lldRO1-plldR-lldRO2
PL3b J23100-lldRO1-lldRO2
PL3c J23100-lldRO1-R2oDNA-lldRO2
PL3d J23117-lldRO1-lldRO2
PL3e J23117-lldRO1-R2oDNA-lldRO2
PL3f J23118-lldRO1-lldRO2
PL3g J23118-lldRO1-R2oDNA-lldRO2
PL3h lldRO1-J23100-lldRO2
PL3i lldRO1-J23117-lldRO2
PL3j lldRO1-J23118-lldRO2
PL3k lldRO1-R2oDNA-lldRO2