Revision as of 07:04, 26 August 2015

"What I cannot create I do not understand."
- Richard Feynmann

Parameters

Name	Value	Description	References/Estimation
\(K_{d,\text{LuxRAHL}}\)	100 nM	Dissociation constant between luxR and AHL	Weber, 2013
\(\text{LuxR}_\text{tot}\)	0.0025 μM	Total concentration of LuxR	estimated
\(a_\mathrm{LuxI}\)	1 μM.min^-1	Maximal production rate of LuxI	Basu, 2005
\(k_\mathrm{leaky}\)	0.0005 μM^-1	Leakiness of P_LuxR promoter	estimated
\(K_\mathrm{a,LuxRAHL}\)	0.01 μM	Activation coefficient of LuxRAHL	estimated
\(d_\mathrm{LuxI}\)	0.0167 min^-1	Degradation rate of LuxI	estimated
\(a_\mathrm{AHL}\)	0.04 μM.min^-1	Production rate of AHL	Weber, 2013
\(d_\mathrm{AHL}\)	0.01 min^-1	Degradation rate of AHL	Basu, 2005
\(v_\mathrm{AiiA}\)	\(k_\mathrm{cat} \cdot C_\mathrm{AiiA} \)	Maximal conversion rate of AiiA
\(k_\mathrm{cat}\)	1.63 10³min^-1	Turnover number of AiiA	Wang, 2004
\(C_\mathrm{AiiA}\)	0.05 μM	Concentration of AiiA
\(K_\mathrm{M,AiiA}\)	2.95 10³ μM	of AiiA	Wang, 2004
\(a_\mathrm{GFP}\)	2 μM.min^-1	Maximal production rate of GFP	Basu, 2005
\(d_\mathrm{GFP}\)	0.01 min^-1	Degradation rate of GFP	estimated

Name	Description	Minimum Value	Maximum Value	References/Estimation
\(\text{B}\)	\(\frac{Lac_\mathrm{ini}^2}{K_\mathrm{d,DLL}}\)	0.000001	4
\(\text{Lac}_{\text{ini}}\)	Initial concentration of lactate in the medium	0.1 μM	2 μM	Low concentration of lactate in the medium
\(K_\mathrm{d,DLL}\)	Dissociation constant between the dimer of Lldr and Lactate	10 μM²	10000 μM²
\(\alpha\)	Multiplication factor between the initial concentration of Lactate and Production of normal cells	1	150	estimated
\(F_\mathrm{C}\)	Fold change between Lactate production by cancer and normal cells	2	4	estimated
\(a_1\)	\(\frac{a_\mathrm{LacI}}{d_\mathrm{LacI}\cdot K_{RLacI}}\)	0.05	1000
\( a_\mathrm{LacI}\)	Maximal production rate of LacI	0.05 μM.min^-1	1 μM.min^-1	Basu, 2005
\( d_\mathrm{LacI}\)	Degradation rate of LacI	0.01 min^-1	0.1 min^-1	Basu, 2005
\( K_\mathrm{R,LacI}\)	Repression coefficient of LacI	0.1 μM	10 μM	Basu, 2005

@@ Line 9: / Line 9: @@
 <h3>Single cell model</h3>
 <table>
-<tr> <th>Name </th> <th>Description </th><th>Value </th><th>References/Estimation </th> </tr>
+<tr> <th>Name </th> <th>Value</th><th>Description </th><th>References/Estimation </th> </tr>
 <tr><td>\(K_{d,\text{LuxRAHL}}\)</td><td>100 nM</td><td>Dissociation constant between luxR and AHL</td><td><a href="https://2015.igem.org/Team:ETH_Zurich/References#Weber2013">Weber, 2013</a></td></tr>
 <tr><td>\(\text{LuxR}_\text{tot}\)</td><td>0.0025 &mu;M</td><td>Total concentration of LuxR </td><td>estimated</td></tr>